EGLN2 (NM_053046) Human Untagged Clone

CAT#: SC126989

EGLN2 (untagged)-Human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EGLN2
• Synonyms: EIT-6; EIT6; HIF-PH1; HIFPH1; HPH-1; HPH-3; PHD1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126989 sequence for NM_053046 edited (data generated by NextGen Sequencing)
  ATGGACAGCCCGTGCCAGCCGCAGCCCCTAAGTCAGGCTCTCCCTCAGTTACCAGGGTCT
  TCGTCAGAGCCCTTGGAGCCTGAGCCTGGCCGGGCCAGGATGGGAGTGGAGAGTTACCTG
  CCCTGTCCCCTGCTCCCCTCCTACCACTGTCCAGGAGTGCCTAGTGAGGCCTTGGCAGGG
  AGTGGGACCCCCAGAGCCACAGCCACCTCTACCACTGCCAGCCCTCTTCGGGACGGTTTT
  GGCGGGCAGGATGGTGGTGAGCTGCGGCCGCTGCAGAGTGAAGGCGCTGCAGCGCTGGTC
  ACCAAGGGGTGCCAGCGATTGGCAGCCCAGGGCGCACGGCCTGAGGCCCCCAAACGGAAA
  TGGGCCGAGGATGGTGGGGATGCCCCTTCACCCAGCAAACGGCCCTGGGCCAGGCAAGAG
  AACCAGGAGGCAGAGCGGGAGGGTGGCATGAGCTGCAGCTGCAGCAGTGGCAGTGGTGAG
  GCCAGTGCTGGGCTGATGGAGGAGGCGCTGCCCTCTGCGCCCGAGCGCCTGGCCCTGGAC
  TATATCGTGCCCTGCATGCGGTACTACGGCATCTGCGTCAAGGACAGCTTCCTGGGGGCA
  GCACTGGGCGGTCGCGTGCTGGCCGAGGTGGAGGCCCTCAAACGGGGTGGGCGCCTGCGA
  GACGGGCAGCTAGTGAGCCAGAGGGCGATCCCGCCGCGCAGCATCCGTGGGGACCAGATT
  GCCTGGGTGGAAGGCCATGAACCAGGCTGTCGAAGCATTGGTGCCCTCATGGCCCATGTG
  GACGCCGTCATCCGCCACTGCGCAGGGCGGCTGGGCAGCTATGTCATCAACGGGCGCACC
  AAGGCCATGGTGGCGTGTTACCCAGGCAACGGGCTCGGGTACGTAAGGCACGTTGACAAT
  CCCCACGGCGATGGGCGCTGCATCACCTGTATCTATTACCTGAATCAGAACTGGGACGTT
  AAGGTGCATGGCGGCCTGCTGCAGATCTTCCCTGAGGGCCGGCCCGTGGTAGCCAACATC
  GAGCCACTCTTTGACCGGTTGCTCATTTTCTGGTCTGACCGGCGGAACCCCCACGAGGTG
  AAGCCAGCCTATGCCACCAGGTACGCCATCACTGTCTGGTATTTTGATGCCAAGGAGCGG
  GCAGCAGCCAAAGACAAGTATCAGCTAGCATCAGGACAGAAAGGTGTCCAAGTACCTGTA
  TCACAGCCGCCTACGCCCACCTAG
  
