TSPAN3 (NM_005724) Human Untagged Clone

CAT#: SC126945

TSPAN3 (untagged)-Human tetraspanin 3 (TSPAN3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TSPAN3
• Synonyms: TM4-A; TM4SF8; TSPAN-3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126945 sequence for NM_005724 edited (data generated by NextGen Sequencing)
  ATGGGCCAGTGCGGCATCACCTCCTCCAAGACCGTGCTGGTCTTTCTCAACCTCATCTTC
  TGGGGGGCAGCTGGCATTTTATGCTATGTGGGAGCCTATGTCTTCATCACTTATGATGAC
  TATGACCACTTCTTTGAAGATGTGTACACGCTCATCCCTGCTGTAGTGATCATAGCTGTA
  GGAGCCCTGCTTTTCATCATTGGGCTAATTGGCTGCTGTGCCACAATCCGGGAAAGTCGC
  TGTGGACTTGCCACGTTTGTCATCATCCTGCTCTTGGTTTTTGTCACAGAAGTTGTTGTA
  GTGGTTTTGGGATATGTTTACAGAGCAAAGGTGGAAAATGAGGTTGATCGCAGCATTCAG
  AAAGTGTATAAGACCTACAATGGAACCAACCCTGATGCTGCTAGCCGGGCTATTGATTAT
  GTACAGAGACAGCTGCATTGTTGTGGAATTCACAACTACTCAGACTGGGAAAATACAGAT
  TGGTTCAAAGAAACCAAAAACCAGAGTGTCCCTCTTAGCTGCTGCAGAGAGACTGCCAGC
  AATTGTAATGGCAGCCTGGCCCACCCTTCCGACCTCTATGCTGAGGGGTGTGAGGCTCTA
  GTTGTGAAGAAGCTACAAGAAATCATGATGCATGTGATCTGGGCCGCACTGGCATTTGCA
  GCTATTCAGCTGCTGGGCATGCTGTGTGCTTGCATCGTGTTGTGCAGAAGGAGTAGAGAT
  CCTGCTTACGAGCTCCTCATCACTGGCGGAACCTATGCATAG
  
  Clone variation with respect to NM_005724.5
  603 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005724 unedited
  TGTAATACGACTCACTATAGGGCGGCCCGCGATTCGGCACGAGGGCCGCCGCCATTGCCG
  CTCTCTCGGTGAGCGCAGCCCCGCTCTCCGGGCCGGGCCTTCGCGGGCCACCGGCGCCAT
  GGGCCAGTGCGGCATCACCTCCTCCAAGACCGTGCTGGTCTTTCTCAACCTCATCTTCTG
  GGGGGCAGCTGGCATTTTATGCTATGTGGGAGCCTATGTCTTCATCACTTATGATGACTA
  TGACCACTTCTTTGAAGATGTGTACACGCTCATCCCTGCTGTAGTGATCATAGCTGTAGG
  AGCCCTGCTTTTCATCATTGGGCTAATTGGCTGCTGTGCCACAATCCGGGAAAGTCGCTG
  TGGACTTGCCACGTTTGTCATCATCCTGCTCTTGGTTTTTGTCACAGAAGTTGTTGTAGT
  GGTTTTGGGATATGTTTACAGAGCAAAGGTGGAAAATGAGGTTGATCGCAGCATTCAGAA
  AGTGTATAAGACCTACAATGGAACCAACCCTGATGCTGCTAGCCGGGCTATTGATTATGT
  ACAGAGACAGCTGCATTGTTGTGGAATTCACAACTACTCAGACTGGGAAAATACAGATTG
  GTTCAAAGAAACCAAAAACCAGAGTGTCCCTCTTAGCTGCTGCAGAGAGACTAGCCAGCA
  TTGTAATGGCAGCCTGGCCCACCCTTCCGACCTCTATGCTGANGGGTGTGAGGCTCTAGT
  TGTGAAGAAGCTACAGAAATCATGATGCATGTGATCTGGGCCGCACTGGCATTTGCAGCT
  ATTCAGCTGCTGGGCATGCTGTGTGCTTGCATCGTGTTGTGCAGAAAGAGTANAGATCCC
  TGCTACGAGCTCCTCATCACTGGCGGNAACCTATGCATAGNTGACAACTCAAGCCTGAGC
  TTTTTGGTNCTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005724 unedited
  CCCTGCTCCTCCCCTCCCCTCCNCCCNCCGCCTTCTCCCTTTTATCTCTGNNATCGCGGC
  CCGGCATTCCACGATCGAGTTTTTTTTTTTTTTTTTTGGGGGCTGCTACAGAGCTTATTG
  CAATAGATTTTTCTATTTACAACTGAGATCACATCTACATACTATTTTGCTAGTCTACAT
  GGGTCCATTATTCCCAACAAGCTTAAGACTTACCATGAATGGGCTCATTTATACAAAAAC
  ACACTCACACTAATTCTTTTAAAACAGCACTGCCTACATTATACTCCTCCTATAAAGCCA
  ACTTTGATTAAAAACCACTAGTTTCAAAGCTCAGCCTCTGATTTCGAAGATGAACCACGA
  TCTACGCCATATGATCTCACCATCTATTTTAGTCATTTCGCACAGCCGCCCATCCTATTG
  GACTACAGCAAATATTTTTTAAAAGGACCCCAATGAGGGGCACCCTCTGGCGTTAACCTT
  AACCAGAAAGCTGGCTGCCTCCTCCTTCCCTAAAAACTTTTGGCCCACCATTCTCCACTG
  TGAAGACTGCAAAGACCTGCCGAATTTGCGCGTCCCCACTGGTACCCCTTGGCCCCTACA
  CATACCCGGCCCGCGGTCCTCCTTTAACTCCACCCCCCTCCCCTGACCCCCTACCCCGGT
  TCCCACACTCCACACCCCCCCCCTTTTTTCCTCACCATTTCTTGGGCCTTGAAGAATCCC
  CTCAGACCCCGCAACACCACACCNCCCCGCTCATCGGTCAATTTTCCTTCGCCTCCCTCA
  AATACCAATTCGCGCCTTGCTCCAACACCAATTGAATTCCCTTTTCACCTAACGCACCCC
  CAGAGGGCCCAAGCTCGCCCACTGTATTTATTCCCCCCCCCCATATAAAAACGACCCATC
  CCCCTCTCGCCTAATTCGCCCCCCTATCCCAAGTTACCCCCCCCGCTTTCGCGCCCTTTT
  AAACCCCCACTCCCTTCTTCTTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005724
• Insert Size: 1730 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005724.4, NP_005715.1
• RefSeq Size: 1842 bp
• RefSeq ORF: 762 bp
• Locus ID: 10099
• UniProt ID: O60637
• Cytogenetics: 15q24.3
• Domains: transmembrane4
• Protein Families: Transmembrane
• Gene Summary: The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The use of alternate polyadenylation sites has been found for this gene. Multiple alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Dec 2009]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.