HLTF (NM_003071) Human Untagged Clone

CAT#: SC124775

HLTF (untagged)-Human helicase-like transcription factor (HLTF), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HLTF
• Synonyms: HIP116; HIP116A; HLTF1; RNF80; SMARCA3; SNF2L3; ZBU1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003071, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCTGGATGTTCAAGAGGGATCCAGTTTGGAAGTACTTGCAGACTGTCCAGTATGGAGTTCATGGAA
  ATTTTCCACGCCTCTCATATCCAACTTTCTTTCCACGTTTTGAATTCCAAGATGTTATCCCTCCAGATGA
  CTTTCTAACTAGTGATGAAGAAGTAGATTCCGTTTTATTTGGAAGTTTGAGAGGTCATGTGGTTGGACTA
  CGCTATTACACGGGAGTAGTTAATAATAATGAAATGGTTGCATTACAACGAGATCCTAATAACCCTTATG
  ATAAGAATGCAATTAAAGTAAACAATGTGAATGGAAATCAAGTTGGCCATTTAAAGAAAGAGCTTGCAGG
  TGCTTTGGCCTATATCATGGACAACAAATTGGCACAAATTGAAGGGGTAGTTCCTTTTGGTGCAAACAAT
  GCTTTTACCATGCCTCTGCATATGACTTTTTGGGGAAAAGAAGAAAATAGAAAAGCGGTTTCAGATCAGT
  TGAAGAAACATGGATTTAAATTGGGTCCTGCACCAAAAACTTTAGGATTCAATTTGGAAAGTGGTTGGGG
  CTCTGGAAGAGCTGGACCAAGCTATAGTATGCCAGTGCATGCTGCAGTACAGATGACAACTGAACAGCTT
  AAAACAGAATTTGACAAATTGTTTGAAGATTTAAAAGAAGATGATAAAACCCATGAAATGGAACCAGCTG
  AGGCTATTGAAACACCACTGCTTCCACATCAAAAACAAGCTCTAGCTTGGATGGTGTCACGGGAAAATAG
  CAAAGAACTTCCACCATTCTGGGAACAGCGAAATGACTTATACTATAACACAATAACAAATTTTTCTGAG
  AAGGACCGACCAGAAAATGTCCATGGAGGAATTTTAGCTGATGATATGGGTTTGGGTAAAACTCTTACGG
  CCATTGCAGTAATCCTTACCAACTTCCATGATGGCAGACCTCTTCCTATTGAAAGAGTTAAAAAGAATCT
  ACTGAAGAAGGAATATAATGTTAACGATGACTCTATGAAACTTGGAGGAAACAATACCAGTGAAAAGGCA
  GATGGACTAAGCAAAGACGCATCTAGATGTAGTGAACAACCCAGTATTTCAGATATCAAGGAGAAGAGTA
  AGTTTCGCATGTCAGAATTGTCTAGCTCCCGCCCCAAAAGAAGAAAAACTGCTGTCCAGTACATAGAAAG
  CAGTGATTCAGAGGAAATTGAAACAAGTGAATTGCCGCAGAAAATGAAAGGCAAACTGAAAAATGTACAG
  TCTGAAACTAAAGGCAGGGCGAAAGCAGGATCTTCTAAGGTTATAGAAGATGTGGCATTTGCATGTGCAT
  TAACTTCATCTGTTCCTACAACAAAAAAGAAAATGTTGAAAAAGGGAGCTTGTGCAGTGGAGGGGTCAAA
  GAAAACTGATGTTGAGGAGAGACCAAGAACAACACTGATCATCTGTCCGCTTTCTGTGTTAAGCAACTGG
  ATTGACCAGTTTGGACAACATATAAAATCAGATGTACACTTGAATTTTTATGTTTATTATGGTCCTGATC
  GTATTAGAGAACCGGCCTTACTTTCAAAACAGGATATTGTTTTGACTACGTATAATATTTTAACTCATGA
  CTATGGAACTAAAGGAGATAGTCCATTACATAGCATAAGGTGGCTAAGAGTGATCCTGGATGAAGGACAT
  GCCATACGAAATCCAAATGCTCAGCAGACAAAAGCTGTACTTGACTTAGAATCAGAAAGAAGATGGGTTT
  TGACAGGTACTCCAATCCAGAATTCTTTAAAGGACTTGTGGTCTCTTCTTTCCTTTTTAAAACTTAAACC
  ATTTATTGATAGAGAATGGTGGCATAGAACAATACAGCGTCCTGTCACAATGGGAGATGAAGGAGGACTT
  AGGCGTTTACAGTCCCTAATTAAAAATATTACACTTAGAAGAACAAAGACAAGCAAAATTAAAGGAAAAC
  CTGTTTTGGAGTTACCAGAACGTAAAGTATTTATTCAGCACATTACACTTTCAGATGAAGAGAGAAAGAT
  TTATCAGTCTGTGAAAAATGAAGGCAGAGCCACTATTGGAAGGTATTTTAATGAAGGGACTGTCCTGGCA
  CATTATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAATTTGTTGCCATACTTACCTTCTTACAA
  ATGCAGTGTCTTCCAATGGCCCCTCAGGAAATGATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAA
  GATGAAGTTAATTCTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGATTCTTTAACAGTTCCT
  GTGATAACACATTGTGCACATGTATTTTGTAAACCCTGTATTTGCCAAGTCATTCAGAATGAGCAGCCAC
  ATGCTAAATGCCCTTTATGCAGAAATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGAAGAATT
  AGCACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCAGTTCAAAGATTAATGCGCTAATGCAC
