RANKL (TNFSF11) (NM_003701) Human Untagged Clone

CAT#: SC123947

TNFSF11 (untagged)-Human tumor necrosis factor (ligand) superfamily, member 11 (TNFSF11), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RANKL
• Synonyms: CD254; hRANKL2; ODF; OPGL; OPTB2; RANKL; sOdf; TNLG6B; TRANCE
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC123947 sequence for NM_003701 edited (data generated by NextGen Sequencing)
  ATGCGCCGCGCCAGCAGAGACTACACCAAGTACCTGCGTGGCTCGGAGGAGATGGGCGGC
  GGCCCCGGAGCCCCGCACGAGGGCCCCCTGCACGCCCCGCCGCCGCCTGCGCCGCACCAG
  CCCCCCGCCGCCTCCCGCTCCATGTTCGTGGCCCTCCTGGGGCTGGGGCTGGGCCAGGTT
  GTCTGCAGCGTCGCCCTGTTCTTCTATTTCAGAGCGCAGATGGATCCTAATAGAATATCA
  GAAGATGGCACTCACTGCATTTATAGAATTTTGAGACTCCATGAAAATGCAGATTTTCAA
  GACACAACTCTGGAGAGTCAAGATACAAAATTAATACCTGATTCATGTAGGAGAATTAAA
  CAGGCCTTTCAAGGAGCTGTGCAAAAGGAATTACAACATATCGTTGGATCACAGCACATC
  AGAGCAGAGAAAGCGATGGTGGATGGCTCATGGTTAGATCTGGCCAAGAGGAGCAAGCTT
  GAAGCTCAGCCTTTTGCTCATCTCACTATTAATGCCACCGACATCCCATCTGGTTCCCAT
  AAAGTGAGTCTGTCCTCTTGGTACCATGATCGGGGTTGGGCCAAGATCTCCAACATGACT
  TTTAGCAATGGAAAACTAATAGTTAATCAGGATGGCTTTTATTACCTGTATGCCAACATT
  TGCTTTCGACATCATGAAACTTCAGGAGACCTAGCTACAGAGTATCTTCAACTAATGGTG
  TACGTCACTAAAACCAGCATCAAAATCCCAAGTTCTCATACCCTGATGAAAGGAGGAAGC
  ACCAAGTATTGGTCAGGGAATTCTGAATTCCATTTTTATTCCATAAACGTTGGTGGATTT
  TTTAAGTTACGGTCTGGAGAGGAAATCAGCATCGAGGTCTCCAACCCCTCCTTACTGGAT
  CCGGATCAGGATGCAACATACTTTGGGGCTTTTAAAGTTCGAGATATAGATTGA
  
  Clone variation with respect to NM_003701.3
  126 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003701 unedited
  CATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCGCGCCCCAGG
  AGCCAAAGCCGGGCTCCAAGTCGGCGCCCCACGTCGAGGCTCCGCCGCAGCCTCCGGAGT
  TGGCCGCAGACAAGAAGGGGAGGGAGCGGGAGAGGGAGGAGAGCTCCGAAGCGAGAGGGC
  CGAGCGCCATGCGCCGCGCCAGCAGAGACTACACCAAGTACCTGCGTGGCTCGGAGGAGA
  TGGGCGGCGGCCCCGGAGCCCCGCACGAGGGCCCCCTGCACGGCCCCGCCGCCGCCTGCG
  CCGCACCAGCCCCCCGCCGCCTCCCGCTCCATGTTCGTGGCCCTCCTGGGGCTGGGGCTG
  GGCCAGGGTTGTCTGCAGCGTCGCCCTGTTCTTCTATTTCAGAGCGCAGATGGGATCCTA
  ATAGAATATCAGAAGATGGCACTCACTGCATTTATAGAATTTTGAGACTCCATGAAAATG
  CAGATTTTCAAGACACAACTCTGGAGAGTCAAGATACAAAATTAATACCTGGATCATGTA
  GGAGAATTAAACAGGCCTTTCAAGGAGCTGTGCAAAAAGAATTACAACCTATCGTTGGAT
  CACAGCACAATCGAAGCAGAAGAAAGCGATTGGTGGATGGGCTCAATGGTTTAGATCTGG
  CCAAGAGGAAGCAAGGCTTGAAACTCAGCCCCTTTGGCTCAATCTTAACTATTTAATGCC
  AACCGAAATTTCCCATCTGGGTTCCCCATAAAAGTGGAGTCTGTCCCTCTTGGTTACCCC
  ATGGATTCCGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003701 unedited
  CATGCAACTTCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGAT
  CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTGTTT
  TTTTTTTCAGGTTTTCATATAAATTTATTTTTGTACCAAAAAATTAACATTCAAAGGCAA
  TGTTTTTATTGTACAGTATAAGATAACATTTACTAAATTAAAATTGGACTTTTTTGCTGA
  TAAACCACTAATAACTTTTCTATTTTGAAAAAAAAAAAAAAAAAGGTACAATTTGCGGCA
  CTTGTGGAAAAACACCTGTTCAACAATTATATAACTACTCTTGACAGATTTCAGTGGCCC
  ATTATGTATCCTTTTTGGCAATGTCAGCTTTCTAAAGTCAATTAGTTTCTAAATTTGAAA
  GGCACCTGGTGGCCAACATCCTGCTTATTATTCAAGGCATCCATTTTAATTTTTTTGCAC
  AGGCTTGACAAGTCTGAAAAAATCTATTAACTTAAAAAGAACGAAGAAATATACTGCATT
  TGATATTAGGAAACAACATTTTTTTCCCCTCCTTAGCTGCAAGTTTTCCCCAGGGAATTT
  ACAAAGTGCACCAGTTAAATATGTCTTGTCTTTTAAAACATTAATATGTCAACGGCAAGA
  CATTTTTAAATATTTTGAATCAATACTATGGCACAAAATTTATTTTACATACTTTGCAAA
  GAAACATTACATCTGAAATATACTTTAGATATTATATAAAATACCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003701
• Insert Size: 2300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003701.2, NP_003692.1
• RefSeq Size: 2226 bp
• RefSeq ORF: 954 bp
• Locus ID: 8600
• UniProt ID: O14788
• Cytogenetics: 13q14.11
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction
• Gene Summary: This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer protein (isoform 1).