NRG1 (NM_013956) Human Untagged Clone

CAT#: SC124810

NRG1 (untagged)-Human neuregulin 1 (NRG1), transcript variant HRG-beta1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NRG1
• Synonyms: ARIA; GGF; GGF2; HGL; HRG; HRG1; HRGA; MST131; MSTP131; NDF; NRG1-IT2; SMDF
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124810 sequence for NM_013956 edited (data generated by NextGen Sequencing)
  ATGTCCGAGCGCAAAGAAGGCAGAGGCAAAGGGAAGGGCAAGAAGAAGGAGCGAGGCTCC
  GGCAAGAAGCCGGAGTCCGCGGCGGGCAGCCAGAGCCCAGCCTTGCCTCCCCAATTGAAA
  GAGATGAAAAGCCAGGAATCGGCTGCAGGTTCCAAACTAGTCCTTCGGTGTGAAACCAGT
  TCTGAATACTCCTCTCTCAGATTCAAGTGGTTCAAGAATGGGAATGAATTGAATCGAAAA
  AACAAACCACAAAATATCAAGATACAAAAAAAGCCAGGGAAGTCAGAACTTCGCATTAAC
  AAAGCATCACTGGCTGATTCTGGAGAGTATATGTGCAAAGTGATCAGCAAATTAGGAAAT
  GACAGTGCCTCTGCCAATATCACCATCGTGGAATCAAACGAGATCATCACTGGTATGCCA
  GCCTCAACTGAAGGAGCATATGTGTCTTCAGAGTCTCCCATTAGAATATCAGTATCCACA
  GAAGGAGCAAATACTTCTTCATCTACATCTACATCCACCACTGGGACAAGCCATCTTGTA
  AAATGTGCGGAGAAGGAGAAAACTTTCTGTGTGAATGGAGGGGAGTGCTTCATGGTGAAA
  GACCTTTCAAACCCCTCGAGATACTTGTGCAAGTGCCCAAATGAGTTTACTGGTGATCGC
  TGCCAAAACTACGTAATGGCCAGCTTCTACAAGCATCTTGGGATTGAATTTATGGAGGCG
  GAGGAGCTGTACCAGAAGAGAGTGCTGACCATAACCGGCATCTGCATCGCCCTCCTTGTG
  GTCGGCATCATGTGTGTGGTGGCCTACTGCAAAACCAAGAAACAGCGGAAAAAGCTGCAT
  GACCGTCTTCGGCAGAGCCTTCGGTCTGAACGAAACAATATGATGAACATTGCCAATGGG
  CCTCACCATCCTAACCCACCCCCCGAGAATGTCCAGCTGGTGAATCAATACGTATCTAAA
  AACGTCATCTCCAGTGAGCATATTGTTGAGAGAGAAGCAGAGACATCCTTTTCCACCAGT
  CACTATACTTCCACAGCCCATCACTCCACTACTGTCACCCAGACTCCTAGCCACAGCTGG
  AGCAACGGACACACTGAAAGCATCCTTTCCGAAAGCCACTCTGTAATCGTGATGTCATCC
  GTAGAAAACAGTAGGCACAGCAGCCCAACTGGGGGCCCAAGAGGACGTCTTAATGGCACA
  GGAGGCCCTCGTGAATGTAACAGCTTCCTCAGGCATGCCAGAGAAACCCCTGATTCCTAC
  CGAGACTCTCCTCATAGTGAAAGGTATGTGTCAGCCATGACCACCCCGGCTCGTATGTCA
  CCTGTAGATTTCCACACGCCAAGCTCCCCCAAATCGCCCCCTTCGGAAATGTCTCCACCC
  GTGTCCAGCATGACGGTGTCCATGCCTTCCATGGCGGTCAGCCCCTTCATGGAAGAAGAG
  AGACCTCTACTTCTCGTGACACCACCAAGGCTGCGGGAGAAGAAGTTTGACCATCACCCT
  CAGCAGTTCAGCTCCTTCCACCACAACCCCGCGCATGACAGTAACAGCCTCCCTGCTAGC
  CCCTTGAGGATAGTGGAGGATGAGGAGTATGAAACGACCCAAGAGTACGAGCCAGCCCAA
  GAGCCTGTTAAGAAACTCGCCAATAGCCGGCGGGCCAAAAGAACCAAGCCCAATGGCCAC
  ATTGCTAACAGATTGGAAGTGGACAGCAACACAAGCTCCCAGAGCAGTAACTCAGAGAGT
  GAAACAGAAGATGAAAGAGTAGGTGAAGATACGCCTTTCCTGGGCATACAGAACCCCCTG
  GCAGCCAGTCTTGAGGCAACACCTGCCTTCCGCCTGGCTGACAGCAGGACTAACCCAGCA
  GTCCGCTTCTCGACACAGGAAGAAATCCAGGCCAGGCTGTCTAGTGTAATTGCTAACCAA
  GACCCTATTGCTGTATAA
  