  GCATTGACTGACTTAAGAAAGAAGAATCCCAACATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCC
  TGTCTTTAATAGAAATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGGATGGTTCCATGGCCCA
  AAAGAAAAGAGTTGAATCAATTCAGTGTTTTCAAAACACTGAAGCAGGATCTCCAACTATAATGCTTCTG
  TCCTTAAAAGCAGGTGGAGTTGGTTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATCCAGCCT
  GGAATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGACTTGGTCAGAAGCAAGAAGTTATCAT
  CACAAAATTCATTGTAAAGGACTCTGTTGAAGAAAATATGCTGAAAATACAAAACAAAAAGAGAGAACTT
  GCAGCAGGAGCCTTTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCCAAAATTAATGAAATCA
  GAACATTAATTGACTTATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003071 unedited
  TCAACATATTTGTATCACGACTCACTATAGGCGGCCCGCGAATTCGCACGAGGCGGCGAC
  TTACCTTTCAGTCGTGCGCTCCTGATCCGGCGCTCGGAATTTGTCCCCGGCTTCAGGGCT
  GCGGGGCCTGGAAGGAGGCGTATCGAGGCGGCTCGAAAACGATCCAGGGGAGCCGAGGCG
  CTCCTCTTGTCATCCCACTCANCGCCATGTCCTGTATGTTCAAGAGGGATCCAGTTTGGA
  AGTACTTGCAGACTGTCCAGTATGGAGTTCATGGAAATTTTCCACGCCTCTCATATCCAA
  CTTTCTTTCCACGTTTTGAATTCCAAGATGTTATCCCTCCAGATGACTTTCTAACTAGTG
  ATGAAGAAGTAGATTCCGTTTTATTTGGAAGTTTGAGAGGTCATGTGGTTGGACTACGCT
  ATTACACGGGAGTAGTTAATAATAATGAAATGGTTGCATTACAACGAGATCCTAATAACC
  CTTATGATAAGAATGCAATTAAAGTAAACAATGTGAATGGAAATCAAGTTGGCCATTTAA
  AGAAAGAGCTTGCAGGTGCTTTGGCCTATATCATGGACAACAAATTGGCACAAATTGAAG
  GGGTAGTTCCTTTTGGTGCANACAATGCTTTTACCATGCCTCTGCATATGACCTTTTGGG
  GAAAAGAAGTAAATAGAAAAGCGGTCTCAGATCAGCTGAAGAAACATGGATTTAATTGGG
  TCCTGCACCAAAACTTTAGATTCCATTTGCAAAGGGGTTGGGCTCTTGAAAAACTGAACC
  AACTATAGTATGCCAGGCATGCTGCATTACACATGACAACTGAACCAGTTAATACAGAAT
  TTGCACAATTTGTTTGAAATTTTAAAGTAGCAGTTTAAACCTCAGNAAATGGACCAGCTG
  AGGCTATTTAAAACCCCTCGTTTCACATTCATAACAACCCCTTACTTGGTTGCGGTCACC
  GAAAATACCAAAACTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003071 unedited
  GAAATACTATGTACGCGGCCGCTTTTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTAAC
  ATTTGAAATTTTTATTGATTTTTAAATTTAAAATCCAGTTTTAACCACAAAATTGTTTGA
  ATCACAAGTGGTAATACAATGTCTTCAATATTTTTCTAAAGTTATTTTTCTATATAATAA
  TAAGACAACAGCATAGCATATAGGAAGTTTTCATTCCAGTGGCTTTTTTATATATTTATC
  CTTCTTAGGAAGGACAAATTAAATTTTTTAAATTAAACTTTTAAAATATAACAACATCTA
  ACAGAACTGTACAAAACAAAGAGACATTTTTTAAACAACTTGCCAAACTTACTTATGAGT
  GTGTTTTAAAAACAACTTTGTAAATGTCTGGGCAAAGAAGCAAGCTGTCCTCCCTTTACC
  TTCATAGTGAGTTTGTAAGGCTTTGTCTTTGTAAGCAGAAAGAGTAGACTGTGTTGTTTT
  TTGCCAAAAACTGTTTATACTTAATCTCACTGAAGTATTGCTATATGGAGAACCCATACT
  CTGATCAACTTGATTTTTTGTGTGTAATGCTTGATCTACCAGGTAACTTCCCAACTGCTC
  CTAATGCTAGCGGGCTAATCCCACATTATTATTCCACTATCATCCCTGCAGAAAGGTCTT
  GGTTTTGATGAAAATCAGCCCTTTCCTTACCTGCTACTGGCTCAAAAAGGGACCAGGAAG
  AATCTAGCTGGCTAATTCACTGTTCCCTTTGAGCAAGAAACGGCACAGGGAGAAAAAGAC
  TTATCTGGTGAGAGATTTGGCATATACCTTCCATGTGTGCCCTATAACACAACATGTCTC
  CGATCTCATCTTTCTATCCAATGACTTNCCACACTCCTAAATCTCAGGATTCTTAAATCT
  GGTCATC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003071
• Insert Size: 5500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003071.2, NP_003062.2
• RefSeq Size: 5351 bp
• RefSeq ORF: 3030 bp
• Locus ID: 6596
• UniProt ID: Q14527
• Cytogenetics: 3q24
• Domains: SNF2_N, DEAD, helicase_C, RING
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1, 2 and 3 encode the same isoform (1).