BID (NM_001196) Human Untagged Clone

CAT#: SC125311

BID (untagged)-Human BH3 interacting domain death agonist (BID), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BID
• Synonyms: FP497
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001196, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACTGTGAGGTCAACAACGGTTCCAGCCTCAGGGATGAGTGCATCACAAACCTACTGGTGTTTGGCT
  TCCTCCAAAGCTGTTCTGACAACAGCTTCCGCAGAGAGCTGGACGCACTGGGCCACGAGCTGCCAGTGCT
  GGCTCCCCAGTGGGAGGGCTACGATGAGCTGCAGACTGATGGCAACCGCAGCAGCCACTCCCGCTTGGGA
  AGAATAGAGGCAGATTCTGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTCGCCCAGGTCG
  GGGACAGCATGGACCGTAGCATCCCTCCGGGCCTGGTGAACGGCCTGGCCCTGCAGCTCAGGAACACCAG
  CCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGCCCTGGAGCAGCTGCTGCAGGCCTACCCTAGA
  GACATGGAGAAGGAGAAGACCATGCTGGTGCTGGCCCTGCTGCTGGCCAAGAAGGTGGCCAGTCACACGC
  CGTCCTTGCTCCGTGATGTCTTTCACACAACAGTGAATTTTATTAACCAGAACCTACGCACCTACGTGAG
  GAGCTTAGCCAGAAATGGGATGGACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001196 unedited
  AAAAAGTTCAGACTTTTAAACGACTCCTTAAGGCGGCCGCCATTCGCACGAGCGAGGGCC
  CGGCCCGCTGNAGAGCACAGTGCGGGTTCTGTCGCCACTGGGACACTGTGAACCAGAGTG
  AGTCCGAGCTGCCGCGCTGCCCATGCCATGGGACTGTGAGGTCAACAACGGTTCCAGCCT
  CAGGGGATGAGTGCATCACAAACCTACTGGTGTTTGGCTTCCTCCAAAGCTTGTTCTGAC
  AACAGCTTCCGCAGAGAGCTGGACGCACTGGGCCACGAGCTGCCAGTGCTGGCTCCCCAG
  TGGGAGGGCTACCATGAGCTGCAGACTGATGGCAACCGCAGCAGCCACTCCCGCTTGGGA
  AGAATAGAGGCAGATTCTGAAAGTCAAGAAGACATCATCCGGAATATTGCCAGGCACCTC
  GCCCAGGTCGGGGACAGCATGGACCGTAGCATCCCTCCGGGCCTGGTGAACGGGCTGGCC
  CTGCAGCTCAGGAACACCANCCGGTCGGAGGAGGACCGGAACAGGGACCTGGCCACTGGC
  CTGGAGCANCTGCTGCANGCCTACCCTANAGACATGGAGAAGGAGAAGACCATGCTGGTG
  CTGGCCCTGCTGCTGGCCAANNAAGTGGCCAGTCACACGCCGTCCTTGCTCCGTGATGTC
  TTTCACACAACAGTGAATTTTATTAACCAGAACCTACGCACCTACGTGAGGAGCTTAACC
  ANAATGGGATGGACTGAACGGACAGTTCCAAAAGTGTGACTGGCTAAAGCTCGATGTGGT
  CACAGCTGTATAGCTGCTTCCCATGTANNACGAGCCCTGGCATGTGAACAGCGTTTCCTA
  GAAAAAACAGCTGGGAGATAACTGTGACTTCTATTTTAAAGACATGGTGAAACTATNACC
  CATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001196
• Insert Size: 2290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001196.2, NP_001187.1
• RefSeq Size: 2214 bp
• RefSeq ORF: 588 bp
• Locus ID: 637
• UniProt ID: P55957
• Cytogenetics: 22q11.21
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Pathways in cancer, Viral myocarditis
• Gene Summary: This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2, and thus regulate apoptosis. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Aug 2020]
  Transcript Variant: This variant (2) has an alternate 5' exon and uses a downstream in-frame start codon, as compared to variant 1. The encoded isoform 2 thus has a shorter N-terminus, as compared to isoform 1.