DNA Primase (PRIM2) (NM_000947) Human Untagged Clone

CAT#: SC125450

PRIM2 (untagged)-Human primase, DNA, polypeptide 2 (58kDa) (PRIM2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNA Primase
• Synonyms: p58; PRIM2A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125450 sequence for NM_000947 edited (data generated by NextGen Sequencing)
  ATGGAGTTTTCTGGAAGAAAGTGGAGGAAGCTGAGGTTGGCAGGTGACCAGAGGAATGCT
  TCCTACCCTCATTGCCTTCAGTTTTACTTGCAGCCACCTTCTGAAAACATATCTTTAATA
  GAATTTGAAAACTTGGCTATTGATAGAGTTAAATTGTTAAAATCAGTTGAAAATCTTGGA
  GTGAGCTATGTGAAAGGAACTGAACAATACCAGAGTAAGTTGGAGAGTGAGCTTCGGAAG
  CTCAAGTTTTCCTACAGAGAAAACTTAGAAGATGAATATGAACCACGAAGAAGAGATCAT
  ATTTCTCATTTTATTTTGCGGCTTGCTTATTGCCAGTCTGAAGAACTTAGACGCTGGTTC
  ATTCAACAAGAAATGGATCTCCTTCGATTTAGATTTAGTATTTTACCCAAGGATAAAATT
  CAGGATTTCTTAAAGGATAGCCAATTGCAGTTTGAGGCTATAAGTGATGAAGAGAAGACT
  CTTCGAGAACAGGAGATTGTTGCCTCATCACCAAGTTTAAGTGGACTTAAGTTGGGGTTC
  GAGTCCATTTATAAGATCCCTTTTGCTGATGCTCTGGATTTGTTTCGAGGAAGGAAAGTC
  TATTTGGAAGATGGCTTTGCTTACGTACCACTTAAGGACATTGTGGCAATCATCCTGAAT
  GAATTTAGAGCCAAACTGTCCAAGGCTTTGGCATTAACAGCCAGGTCCTTGCCTGCTGTG
  CAGTCTGATGAAAGACTTCAGCCTCTGCTCAATCACCTCAGTCATTCCTACACTGGCCAA
  GATTACAGTACCCAGGGAAATGTTGGGAAGATTTCTTTAGATCAGATTGATTTGCTTTCT
  ACCAAATCCTTCCCACCTTGCATGCGTCAGTTACATAAAGCCTTGCGGGAAAATCACCAT
  CTTCGTCATGGAGGCCGAATGCAGTATGGCCTATTTCTGAAGGGCATTGGTTTAACTTTG
  GAACAGGCATTGCAGTTCTGGAAGCAAGAATTTATCAAAGGAAAGATGGATCCAGACAAG
  TTTGATAAAGGTTACTCTTACAACATCCGTCACAGCTTTGGAAAGGAAGGCAAGAGGACA
  GACTATACACCTTTCAGTTGCCTGAAGATTATTCTGTCCAATCCACCAAGCCAAGGGGAT
  TATCATGGGTGCCCATTCCGTCACAGTGATCCAGAGCTGCTGAAGCAAAAGTTGCAGTCA
  TACAAGATCTCTCCTGGAGGGATAAGCCAGATTTTGGATTTAGTAAAGGGGACACATTAC
  CAGGTAGCCTGTCAAAAATACTTTGAGATGATACACAATGTGGATGATTGTGGCTTTTCT
  TTGAATCATCCTAATCAGTTCTTTTGTGAGAGCCAACGTATTCTAAATGGTGGTAAAGAC
  ATAAAGAAGGAACCTATCCAACCAGAAACTCCTCAACCCAAACCAAGTGTCCAGAAAACC
  AAGGATGCATCATCTGCTCTGGCCTCTTTAAATTCCTCTCTGGAAATGGATATGGAAGGA
  CTAGAAGATTACTTTAGTGAAGATTCTTAG
  
