IRS1 (NM_005544) Human Untagged Clone

CAT#: SC124032

IRS1 (untagged)-Human insulin receptor substrate 1 (IRS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IRS1
• Synonyms: HIRS-1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124032 sequence for NM_005544 edited (data generated by NextGen Sequencing)
  ATGGCGAGCCCTCCGGAGAGCGATGGCTTCTCGGACGTGCGCAAGGTGGGCTACCTGCGC
  AAACCCAAGAGCATGCACAAACGCTTCTTCGTACTGCGCGCGGCCAGCGAGGCTGGGGGC
  CCGGCGCGCCTCGAGTACTACGAGAACGAGAAGAAGTGGCGGCACAAGTCGAGCGCCCCC
  AAACGCTCGATCCCCCTTGAGAGCTGCTTCAACATCAACAAGCGGGCTGACTCCAAGAAC
  AAGCACCTGGTGGCTCTCTACACCCGGGACGAGCACTTTGCCATCGCGGCGGACAGCGAG
  GCCGAGCAAGACAGCTGGTACCAGGCTCTCCTACAGCTGCACAACCGTGCTAAGGGCCAC
  CACGACGGAGCTGCGGCCCTCGGGGCGGGAGGTGGTGGGGGCAGCTGCAGCGGCAGCTCC
  GGCCTTGGTGAGGCTGGGGAGGACTTGAGCTACGGTGACGTGCCCCCAGGACCCGCATTC
  AAAGAGGTCTGGCAAGTGATCCTGAAGCCCAAGGGCCTGGGTCAGACAAAGAACCTGATT
  GGTATCTACCGCCTTTGCCTGACCAGCAAGACCATCAGCTTCGTGAAGCTGAACTCGGAG
  GCAGCGGCCGTGGTGCTGCAGCTGATGAACATCAGGCGCTGTGGCCACTCGGAAAACTTC
  TTCTTCATCGAGGTGGGCCGTTCTGCCGTGACGGGGCCCGGGGAGTTCTGGATGCAGGTG
  GATGACTCTGTGGTGGCCCAGAACATGCACGAGACCATCCTGGAGGCCATGCGGGCCATG
  AGTGATGAGTTCCGCCCTCGCAGCAAGAGCCAGTCCTCGTCCAACTGCTCTAACCCCATC
  AGCGTCCCCCTGCGCCGGCACCATCTCAACAATCCCCCGCCCAGCCAGGTGGGGCTGACC
  CGCCGATCACGCACTGAGAGCATCACCGCCACCTCCCCGGCCAGCATGGTGGGCGGGAAG
  CCAGGCTCCTTCCGTGTCCGCGCCTCCAGTGACGGCGAAGGCACCATGTCCCGCCCAGCC
  TCGGTGGACGGCAGCCCTGTGAGTCCCAGCACCAACAGAACCCACGCCCACCGGCATCGG
  GGCAGCGCCCGGCTGCACCCCCCGCTCAACCACAGCCGCTCCATCCCCATGCCGGCTTCC
  CGCTGCTCGCCTTCGGCCACCAGCCCGGTCAGTCTGTCGTCCAGTAGCACCAGTGGCCAT
  GGCTCCACCTCGGATTGTCTCTTCCCACGGCGATCTAGTGCTTCGGTGTCTGGTTCCCCC
  AGCGATGGCGGTTTCATCTCCTCGGATGAGTATGGCTCCAGTCCCTGCGATTTCCGGAGT
  TCCTTCCGCAGTGTCACTCCGGATTCCCTGGGCCACACCCCACCAGCCCGCGGTGAGGAG
  GAGCTAAGCAACTATATCTGCATGGGTGGCAAGGGGCCCTCCACCCTGACCGCCCCCAAC
  GGTCACTACATTTTGTCTCGGGGTGGCAATGGCCACCGCTGCACCCCAGGAACAGGCTTG
  GGCACGAGTCCAGCCTTGGCTGGGGATGAAGCAGCCAGTGCTGCAGATCTGGATAATCGG
  TTCCGAAAGAGAACTCACTCGGCAGGCACATCCCCTACCATTACCCACCAGAAGACCCCG
  TCCCAGTCCTCAGTGGCTTCCATTGAGGAGTACACAGAGATGATGCCTGCCTACCCACCA
  GGAGGTGGCAGTGGAGGCCGACTGCCGGGACACAGGCACTCCGCCTTCGTGCCCACCCGC
  TCCTACCCAGAGGAGGGTCTGGAAATGCACCCCTTGGAGCGTCGGGGGGGGCACCACCGC
