Endothelin B Receptor (EDNRB) (NM_000115) Human Untagged Clone

CAT#: SC126607

EDNRB (untagged)-Human endothelin receptor type B (EDNRB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Endothelin B Receptor
• Synonyms: ABCDS; ET-B; ET-BR; ETB; ETB1; ETBR; ETRB; HSCR; HSCR2; WS4A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126607 sequence for NM_000115 edited (data generated by NextGen Sequencing)
  ATGCAGCCGCCTCCAAGTCTGTGCGGACGCGCCCTGGTTGCGCTGGTTCTTGCCTGCGGC
  CTGTCGCGGATCTGGGGAGAGGAGAGAGGCTTCCCGCCTGACAGGGCCACTCCGCTTTTG
  CAAACCGCAGAGATAATGACGCCACCCACTAAGACCTTATGGCCCAAGGGTTCCAACGCC
  AGTCTGGCGCGGTCGTTGGCACCTGCGGAGGTGCCTAAAGGAGACAGGACGGCAGGATCT
  CCGCCACGCACCATCTCCCCTCCCCCGTGCCAAGGACCCATCGAGATCAAGGAGACTTTC
  AAATACATCAACACGGTTGTGTCCTGCCTTGTGTTCGTGCTGGGGATCATCGGGAACTCC
  ACACTTCTGAGAATTATCTACAAGAACAAGTGCATGCGAAACGGTCCCAATATCTTGATC
  GCCAGCTTGGCTCTGGGAGACCTGCTGCACATCGTCATTGACATCCCTATCAATGTCTAC
  AAGCTGCTGGCAGAGGACTGGCCATTTGGAGCTGAGATGTGTAAGCTGGTGCCTTTCATA
  CAGAAAGCCTCCGTGGGAATCACTGTGCTGAGTCTATGTGCTCTGAGTATTGACAGATAT
  CGAGCTGTTGCTTCTTGGAGTAGAATTAAAGGAATTGGGGTTCCAAAATGGACAGCAGTA
  GAAATTGTTTTGATTTGGGTGGTCTCTGTGGTTCTGGCTGTCCCTGAAGCCATAGGTTTT
  GATATAATTACGATGGACTACAAAGGAAGTTATCTGCGAATCTGCTTGCTTCATCCCGTT
  CAGAAGACAGCTTTCATGCAGTTTTACAAGACAGCAAAAGATTGGTGGCTGTTCAGTTTC
  TATTTCTGCTTGCCATTGGCCATCACTGCATTTTTTTATACACTAATGACCTGTGAAATG
  TTGAGAAAGAAAAGTGGCATGCAGATTGCTTTAAATGATCACCTAAAGCAGAGACGGGAA
  GTGGCCAAAACCGTCTTTTGCCTGGTCCTTGTCTTTGCCCTCTGCTGGCTTCCCCTTCAC
  CTCAGCAGGATTCTGAAGCTCACTCTTTATAATCAGAATGATCCCAATAGATGTGAACTT
  TTGAGCTTTCTGTTGGTATTGGACTATATTGGTATCAACATGGCTTCACTGAATTCCTGC
  ATTAACCCAATTGCTCTGTATTTGGTGAGCAAAAGATTCAAAAACTGCTTTAAGTCATGC
  TTATGCTGCTGGTGCCAGTCATTTGAAGAAAAACAGTCCTTGGAGGAAAAGCAGTCGTGC
  TTAAAGTTCAAAGCTAATGATCACGGATATGACAACTTCCGTTCCAGTAATAAATACAGC
  TCATCTTGA
  
