CHRM1 (NM_000738) Human Untagged Clone

CAT#: SC126556

CHRM1 (untagged)-Human cholinergic receptor, muscarinic 1 (CHRM1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHRM1
• Synonyms: HM1; M1; M1R
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126556 sequence for NM_000738 edited (data generated by NextGen Sequencing)
  ATGAACACTTCAGCCCCACCTGCTGTCAGCCCCAACATCACCGTCCTGGCACCAGGAAAG
  GGTCCCTGGCAAGTGGCCTTCATTGGGATCACCACGGGCCTCCTGTCGCTAGCCACAGTG
  ACAGGCAACCTGCTGGTACTCATCTCTTTCAAGGTCAACACGGAGCTCAAGACAGTCAAT
  AACTACTTCCTGCTGAGCCTGGCCTGTGCTGACCTCATCATCGGTACCTTCTCCATGAAC
  CTCTATACCACGTACCTGCTCATGGGCCACTGGGCTCTGGGCACGCTGGCTTGTGACCTC
  TGGCTGGCCCTGGACTATGTGGCCAGCAATGCCTCCGTCATGAATCTGCTGCTCATCAGC
  TTTGACCGCTACTTCTCCGTGACTCGGCCCCTGAGCTACCGTGCCAAGCGCACACCCCGC
  CGGGCAGCTCTGATGATCGGCCTGGCCTGGCTGGTTTCCTTTGTGCTCTGGGCCCCAGCC
  ATCCTCTTCTGGCAGTACCTGGTAGGGGAGCGGACAGTGCTAGCTGGGCAGTGCTACATC
  CAGTTCCTCTCCCAGCCCATCATCACCTTTGGCACAGCCATGGCTGCCTTCTACCTCCCT
  GTCACAGTCATGTGCACGCTCTACTGGCGCATCTACCGGGAGACAGAGAACCGAGCACGG
  GAGCTGGCAGCCCTTCAGGGCTCCGAGACGCCAGGCAAAGGGGGTGGCAGCAGCAGCAGC
  TCAGAGAGGTCTCAGCCAGGGGCTGAGGGCTCACCAGAGACTCCTCCAGGCCGCTGCTGT
  CGCTGCTGCCGGGCCCCCAGGCTGCTGCAGGCCTACAGCTGGAAGGAAGAAGAGGAAGAG
  GACGAAGGCTCCATGGAGTCCCTCACATCCTCAGAGGGAGAGGAGCCTGGCTCCGAAGTG
  GTGATCAAGATGCCAATGGTGGACCCCGAGGCACAGGCCCCCACCAAGCAGCCCCCACGG
  AGCTCCCCAAATACAGTCAAGAGGCCGACTAAGAAAGGGCGTGATCGAGCTGGCAAGGGC
  CAGAAGCCCCGTGGAAAGGAGCAGCTGGCCAAGCGGAAGACCTTCTCGCTGGTCAAGGAG
  AAGAAGGCGGCTCGGACCCTGAGTGCCATCCTCCTGGCCTTCATCCTCACCTGGACACCG
  TACAACATCATGGTGCTGGTGTCCACCTTCTGCAAGGACTGTGTTCCCGAGACCCTGTGG
  GAGCTGGGCTACTGGCTGTGCTACGTCAACAGCACCATCAACCCCATGTGCTACGCACTC
  TGCAACAAAGCCTTCCGGGACACCTTTCGCCTGCTGCTGCTTTGCCGCTGGGACAAGAGA
  CGCTGGCGCAAGATCCCCAAGCGCCCTGGCTCCGTGCACCGCACTCCCTCCCGCCAATGC
  TGA
  