  CCAGACAGCTCCACCCTCCACACGGATGATGGCTACATGCCCATGTCCCCAGGGGTGGCC
  CCAGTGCCCAGTGGCCGAAAGGGCAGTGGAGACTATATGCCCATGAGCCCCAAGAGCGTA
  TCTGCCCCACAGCAGATCATCAATCCCATCAGACGCCATCCCCAGAGAGTGGACCCCAAT
  GGCTACATGATGATGTCCCCCAGCGGTGGCTGCTCTCCTGACATTGGAGGTGGCCCCAGC
  AGCAGCAGCAGCAGCAGCAACGCCGTCCCTTCCGGGACCAGCTATGGAAAGCTGTGGACA
  AACGGGGTAGGGGGCCACCACTCTCATGTCTTGCCTCACCCCAAACCCCCAGTGGAGAGC
  AGCGGTGGTAAGCTCTTACCTTGCACAGGTGACTACATGAACATGTCACCAGTGGGGGAC
  TCCAACACCAGCAGCCCCTCCGACTGCTACTACGGCCCTGAGGACCCCCAGCACAAGCCA
  GTCCTCTCCTACTACTCATTGCCAAGATCCTTTAAGCACACCCAGCGCCCCGGGGAGCCG
  GAGGAGGGTGCCCGGCATCAGCACCTCCGCCTTTCCACTAGCTCTGGTCGCCTTCTCTAT
  GCTGCAACAGCAGATGATTCTTCCTCTTCCACCAGCAGCGACAGCCTGGGTGGGGGATAC
  TGCGGGGCTAGGCTGGAGCCCAGCCTTCCACATCCCCACCATCAGGTTCTGCAGCCCCAT
  CTGCCTCGAAAGGTGGACACAGCTGCTCAGACCAATAGCCGCCTGGCCCGGCCCACGAGG
  CTGTCCCTGGGGGATCCCAAGGCCAGCACCTTACCTCGGGCCCGAGAGCAGCAGCAGCAG
  CAGCAGCCCTTGCTGCACCCTCCAGAGCCCAAGAGCCCGGGGGAATATGTCAATATTGAA
  TTTGGGAGTGATCAGTCTGGCTACTTGTCTGGCCCGGTGGCTTTCCACAGCTCACCTTCT
  GTCAGGTGTCCATCCCAGCTCCAGCCAGCTCCCAGAGAGGAAGAGACTGGCACTGAGGAG
  TACATGAAGATGGACCTGGGGCCGGGCCGGAGGGCAGCCTGGCAGGAGAGCACTGGGGTC
  GAGATGGGCAGACTGGGCCCTGCACCTCCCGGGGCTGCTAGCATTTGCAGGCCTACCCGG
  GCAGTGCCCAGCAGCCGGGGTGACTACATGACCATGCAGATGAGTTGTCCCCGTCAGAGC
  TACGTGGACACCTCGCCAGCTGCCCCTGTAAGCTATGCTGACATGCGAACAGGCATTGCT
  GCAGAGGAGGTGAGCCTGCCCAGGGCCACCATGGCTGCTGCCTCCTCATCCTCAGCAGCC
  TCTGCTTCCCCGACTGGGCCTCAAGGGGCAGCAGAGCTGGCTGCCCACTCGTCCCTGCTG
  GGGGGCCCACAAGGACCTGGGGGCATGAGCGCCTTCACCCGGGTGAACCTCAGTCCTAAC
  CGCAACCAGAGTGCCAAAGTGATCCGTGCAGACCCACAAGGGTGCCGGCGGAGGCATAGC
  TCCGAGACTTTCTCCTCAACACCCAGTGCCACCCGGGTGGGCAACACAGTGCCCTTTGGA
  GCGGGGGCAGCAGTAGGGGGCGGTGGCGGTAGCAGCAGCAGCAGCGAGGATGTGAAACGC
  CACAGCTCTGCTTCCTTTGAGAATGTGTGGCTGAGGCCTGGGGAGCTTGGGGGAGCCCCC
  AAGGAGCCAGCCAAACTGTGTGGGGCTGCTGGGGGTTTGGAGAATGGTCTTAACTACATA
  GACCTGGATTTGGTCAAGGACTTCAAACAGTGCCCTCAGGAGTGCACCCCTGAACCGCAG
  CCTCCCCCACCCCCACCCCCTCATCAACCCCTGGGCAGCGGTGAGAGCAGCTCCACCCGC
  CGCTCAAGTGAGGATTTAAGCGCCTATGCCAGCATCAGTTTCCAGAAGCAGCCAGAGGAC
  CGTCAGTAG
  
  Clone variation with respect to NM_005544.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005544 unedited
  AGCCGTTCANAATTTGTATACGACTCACTATAGGCGGCCCGCGAATTCGCACGAGGTTTT