  Clone variation with respect to NM_013956.3
  113 g=>a;1862 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013956 unedited
  ATACGACTCCTATAGGGCGGCCGCGAATTCGCCGAGGGGCTCGCGCGGAGGCCAGGAGCT
  GAGCGGCGGCGGCTGCCGGACGATGGGAGCGTGAGCAGGACGGTGATAACCTCTCCCCGA
  TCGGGTTGCGAGGGCGCCGGGCAGAGGCCAGGACGCGAGCCGCCAGCGGCGGGACCCATC
  GACGACTTCCCGGGGCGACAGGAGCAGCCCCGAGAGCCAGGGCGAGCGCCCGTTCCAGGT
  GGCCGGACCGCCCGCCGCGTCCGCGCCGCGCTCCCTGCAGGCAACGGGAGACGCCCCCGC
  GCAGCGCGAGCGCCTCAGCGCGGCCGCTCGCTCTCCCCATCGAGGGACAAACTTTTCCCA
  AACCCGATCCGAGCCCTTGGACCAAACTCGCCTGCGCCGAGAGCCGTCCGCGTAGAGCGC
  TCCGTCTCCGGCGAGATGTCCGAGCGCAAAGAAGGCAGAGGCAAAGGGAAGGGCAAGAAG
  AAGGAGCGAGGCTCCGGCAAGAAGCCGGAGTCCGCGGCGGGCAGCCAGAGCCCAGCCTTG
  CCTCCCCAATTGAAAGAGATGAAAAGCCAGGAATCGGCTGCAGGTTCCAAACTAGTCCTT
  CGGTGTGAAACCAGTTCTGAATACTCCTCTCTCAGATTCAAGTGGTTCAAGAATGGGAAT
  GAATTGAATCGAANAAACAAACCACAAAATATCAAGATACAAAAAAAGCCAGNGGAAGTC
  AGAACTTCGCATTAACAAAGCATCACTGGCTGATTCTGGAGAGTATATGTGCANAGTGAT
  CAGCAATTAGGAAATGACAGTGCCTCTGCCATATCACCATCGTGGATCAAACGAGATCTC
  ACTGGTATGCCAGNCTCACTGAAGAGCCTAATGTGCT
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_013956 unedited
  NTTCTCCGCGAAAAAATCAGGCACANCAGCCCACTGGGGGCCCAAAAGGACGTCTTAATG
  GCACAGGAGGCCCTNCGTGATGTAACAGCTTCCTCAGGCATGCCAGAGAAACCCCTGATT
  CCTACCGAGACTCTCCTCATAGTGAAAGGTATGTGTCAGCCATGACCACCCCGGCTCGTA
  TGTCACCTGTAGATTTCCACACGCCAAGCTCCCCCAAATCGCCCCCTTCGGAAATGTCTC
  CACCCGTGTCCAGCATGACGGTGTCCATGCCTTCCATGGCGGTCAGCCCCTTCATGGAAG
  AAGAGAGACCTCTACTTCTCGTGACACCACCAAGGCTGCGGGAGAAGAAGTTTGACCATC
  ACCCTCAGCAGTTCAGCTCCTTCCACCACAACCCCGCGCATGACAGTAACAGCCTCCCTG
  CTAGCCCCTTGAGGATAGTGGAGGATGAGGAGTATGAAACGACCCAAGAGTACGAGCCAG
  CCCAAGAGCCTGTTAAGAAACTCGCCAATAGCCGGCGGGCCAAAAGACCAAGCCCAATGG
  CCACATTGCTAACAGATTGGAAGTGGACAGCAACACAAGCTCCCAGAGCAGTAACTCAGA
  GAGTGAAACAGAAGATGAAAGAGTAGGTGAAGATACGCCTTTCCTGGGCATACAGAACCC
  CCTGGCAGCCAGTCTTGAGGCAACACCTGCCTTCCGCCTGGCTGACAGCAGGACTAACCC
  CGCAGTTCCGCTTCTCGACACAGGAAAAAATCCAGGCCACAGCTGGCTAGTGGTAATTGG
  TAACCCAAAAACCCTTTTGCTGGTTTAAAACCTAAATAAACCCCTTGGATTTCCCTGGGA
  AAACTTTATTTTTATTTTAAAAAGGTTTTTCCCCTCTAAAATAAAAACAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013956
• Insert Size: 4100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013956.2, NP_039250.2
• RefSeq Size: 2583 bp
• RefSeq ORF: 1938 bp
• Locus ID: 3084
• UniProt ID: Q02297
• Cytogenetics: 8p12
• Protein Families: Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
• Protein Pathways: ErbB signaling pathway
• Gene Summary: The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. An extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are expressed in a tissue-specific manner and differ significantly in their structure, and are classified as types I, II, III, IV, V and VI. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). [provided by RefSeq, Apr 2016]
  Transcript Variant: This variant (HRG-beta1), which uses the type I promoter, encodes isoform HRG-beta1 (also known as NDF-beta1a or HRG-beta1a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.