MASP2 (NM_006610) Human Untagged Clone

CAT#: SC124235

MASP2 (untagged)-Human mannan-binding lectin serine peptidase 2 (MASP2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MASP2
• Synonyms: MAP-2; MAP19; MASP-2; MASP1P1; sMAP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006610, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGGCTGCTGACCCTCCTGGGCCTTCTGTGTGGCTCGGTGGCCACCCCCTTGGGCCCGAAGTGGCCTG
  AACCTGTGTTCGGGCGCCTGGCATCCCCCGGCTTTCCAGGGGAGTATGCCAATGACCAGGAGCGGCGCTG
  GACCCTGACTGCACCCCCCGGCTACCGCCTGCGCCTCTACTTCACCCACTTCGACCTGGAGCTCTCCCAC
  CTCTGCGAGTACGACTTCGTCAAGCTGAGCTCGGGGGCCAAGGTGCTGGCCACGCTGTGCGGGCAGGAGA
  GCACAGACACGGAGCGGGCCCCTGGCAAGGACACTTTCTACTCGCTGGGCTCCAGCCTGGACATTACCTT
  CCGCTCCGACTACTCCAACGAGAAGCCGTTCACGGGGTTCGAGGCCTTCTATGCAGCCGAGGACATTGAC
  GAGTGCCAGGTGGCCCCGGGAGAGGCGCCCACCTGCGACCACCACTGCCACAACCACCTGGGCGGTTTCT
  ACTGCTCCTGCCGCGCAGGCTACGTCCTGCACCGTAACAAGCGCACCTGCTCAGCCCTGTGCTCCGGCCA
  GGTCTTCACCCAGAGGTCTGGGGAGCTCAGCAGCCCTGAATACCCACGGCCGTATCCCAAACTCTCCAGT
  TGCACTTACAGCATCAGCCTGGAGGAGGGGTTCAGTGTCATTCTGGACTTTGTGGAGTCCTTCGATGTGG
  AGACACACCCTGAAACCCTGTGTCCCTACGACTTTCTCAAGATTCAAACAGACAGAGAAGAACATGGCCC
  ATTCTGTGGGAAGACATTGCCCCACAGGATTGAAACAAAAAGCAACACGGTGACCATCACCTTTGTCACA
  GATGAATCAGGAGACCACACAGGCTGGAAGATCCACTACACGAGCACAGCGCAGCCTTGCCCTTATCCGA
  TGGCGCCACCTAATGGCCACGTTTCACCTGTGCAAGCCAAATACATCCTGAAAGACAGCTTCTCCATCTT
  TTGCGAGACTGGCTATGAGCTTCTGCAAGGTCACTTGCCCCTGAAATCCTTTACTGCAGTTTGTCAGAAA
  GATGGATCTTGGGACCGGCCAATGCCCGCGTGCAGCATTGTTGACTGTGGCCCTCCTGATGATCTACCCA
  GTGGCCGAGTGGAGTACATCACAGGTCCTGGAGTGACCACCTACAAAGCTGTGATTCAGTACAGCTGTGA
  AGAGACCTTCTACACAATGAAAGTGAATGATGGTAAATATGTGTGTGAGGCTGATGGATTCTGGACGAGC
  TCCAAAGGAGAAAAATCACTCCCAGTCTGTGAGCCTGTTTGTGGACTATCAGCCCGCACAACAGGAGGGC
  GTATATATGGAGGGCAAAAGGCAAAACCTGGTGATTTTCCTTGGCAAGTCCTGATATTAGGTGGAACCAC
  AGCAGCAGGTGCACTTTTATATGACAACTGGGTCCTAACAGCTGCTCATGCCGTCTATGAGCAAAAACAT
  GATGCATCCGCCCTGGACATTCGAATGGGCACCCTGAAAAGACTATCACCTCATTATACACAAGCCTGGT
  CTGAAGCTGTTTTTATACATGAAGGTTATACTCATGATGCTGGCTTTGACAATGACATAGCACTGATTAA
  ATTGAATAACAAAGTTGTAATCAATAGCAACATCACGCCTATTTGTCTGCCAAGAAAAGAAGCTGAATCC
  TTTATGAGGACAGATGACATTGGAACTGCATCTGGATGGGGATTAACCCAAAGGGGTTTTCTTGCTAGAA
  ATCTAATGTATGTCGACATACCGATTGTTGACCATCAAAAATGTACTGCTGCATATGAAAAGCCACCCTA
  TCCAAGGGGAAGTGTAACTGCTAACATGCTTTGTGCTGGCTTAGAAAGTGGGGGCAAGGACAGCTGCAGA
  GGTGACAGCGGAGGGGCACTGGTGTTTCTAGATAGTGAAACAGAGAGGTGGTTTGTGGGAGGAATAGTGT
  CCTGGGGTTCCATGAATTGTGGGGAAGCAGGTCAGTATGGAGTCTACACAAAAGTTATTAACTATATTCC
  CTGGATCGAGAACATAATTAGTGATTTTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006610 unedited
  GGGGGNNGGGGGGCCNNNNNNNNNNNNNNNGCTTGTTCACATTTGTATACGACTCACTAT
  AGGCGGCCGCGAAATCGGCACCAGGCCAGGCCAGCTGGACGGGCACACCATGAGGCTGCT
  GACCCTCCTGGGCCTTCTGTGTGGCTCGGTGGCCACCCCCTTGGGCCCGAAGTGGCCTGA
