UBE2B (NM_003337) Human Untagged Clone

CAT#: SC127389

UBE2B (untagged)-Human ubiquitin-conjugating enzyme E2B (UBE2B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2B
• Synonyms: E2-17kDa; HHR6B; HR6B; RAD6B; UBC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127389 sequence for NM_003337 edited (data generated by NextGen Sequencing)
  ATGTCGACCCCGGCCCGGAGGAGGCTCATGCGGGATTTCAAGCGGTTACAAGAGGACCCA
  CCTGTGGGTGTCAGTGGCGCACCATCTGAAAACAACATCATGCAGTGGAATGCAGTTATA
  TTTGGACCAGAAGGGACACCTTTTGAAGATGGTACTTTTAAACTAGTAATAGAATTTTCT
  GAAGAATATCCAAATAAACCACCAACTGTTAGGTTTTTATCCAAAATGTTTCATCCAAAT
  GTGTATGCTGATGGTAGCATATGTTTAGATATCCTTCAGAATCGATGGAGTCCAACATAT
  GATGTATCTTCTATCTTAACATCAATTCAGTCTCTGCTGGATGAACCGAATCCTAACAGT
  CCAGCCAATAGCCAGGCAGCACAGCTTTATCAGGAAAACAAACGAGAATATGAGAAAAGA
  GTTTCGGCCATTGTTGAACAAAGCTGGAATGATTCATAA
  
  Clone variation with respect to NM_003337.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003337 unedited
  TCAAATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGGGGAGAGAG
  AGAACTAGTCTCGAGTTTTTTTTTTTTTTTTTTCAAACTGACCGCGGGGCAGCTGCGGAG
  CATGTCGACCCCGGCCCGGAGGAGGCTCATGCGGGATTTCAAGCGGTTACAAGAGGACCC
  ACCTGTGGGTGTCAGTGGCGCACCATCTGAAAACAACATCATGCAGTGGAATGCAGTTAT
  ATTTGGACCAGAAGGGACACCTTTTGAAGATGGTACTTTTAAACTAGTAATAGAATTTTC
  TGAAGAATATCCAAATAAACCACCAACTGTTAGGTTTTTATCCAAAATGTTTCATCCAAA
  TGTGTATGCTGATGGTAGCATATGTTTAGATATCCTTCAGAATCGATGGAGTCCAACATA
  TGATGTATCTTCTATCTTAACATCAATTCAGTCTCTGCTGGATGAACCGAATCCTAACAG
  TCCAGCCAATAGCCAGGCAGCACAGCTTTATCAGGAAAACAAACGAGAATATGAGAAAAG
  AGTTTCGGCCATTGTTGAACAAAGCTGGAATGATTCATAATAGACAACTGGTCTGTTAAT
  CTTTTTCATCATTGTTGTGTATAATTTACCTCTCATTAGAAAGGCTAACAAATTTTAAGT
  GCCACAGGTTTTAAGGATTCTGCAGAAAAAAAGAAAAAAGTCCTTCAGTTTAGAACCTAC
  AAAAGCTTGTGTATCTTGATTAATGTACTTTTTATTGCATGGTGTGAACTAAGTTATTGC
  TGCANTAAATTTAATATATCCTGTTTGTATTTTTTTCCAGTGTATATGTTTNGGTGGAGT
  TTTATGACAGATATAACATTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003337
• Insert Size: 1050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003337.2, NP_003328.1
• RefSeq Size: 2631 bp
• RefSeq ORF: 459 bp
• Locus ID: 7320
• UniProt ID: P63146
• Cytogenetics: 5q31.1
• Domains: UBCc
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008]