  Clone variation with respect to NM_000738.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000738 unedited
  TTTTGAATACNACTCACTATTGGCGGCCGCGAATTCGGCACGAGGGGCAGTGGGGTTGAG
  GACCCTACAGACCCCTCTTCAGCCCCGTGGTGATGACTTTCCCCTGAGGAAGCCGGGTAG
  CGTGCCTGGAGGAAGGGGCTCTCCAACCCCAGCCCCACCTAGCCACCATGAACACTTCAG
  CCCCACCTGCTGTCAGCCCCAACATCACCGTCCTGGCACCAGGAAAGGGTCCCTGGCAAG
  TGGCCTTCATTGGGATCACCACGGGCCTCCTGTCGCTAGCCACAGTGACAGGCAACCTGC
  TGGTACTCATCTCTTTCAAGGTCAACACGGAGCTCAAGACAGTCAATAACTACTTCCTGC
  TGAGCCTGGCCTGTGCTGACCTCATCATCGGTACCTTCTCCATGAACCTCTATACCACGT
  ACCTGCTCATGGGCCACTGGGCTCTGGGCACGCTGGCTTGTGACCTCTGGCTGGCCCTGG
  ACTATGTGGCCAGCAATGCCTCCGTCATGAATCTGCTGCTCATCAGCTTTGACCGCTACT
  TCTCCGTGACTCGGCCCCTGAGCTACCGTGCCAAGCGCACACNCCGCCGGGCAGCTCTTA
  TGATCGGCCTGGCCTGGCTGGTTTCCTTTGTGCTCTGGGCCCCAGCCATCCTCTTCCTTC
  ANTAACCTTGTAGGGGAGCGGACAGTGCTAGCTTGGCAGTGCTACATCCAGTTCCTCTCC
  CAGCCCATCATCACCCTTGGCACAGCCCTGGCTGCCTTCTACTCCCTGTCACAGCATGGT
  CACGCTTTATTGGCGCATCTACCGGAGACAGAGAACCAACACCGGAGCCGGGAACCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000738 unedited
  AGNANGAGCACTGGGGNAGGGTCACAGGGATGCCACCCGGGCTCTGTTCAGGAAACAGCT
  ATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTGCCCGCTGGT
  TATGTGGCTTTATTCACAGACTCAGCACTTGCTGAGGATGAAAGGAAAGAACATTTGGGG
  CCAGGCTGGGCCTGGAGATCCGGGTGGCTGGGAGGCCATGCATGGGTTGGATTGGGGAGC
  TCACAGGAGAGAACCTGCTGGCTGGGCCAGGCCTGTGTGGGGCAGGGCCGGAGGCAGGCA
  GCATGCGGGAGGCCTGGCTAGATCTGCATGGAGCAGATCCATCCAGGTGGTGGCTGTGAG
  CCAGGAGGGAGGGACTGAGGCTTCTCTGGGGCAGGGGGTATGGATTTGCGGCTGGATAGC
  AGGCACACTTGACTCCCACTCCCAGGAAAATGATATTGTAGGACAGTCAGGACACCTGGC
  TCCTGGTCCTGTTGCTGCCTCGGGACTTGATATGTGACTAGCCAAGCCGGACCCTTCCAG
  TGTCATTGTCTTGCTGATGCTTGGACATTTGCCTTGGAGTTGACCCACCTAGGGACCCTC
  TGAGGACATTTGTGGACAATCCTGGAGAGTATGCCTAGGGCCCAGGTGGGCGGGCTCTGC
  CTATGAAGCGAGGGGATTCCCCAGCTCCCTGTCATCTGGCTTTTCTCCCAGCGGCCAGTT
  CAGTTTCTGGCTGTGCCCCCCAGGGGGCACCATCTCACACCGCAATCTGGGCCGCTGCTG
  GGCCAAGGAATACTTAATGTTAAGCCTTCTTTCTCCTGGCCCGCCCTGCTGGCTCCTGAC
  TTCCTGCCCTAAGGCAACAAGGGGTCCAAAGCCCGGATCCTG
  
• Restriction Sites: Please inquire
• ACCN: NM_000738
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000738.2, NP_000729.2
• RefSeq Size: 2863 bp
• RefSeq ORF: 1383 bp
• Locus ID: 1128
• UniProt ID: P11229
• Cytogenetics: 11q12.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton
• Gene Summary: The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13. [provided by RefSeq, Jul 2008]