  Clone variation with respect to NM_053046.3
  173 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_053046 unedited
  TAACCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCA
  GAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGA
  ATTCGGCACGAGGGCGGCTGGCACAAACGGCGGCGCCGGGGCCGGAGGAAAAAGCTCGCC
  ACCCTGAAGGGTCCCTTCCCAAGCCCTTAGGGACCGCAGAGGACTTGGGGACCAGCAAGC
  AACCCCCAGGGCACGAGAAGAGCTCTTGCTGTCTGCCCTGCCTCACCCTGCCCCACACCA
  GGCCCGGTGGCCCCCAGCTGCATCAAGTGGAGGCGGAGGAGGAGGCGGAGGAGGGTGGCA
  CCATGGGCCCGGGCGGTGCCCTCCATGCCCGGGGGATGAAGACACTGCTGCCATGGACAG
  CCCGTGCCAGCCGCAGCCCCTAAGTCAGGCTCTCCCTCAGTTACCAGGGTCTTCGTCAGA
  GCCCTTGGAGCCTGAGCCTGGCCGGGCCAGGATGGGAGTGGAGAGTTACCTGCCCTGTCC
  CCTGCTCCCCTCCTACCACTGTCCAGGAGTGCCTACTGAGGCCTTGGCAGGGAGTGGGAC
  CCCCAGAGCCACAGCCACCTCTACCACTGNCAGCCCTCTTCGGTACGGTNTTTGCGGACA
  GGATGGTGGTGAGCTGCNGTCGCTGCAAAGTGAAAGCGCTGCANNCGCTGTCACCCAAGG
  GTGCCAGCGATTGGCAGCCCAGGCGCACCGTCTGAGCCCCCAAACGGNAATGGCCCCAGA
  TGGTGGGGATGCCCTTTACCAGCAAACGGCCCTGGCCAGGCAGAGACCCAGAGGCAAACG
  GNAGGTGCATGACTGCACTGCACATGCACGGGGTAGCCATGCTGGCTATGGAGGAGGCTG
  CTTTTGCCAGACCCGGCCTGAAATATAAGGCTGCTGCGGCTAAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_053046 unedited
  CCCCCCTCCANNNAAATGGTGTGGAGTAAACTTTTTTGCTGGCTGACAGNACTCCCACCC
  ATGCTCCTCCTGGCACCTTTTACTCTGCCCAAGCAGTGGCAGCCCAGTCCAAAAGTGCCT
  GCCATGGCCAAAGGGGTTGGGGAGTCACTTCACATTCCAGGCTGGAGGAGTGAGAGGACT
  TGGGGGTACTCCTCCCACCCCATTGCCAGCAAGAGGCCATGTTTCACCTCAACCCCAAGT
  CCTGCATCCTGGTGGGAAGTGGACCATGCCTCCTTCCAGTTTTCCTTACCTAAGGGGGGA
  CATGACCAACCGGGGTAAGGAACCAGGCCCCCCAACTTCAATTTGGTAACGGGGGCCTTC
  AAGGTTGGCCCCCGTCCCGGGTAAAACCCCCCCCCCCATTAGGGGGGAAAAAAAGGGTCT
  TTTTCCCCCCTTGGGCCCCCAAAAAACAAAAAGAGCCCCCCCACCCCCGGGGGGGCCAGG
  GCCAATTTAAAACCCCCCCCCGGGGCGGGGGAAGGGGAATCCCCAAACCCCCGGGGTTCC
  TCAAAAAAATTTTAAAACTTTCTCCCCCCCCCTTTGGGGGCCGCCCCACAATTGGGGGCC
  AGGCCCCGGGGTTTTAAAAACAAATTTTTACACCCCTCTTTTCTGCGGGGGCGTTTCTAA
  ACAAACCCCCTTTTCTTTGTGGGTGGCCCCCCCCTTCTTTTCACCAAAAACAAACAACCA
  CCAAGGTGGGCCGGTTTCCCTGGGGGGCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_053046
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053046.2, NP_444274.1
• RefSeq Size: 2111 bp
• RefSeq ORF: 1224 bp
• Locus ID: 112398
• UniProt ID: Q96KS0
• Cytogenetics: 19q13.2
• Protein Families: Druggable Genome
• Protein Pathways: Pathways in cancer, Renal cell carcinoma
• Gene Summary: The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. [provided by RefSeq, Feb 2011]
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 3. Both variants 1 and 3 utilize alternative start codons that result in expression of two isoforms known as PHD1p43 and PHD1p40.