PCNA (NM_182649) Human Untagged Clone

CAT#: SC126704

PCNA (untagged)-Human proliferating cell nuclear antigen (PCNA), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCNA
• Synonyms: ATLD2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_182649, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTCGAGGCGCGCCTGGTCCAGGGCTCCATCCTCAAGAAGGTGTTGGAGGCACTCAAGGACCTCATCA
  ACGAGGCCTGCTGGGATATTAGCTCCAGCGGTGTAAACCTGCAGAGCATGGACTCGTCCCACGTCTCTTT
  GGTGCAGCTCACCCTGCGGTCTGAGGGCTTCGACACCTACCGCTGCGACCGCAACCTGGCCATGGGCGTG
  AACCTCACCAGTATGTCCAAAATACTAAAATGCGCCGGCAATGAAGATATCATTACACTAAGGGCCGAAG
  ATAACGCGGATACCTTGGCGCTAGTATTTGAAGCACCAAACCAGGAGAAAGTTTCAGACTATGAAATGAA
  GTTGATGGATTTAGATGTTGAACAACTTGGAATTCCAGAACAGGAGTACAGCTGTGTAGTAAAGATGCCT
  TCTGGTGAATTTGCACGTATATGCCGAGATCTCAGCCATATTGGAGATGCTGTTGTAATTTCCTGTGCAA
  AAGACGGAGTGAAATTTTCTGCAAGTGGAGAACTTGGAAATGGAAACATTAAATTGTCACAGACAAGTAA
  TGTCGATAAAGAGGAGGAAGCTGTTACCATAGAGATGAATGAACCAGTTCAACTAACTTTTGCACTGAGG
  TACCTGAACTTCTTTACAAAAGCCACTCCACTCTCTTCAACGGTGACACTCAGTATGTCTGCAGATGTAC
  CCCTTGTTGTAGAGTATAAAATTGCGGATATGGGACACTTAAAATACTACTTGGCTCCCAAGATCGAGGA
  TGAAGAAGGATCTTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_182649 unedited
  TCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCAGAGTGGTCGTTGTCTTTCTAGGTC
  TCAGCCGGTCGTCGCGACGTTCGCCCGCTCGCTCTGAGGCTCCTGAAGCCGAAACCAGCT
  AGACTTTCCTCCTTCCCGCCTGCCTGTAGCGGCGTTGTTGCCACTCCGCCACCATGTTCG
  AGGCGCGCCTGGTCCAGGGCTCCATCCTCAAGAAGGTGTTGGAGGCACTCAAGGACCTCA
  TCAACGAGGCCTGCTGGGATATTAGCTCCAGCGGTGTAAACCTGCAGAGCATGGACTCGT
  CCCACGTCTCTTTGGTGCAGCTCACCCTGCGGTCTGAGGGCTTCGACACCTACCGCTGCG
  ACCGCAACCTGGCCATGGGCGTGAACCTCACCAGTATGTCCAAAATACTAAAATGCGCCG
  GCAATGAAGATATCATTACACTAAGGGCCGAAGATAACGCGGATACCTTGGCGCTAGTAT
  TTGAAGCACCAAACCAGGAGAAAGTTTCAGACTATGAAATGAAGTTGATGGATTTAGATG
  TTGAACAACTTGGAATTCCAGAACAGGAGTACAGCTGTGTAGTAAAGATGCCTTCTGGTG
  AATNTGCACGTATATGCCGAGATCTCAGCCATATTGGAGATGCTNGTGTAATTTCCTGTG
  CAAAGACGGAGTGAAATTTCTGCAAGTGAGAACTTGAAATGAAAACATAANNTGTCACAG
  ACAGTATGTCGATAAGAGGAGAAAGCTGTACATAGAGATGATGAACCAGTCACTAACTTT
  GCACTGAGACCCTGACTCTTTACAAGCCACTCACTTCTCACGTGACCCTATATGCTGCGA
  AGACCCCTGTGAAAGTTAAATGCGTATGGCACTAAAACTACTGCTCCAGATCAGTAANAA
  GACTAGCATCTAATCAGAAAAACTAGCTTGAACGCTTAAAGCACATACGAACTTTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_182649 unedited
  GAACGAGGCCGCTTTTAAGTCGAGTTTTTTTTTTTTTTTTTTGAAATTCAAGTAACTTTA
  TTTAAATTCAAAAACAATTCTTAAAACTGCATTTAGAGTCAAGACCCTTTTGTATTATAA
  AAATCACAAGTATTTCTAAGAGACAAAAATACTTCTAGGTTAACTAGACCAGATCTGACT
  TTGGACTTTATTCTTTAAACAAATTGGAGAGAATAGAGAAAAAAATAGGTTATTTACAGA
  AAACAATATCTACATATGTACTTAGAGGTACAAATTTGGTGACAGAAAAGACTTCAGTAT
  ATGCTGGCATCTTAGAAGCAGTTCTCAAAGAGCTTAGTTTTATTTTCTTGAATTTTAAGA
  ATGCCTAAGATCCTTCTTCATCCTCGATCTTGGGAGCCAAGTAGTATTTTAAGTGTCCCA
  TATCCGCAATTTTATACTCTACAACAAGGGGTACATCTGCAGACATACTGAGTGTCACCG
  TTGAAGAGAGTGGAGTGGCTTTTGTAAAGAAGTTCAAGTACCTCAGTGCAAAAGTTAGTT
  GAACTGGTTCATTCATCTCTATGGTAACAGCTTCCTCCTCTTTATCGACATTACTTGTCT
  GAGACAATTTAATGTTTCCATTTCCAAGTTCTCCACTTGCAGAAAATTCACTCCGTCTTT
  TGCACAGGAAATTACAACAGCATCCTCCATATGGGCTGAGATCTCGGCATATACGTGCAA
  ATTCACCAGAAAGCATCTTTACTACACAGCTGTACTCCTGTTCTGCAATTCCAGTTGTTC
  AACTCTAAATCCTCAACCTCATTTATATCTGAAACCTCCTCCTGGTTGGGGCTCAATTCT
  ANCGCCAGGATTCGGGTTATCTCCGCCCTTATGTATGTTTCCCATTGCCGCGCATTAGTT
  TTGGGACTCCGGGAGGCCCCCCCAGGCAGTTGCGGCCAACGTAGGTTTAACCCTAACCGA
  AGGGCTGCCCAAAAACTGGACAACCTGCCTGGGTACCGGGGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_182649
• Insert Size: 1270 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182649.1, NP_872590.1
• RefSeq Size: 1319 bp
• RefSeq ORF: 786 bp
• Locus ID: 5111
• UniProt ID: P12004
• Cytogenetics: 20p12.3
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair
• Gene Summary: The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.