  ACCTGTGTTCGGGCGCCTGGCATCCCCCGGCTTTCCAGGGGAGTATGCCAATGACCAGGA
  GCGGCGCTGGACCCTGACTGCACCCCCCGGCTACCGCCTGCGCCTCTACTTCACCCACTT
  CGACCTGGAGCTCTCCCACCTCTGCGAGTACGACTTCGTCAAGCTGAGCTCGGGGGCCAA
  GGTGCTGGCCACGCTGTGCGGGCAGGAGAGCACAGACACGGAGCGGGCCCCTGGCAAGGA
  CACTTTCTACTCGCTGGGCTCCAGCCTGGACATTACCTTCCGCTCCGGCTACTCCAACGA
  GAAGCCGTTCACGGGGTTCGAGGCCTTCTATGCAGCCGAGGACATTGACGAGTGCCAGGT
  GGCCCCGGGAGAGGCGCCCACCTGCGACCACCACTGCCACAACCACCTGNGCGGTTTCTA
  CTGCTCCTGCCGCGCAGGCTACGTCCTGCACCGTAACAAGCGCACCTGCTCAGCCCTGTG
  CTCCGGCCAGGTCTTCACCCAGAGGTCTGGGGAGCTCAGCAGCCCTGAATACCCACGGCC
  GTATCCCAAACTCTNCAGTTGCACTTACAGCATCAGCCTGGAGGAAGGGTTCAGTGTCAT
  TCTGGACTTTGTGGATCCTTCGATGTGGAGACACACCCTGAAACCCTGTGTCCCTACGAC
  TTNTCTCAGATCAAACAGACAGAGAAGACATGCCCCATTCTGNGGGAAGACATTGCCCCA
  CG
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_006610 unedited
  ANCATGGNACAGGNNCAGTTTACAGNAAATGCCAACAGCCAGTATGAAAAGAGACTGGCT
  TTTTAAGGTAATGAAATGTAATTTGAGCAGTGACATTACACTGGGTGGGCAAAGGTGACT
  GTCACTCTCGTGGTTTATGTCCCCTTGAGTCAATGGGTAAGGCTGGAATTAAACTGGCAA
  GTGGAGAAATGACAGCAGCCTCACCTGGAGTCTGTTTTTTTGGGTGGAGCAACAACTGCC
  ATGTCCACAGTAATGATGAATGCTTCTCGAGCCACGTCGCTGCCAAGGTCTTCACAGGCA
  TTTCTAAAAATGAAGAATCCTTGACTGCAGACACGCAAGTTAAAAATCACTAATTATGTT
  CTCGATCCAGGGAATATAGTTAATAACTTTTGTGTAGACTCCATACTGACCTGCTTCCCC
  ACAATTCATGGAACCCCAGGACACTATTCCTCCCACAAACCACCTCTCTGTTTCACTATC
  TAGAAACACCAGTGCCCCTCCGCTGTCACCTCTGCAGCTGTCCTTGCCCCCACTTTCTAA
  GCCAGCACAAAGCATGTTAGCAGTTACACTTCCCCTTGGATAGGGTGGCTTTTCATATGC
  AGCAGTACATTTTTGATGGTCAACAATCGGTATGTCGACATACATTAGATTTCTAGCAAG
  GAAACCCCTTTGGGTTAATCCCCATCCAGATGCAGTTCCCATGTCATCTGTCCTCATAAA
  AGATTCAGCTTCTTTTCTTGGCAGACAAAAGGCGTGATGTTGCTATTGATTACAACTTTG
  GTATTCAATTTACCAGGGCTATGTCCTTTGCCAAAGCCGCATCCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006610
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006610.2, NP_006601.2
• RefSeq Size: 2460 bp
• RefSeq ORF: 2061 bp
• Locus ID: 10747
• UniProt ID: O00187
• Cytogenetics: 1p36.22
• Domains: CCP, CUB, Tryp_SPc, EGF_CA, EGF
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: This gene encodes a member of the peptidase S1 family of serine proteases. The encoded preproprotein is proteolytically processed to generate A and B chains that heterodimerize to form the mature protease. This protease cleaves complement components C2 and C4 in order to generate C3 convertase in the lectin pathway of the complement system. The encoded protease also plays a role in the coagulation cascade through cleavage of prothrombin to form thrombin. Myocardial infarction and acute stroke patients exhibit reduced serum concentrations of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (1) is the longer transcript and encodes isoform 1 which activates complement component 4.