  TCGGAGCCTCCCTCTGCTCAGCGTTGGTGGTGGCGGTGGCAGCATGGCGAGCCCTCCGGA
  GAGCGATGGCTTCTCGGACGTGCGCAAGGTGGGCTACCTGCGCAAACCCAAGAGCATGCA
  CAAACGCTTCTTCGTACTGCGCGCGGCCAGCGAGGCTGGGGGCCCGGCGCGCCTCGAGTA
  CTACGAGAACGAGAAGAAGTGGCGGCACAAGTCGAGCGCCCCCAAACGCTCGATCCCCCT
  TGAGAGCTGCTTCAACATCAACAAGCGGGCTGACTCCAAGAACAAGCACCTGGTGGCTCT
  CTACACCCGGTACGAGCACTTTGCCATCGCGGCGGACAGCGTGGCCTAGCAAGACAGCTG
  GTACCAGGCTCTCCTACAGCTGCACAACCGTGCTAAGGGCCACCACGACGGAGCTGCGGC
  CCTCGGGGCGGGAGGTGGTGGGGGCAGCTGCAGCGGCAGAGTCCGGCCTTGGTGAGGCTG
  GGGAGGACTTGAGCTACGGTGACTTGCCCCCAGGACCCGCATTCAAAGAGGTCTGGCAAG
  TGATCCTGAAGCCCAAGGGCCTGGTTCAGACAAAGAACCTGATTGGTATCTACCGGTTTT
  GGCTGACCAGCAAGACCATCACCTTTCGGAACTTAACTTCGAGGCACCGGCCGTGGTGCC
  TCCACTGATGAACATTTGGCGCTGTGGCCACTCGGGAAAACTTTTCTTTCTCGAGGTGGG
  CCGTTCTGTCTTGACCGATCCCGGGGNATTCTTGCATCCAGGTGGGATGACTTTGTGGGG
  GCCCGTAAACTGCTGGAAACATCTTGGGGGGCCTGCTGGGCCATGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005544 unedited
  GGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAA
  CAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTCAACAG
  TATCTAGTTTATTATGAATAGAAAGACATTTCCAGAATTTACAAACGCCCACATTGTTCA
  TTCCAAAGAGAGCTTTTGAATTGAAGGAATCACCCATCTCCTTCTGTTTACAATACATGC
  AATGGAAGACTTTAATTTCCTCTTCTTATAATTTTGCTTCCATTCAATTACCAATTTTAA
  GCTGACATTTGAAAACACCCAGCCAAATATATCCTTCTTCAGTTTAATTAACATGGCCTA
  TTGTCTTACAGGAAAAAATACAAAATTAAGATTAATTCTTATAAGGAACTCAGCTCAACA
  TCAGGTTTATATGACAAGAGATAGTATCAGCAAATATAACTATACAATTGAGAACCATCT
  ATGGCACTATGATTCTTATATTATGTTTTCTGAATTACAATCTTAAAACAAGGATCATAC
  CCTATTCTACTCTTTTAAGCCTTGTTTTAAGCATTTGCTCCACTCTTTACAACAGAACAT
  TGTATACCTCCATCCCACATCCAAAAAAAAGATGTGCTGTAAGAAAAGTTACATTTTCAT
  TTAAAAAATTACACATATAAATCANAACCTATGTTAAATATTTAATACTCTCTCCACCCA
  ACGTGAACAGTTTTGTATGAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005544
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005544.1, NP_005535.1
• RefSeq Size: 5828 bp
• RefSeq ORF: 3729 bp
• Locus ID: 3667
• UniProt ID: P35568
• Cytogenetics: 2q36.3
• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Insulin signaling pathway, Neurotrophin signaling pathway, Type II diabetes mellitus
• Gene Summary: This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]