  Clone variation with respect to NM_000115.3
  831 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000115 unedited
  CAGATTTTGTATACGACTCCTATAGGCGGCCGCGACATTCGCCGAGGCTACACTGTCTGG
  CATTCTCGCAGCGTTTCGTCAGAGCCGGACCCGCCTGCAGCTCAAGGGAGGCGTGCTCCT
  CTCCCAGAGCAGGCTGGAACCCAGCTGGGTTCCGCCTCCCGGGAAGGTGGTCTCCATTCG
  TCGCTCTGCATCTGGTTTGTCAGATCCGAGAGGCTCTGAAACTGCGGAGCGGCCACCGGA
  CGCCTTCTGGAGCAGGTAGCAGCATGCAGCCGCCTCCAAGTCTGTGCGGACGCGCCCTGG
  TTGCGCTGGTTCTTGCCTGCGGCCTGTCGCGGATCTGGGGAGAGGAGAGAGGCTTCCCGC
  CTGACAGGGCCACTCCGCTTTTGCAAACCGCAGAGATAATGACGCCACCCACTAAGACCT
  TATGGCCCAAGGGTTCCAACGCCAGTCTGGCGCGGTCGTTGGCACCTGCGGAGGTGCCTA
  AAGGAGACAGGACGGCAGGATCTCCGCCACGCACCATCTCCCCTCCCCCGTGCCAAGGAC
  CCATCGAGATCAAGGAGACTTTCAAATACATCAACACGNGTTGTGTCCTGCCTTGTGTTC
  GTGCTGGGGATCATCGGGAACTCCACACTTCTGAGAATTATCTACAAGAACAAGTGCATG
  CGAAACGGTCCCAATATCTTGATCGCCAGCTTGGCTCTGGGAGACCTGCTGCACATCGTC
  ATTGACATCCCTATCAATGTCTACAAGCTGCTGGCAGAGGACTGGCCATTTGGAGCTGAG
  ATGTGTAAGCTGGTGCCTTTATACAGAAGCCTNCGTGGAATCACTGTGCTGAGTCATGTG
  CTCTGAGTATGACAGAATCGAGCTGTTGCTCTTGAGAGAATAAAGGAATNGGGTNCAAAT
  NACACANTAAAAATGTTGAATGGNGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000115 unedited
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGACCAGAATGTGGCA
  TTTTAATTCAATAACTTCATACTTGCTTGATAATTGTATATTTAACATAAATAATGTCCA
  CTTGTCACATTTATATTTCTTTTAAACAATCAATTAGTATTTAATGAATTAGTGTCTGTA
  CAGTGAAAAATAAGGTAGTTGTTAAAAAAACTTAAATTTTTATTGGTTTTGCTTACATAA
  TAAAAAATCAGTAACTATAGCCACTTTAGGCAACCAAAAAATCCTCCCAGATATATAATT
  TTTTACATTGTTATATTACACTTTTATAAAGTTAGCTACAGCATACCCATGTTTTGCTAT
  AAACTGTATTCCTTTGGCCATATGTAAGCTATCAATACTAAAAGGTGTTTGAACATACAT
  CCACATTAGCAGTGGATAATAAGGGAGAAAATAGGTTAAAATCTTTTATAAATCTTTTGA
  GCTGTAACATCCATGTTAACAATAATTTTGTCTGTTGCTATATTAAAGCCCTTAATACTA
  GGAAACATGTTTTGAAGTTGAACGATAGCACCTTTTTCTATCTTGGTTTTGAGTATGTAA
  AGAAATTATAATTGTTTCTTACTTATCAGTATGTTAAAGTTTTGAATCAAACATTCTTTT
  AAAAAGCCACTGAATGCAATTNTATTATANATAATGCAAGTATGCTTTTTGTGTGTAGCT
  TCAAAAATCTGCCAGCAAATTAGAGCACATGATACTGACATGCAGAACGCAAAGGTATCA
  GGATGTTAAAATTCAGCACATGGTTATACCAATAATCTACACTTTAATACGAATTAATCT
  ATTATTAACCTGCGCAATTATATTGATCCAAATTACTCTAAATGGGAGTCTAAATGACTT
  CATGTAACCGCCTCTTGAAAGTGATTGGATGAAATAAGCACAAGTCCGGGGGATCATAAA
  AGACCATAGGGGGGGCATAATATCATGCCTTCCTTTTGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000115
• Insert Size: 4130 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000115.1, NP_000106.1
• RefSeq Size: 4286 bp
• RefSeq ORF: 1329 bp
• Locus ID: 1910
• UniProt ID: P24530
• Cytogenetics: 13q22.3
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Melanogenesis, Neuroactive ligand-receptor interaction
• Gene Summary: The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]
  Transcript Variant: This variant (1) encodes isoform 1. Both variants 1 and 3 encode protein. This variant represents the use of an upstream long terminal repeat (LTR) promoter and sequence, and results in placental-specific expression. (PMID: 11054415). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.