  Clone variation with respect to NM_000947.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000947 unedited
  GATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAACAGTGGC
  TGCCACCGTTTGTGTTTTCCCGAGTTTGAATTCTTGCAGGTGACCAAGATGGAGTTTTCT
  GGAAGAAAGTGGAGGAAGCTGAGGTTGGCAGGTGACCAGAGGAATGCTTCCTACCCTCAT
  TGCCTTCAGTTTTACTTGCAGCCACCTTCTGAAAACATATCTTTAATAGAATTTGAAAAC
  TTGGCTATTGATAGAGTTAAATTGTTAAAATCAGTTGAAAATCTTGGAGTGAGCTATGTG
  AAAGGAACTGAACAATACCAGAGTAAGTTGGAGAGTGAGCTTCGGAAGCTCAAGTTTTCC
  TACAGAGAAAACTTAGAAGATGAATATGAACCACGAAGAAGAGATCATATTTCTCATTTT
  ATTTTGCGGCTTGCTTATTGCCAGTCTGAAGAACTTAGACGCTGGTTCATTCAACAAGAA
  ATGGATCTCCTTCGATTTAGATTTAGTATTTTACCCAAGGATAAAATTCAGGATTTCTTA
  AAGGATAGCCAATTGCAGTTTGAGGCTATAAGTGATGAAGAGAAGACTCTTCGAGAACAG
  GAGATTGTTGCCTCATCACCAAGTTTAAGTGGACTTAAGTTGGGGTTCGAGTCCATTTAT
  AAGATCCCTTTTGCTGATGCTCTGGATTTGTTTCGAGGAAGGAAAGTCTATTTGGAAGAT
  GGCTTTGCTTACGTACCACTTAAGGACATTGTGGGCATCATCCTGAATGAATNTAGAGCC
  AAACTGTCCAAGGGCTTTGCATTAACAGCCCAGTCCTTGCCTGCTGTGCAGTCTGATGAA
  AGACTCAGCCTCTGCTAATCACCCAGTCATCCTACCTGGCCCAGATACAGACCCAGGAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000947 unedited
  CGGCGATGAGCCTACCCCCCCCTCACACACCCCGNGGTGGGGGTTNTAAAACAAATGTCC
  ANAAAGATGAGTCAAATACAAAGGATAANATTAAAACAAAGTTGTTCTTTATTGTAGTTT
  CTGGGGGACTTTAACCAGTCCCCAAAGCTGTTTTTATCTCCTAAAATAGGACTTAAATGA
  CATCACTCAAGTGACAGATTTTAATAGAAACTCCTCCTCTTTCCTTCTGTCAAAGCAAAA
  TATAGTGAAAGTCTCTGTTGTTACCTCAAACCTCCTTTCCTAATAAGTGTGCATGCCTGT
  AGATGGATTGGGATGCAGTGAACAGAGAAGTTAAACAAAAAGGTTAAAAAAAAAAAAAAA
  GGCCAGGCACAGTGGCTCACAACTGTAATCCCAGCACTCTGGGACGCTGAGGTGTGAGGA
  TCGCTTGAGCCCAGGAGTCTGAGATCTGCCTGGGCAACATAGGGAGACCCCCCACCTCTA
  CAAAAAAAAATTGAAAAAAATTATCTGGATATGAGGTGCACACCTGTGCTCCTAACTACT
  TGGGAGGCTGACGCCCGAGCGATCACCCGATCTGGTAAGGTCAAGGCTGCAATCACTTGT
  CACTGTGTCACTGCCCTTCTCCTTCGCGACATTGAAACTCTTTTTAACTATTTAGGCCAA
  TTCTCCTACCCCGTACCATGTTTTTTTATGAAAAAAGCCTCCTCCACCCCCCTTTTACCC
  TCCCCACCCTTCTTCTCTTATTTTTTTTTTTTCCTCTTCCCCCACCGTACTTCTATTAGT
  TTTATCCCCCCCCTTCTCTTCTTTTCTTTCTTTAATCCCCGCCCGTGTCTCATCTCCCAT
  CTCCCTTTTTCGTCTCTTCCCCTTCCCTTTTCCACTGTCCATCAATCCCCTCCTCTTTTT
  TAAACCCCTCCTTCCTTCTACTCAACCTCTCCCCCCACCCCCACCCCTCTTCTCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000947
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000947.2, NP_000938.2
• RefSeq Size: 2353 bp
• RefSeq ORF: 1530 bp
• Locus ID: 5558
• UniProt ID: P49643
• Cytogenetics: 6p11.2
• Domains: DNA_primase_lrg
• Protein Pathways: DNA replication, Metabolic pathways, Purine metabolism, Pyrimidine metabolism
• Gene Summary: This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. Alternative splicing of this gene results in multiple transcript variants. This gene has a related pseudogene, which is also present on chromosome 6. [provided by RefSeq, Apr 2014]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a).