Histone H2A.X (H2AFX) (NM_002105) Human Untagged Clone

CAT#: SC125294

H2AFX (untagged)-Human H2A histone family, member X (H2AFX)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Histone H2A.X
• Synonyms: H2A.X; H2A/X; H2AFX
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125294 sequence for NM_002105 edited (data generated by NextGen Sequencing)
  ATGTCGGGCCGCGGCAAGACTGGCGGCAAGGCCCGCGCCAAGGCCAAGTCGCGCTCGTCG
  CGCGCCGGCCTCCAGTTCCCAGTGGGCCGTGTACACCGGCTGCTGCGGAAGGGCCACTAC
  GCCGAGCGCGTTGGCGCCGGCGCGCCAGTGTACCTGGCGGCAGTGCTGGAGTACCTCACC
  GCTGAGATCCTGGAGCTGGCGGGCAATGCGGCCCGCGACAACAAGAAGACGCGAATCATC
  CCCCGCCACCTGCAGCTGGCCATCCGCAACGACGAGGAGCTCAACAAGCTGCTGGGCGGC
  GTGACGATCGCCCAGGGAGGCGTCCTGCCCAACATCCAGGCCGTGCTGCTGCCCAAGAAG
  ACCAGCGCCACCGTGGGGCCGAAGGCGCCCTCGGGCGGCAAGAAGGCCACCCAGGCCTCC
  CAGGAGTACTAA
  
  Clone variation with respect to NM_002105.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002105 unedited
  NCCCGTTCAGATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGTCTGTT
  CTAGTGTTTGAGCCGTCGTGCTTCACCGGTCTACCTCGCTAGCATGTCGGGCCGCGGCAA
  GACTGGCGGCAAGGCCCGCGCCAAGGCCAAGTCGCGCTCGTCGCGCGCCGGCCTCCAGTT
  CCCAGTGGGCCGTGTACACCGGCTGCTGCGGAAGGGCCACTACGCCGAGCGCGTTGGCGC
  CGGCGCGCCAGTGTACCTGGCGGCAGTGCTGGAGTACCTCACCGCTGAGATCCTGGAGCT
  GGCGGGCAATGCGGCCCGCGACAACAAGAAGACGCGAATCATCCCCCGCCACCTGCAGCT
  GGCCATCCGCAACGACGAGGAGCTCAACAAGCTGCTGGGCGGCGTGACGATCGCCCAGGG
  AGGCGTCCTGCCCAACATCCAGGCCGTGCTGCTGCCCAAGAAGACCAGCGCCACCGTGGG
  GCCGAAGGCGCCCTCGGGCGGCAAGAAGGCCACCCAGGCCTCCCAGGAGTACTAAGAGGG
  CCCGCGCCGCGGCCGGCCGCCAGGCCTCCCCATGCCACCACAAAGGCCCTTTTAAGGGCC
  ACCACCGCCCTCATGGAAAGAGCTGAGCCGCTTCAGACTGCGGGGCAAGCGGGCCCGGNN
  TTCCTTTTCCCTTCCCCTTCCCTCGCCCGNCTTCGNCGNCCGGNCTNNGAGTCCCGCCCG
  CCCCCGCTCCCGTNCCGCACCGCCTGCCGNNGTCNGNCTCGGGCCCTGCCCTGTCCGCCG
  TNCGCCTTCGNGNTAGGGTCGGGCCTTTCCGATGCNGCTTTGGCGCTCTTCGGGACCTNC
  GTGGGCGCGAAGACCACCCTGCCGGGGGGGAAGGGCCGNGGNGCCNNACCTGNCCGNCCT
  CGGNGTTCGTGACTCAACCGCCCATNCCGAGTGCTAAGGGGCTGCGGGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002105
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002105.1, NP_002096.1
• RefSeq Size: 1585 bp
• RefSeq ORF: 432 bp
• Locus ID: 3014
• UniProt ID: P16104
• Cytogenetics: 11q23.3
• Domains: H2A, histone
• Protein Families: Druggable Genome
• Protein Pathways: Systemic lupus erythematosus
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]