PTGIS (NM_000961) Human Untagged Clone

CAT#: SC123875

PTGIS (untagged)-Human prostaglandin I2 (prostacyclin) synthase (PTGIS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTGIS
• Synonyms: CYP8; CYP8A1; PGIS; PTGI
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC123875 sequence for NM_000961 edited (data generated by NextGen Sequencing)
  ATGGCTTGGGCCGCGCTCCTCGGCCTCCTGGCCGCACTGTTGCTGCTGCTGCTACTGAGC
  CGCCGCCGCACGCGGCGACCTGGTGAGCCTCCCCTGGACCTGGGCAGCATCCCCTGGTTG
  GGGTATGCCTTGGACTTTGGAAAAGATGCTGCCAGCTTCCTCACGAGGATGAAGGAGAAG
  CACGGTGACATCTTTACTATACTGGTTGGGGGCAGGTATGTCACCGTTCTCCTGGACCCA
  CACTCCTACGACGCGGTGGTGTGGGAGCCTCGCACCAGGCTCGACTTCCATGCCTATGCC
  ATCTTCCTCATGGAGAGGATTTTTGATGTGCAGCTTCCACATTACAGCCCCAGTGATGAA
  AAGGCCAGGATGAAACTGACTCTTCTCCACAGAGAGCTCCAGGCACTCACAGAAGCCATG
  TATACCAACCTCCATGCAGTGCTGTTGGGCGATGCTACAGAAGCAGGCAGTGGCTGGCAC
  GAGATGGGTCTCCTCGACTTCTCCTACAGCTTCCTGCTCAGAGCCGGCTACCTGACTCTT
  TACGGAATTGAGGCGCTGCCACGCACCCATGAAAGCCAGGCCCAGGACCGCGTCCACTCA
  GCTGATGTCTTCCACACCTTTCGCCAGCTCGACCGGCTGCTCCCCAAACTGGCCCGTGGC
  TCCCTGTCAGTGGGGGACAAGGACCACATGTGCAGTGTCAAAAGTCGCCTGTGGAAGCTG
  CTATCCCCAGCCAGGCTGGCCAGGCGGGCCCACCGGAGCAAATGGCTGGAGAGTTACCTG
  CTGCACCTGGAGGAGATGGGTGTGTCAGAGGAGATGCAGGCACGGGCCCTGGTGCTGCAG
  CTGTGGGCCACACAGGGGAATATGGGTCCCGCTGCCTTCTGGCTCCTGCTCTTCCTTCTC
  AAGAATCCTGAAGCCCTGGCTGCTGTCCGCGGAGAGCTCGAGAGTATCCTTTGGCAAGCG
  GAGCAGCCTGTCTCGCAGACGACCACTCTCCCACAGAAGGTTCTAGACAGCACACCTGTG
  CTTGATAGCGTGCTGAGTGAGAGCCTCAGGCTTACAGCTGCCCCCTTCATCACCCGCGAG
  GTTGTGGTGGACCTGGCCATGCCCATGGCAGACGGGCGAGAATTCAACCTGCGACGTGGT
  GACCGCCTCCTCCTCTTCCCCTTCCTGAGCCCCCAGAGAGACCCAGAAATCTACACAGAC
  CCAGAGGTATTTAAATACAACCGATTCCTGAACCCTGACGGATCAGAGAAGAAAGACTTT
  TACAAGGATGGGAAACGGCTGAAGAATTACAACATGCCCTGGGGGGCGGGGCACAATCAC
  TGCCTGGGGAGGAGTTATGCGGTCAACAGCATCAAACAATTTGTGTTCCTTGTGCTGGTG
  CACTTGGACTTGGAGCTGATCAACGCAGATGTGGAGATCCCTGAGTTTGACCTCAGCAGG
  TACGGCTTCGGTCTGATGCAGCCGGAACACGACGTGCCCGTCCGCTACCGCATCCGCCCA
  TGA
  
  Clone variation with respect to NM_000961.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000961 unedited
  TCAGAATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCCCGCCAGC
  CCCGCCAGCCCCGCGATGGCTTGGGCCGCGCTCCTCGGCCTCCTGGCCGCACTGTTGCTG
  CTGCTGCTACTGAGCCGCCGCCGCACGCGGCGACCTGGTGAGCCTCCCCTGGACCTGGGC
  AGCATCCCCTGGTTGGGGTATGCCTTGGACTTTGGAAAAGATGCTGCCAGCTTCCTCACG
  AGGATGAAGGAGAAGCACGGTGACATCTTTACTATACTGGTTGGGGGCAGGTATGTCACC
  GTTCTCCTGGACCCACACTCCTACGACGCGGTGGTGTGGGAGCCTCGCACCAGGCTCGAC
  TTCCATGCCTATGCCATCTTCCTCATGGAGAGGATTTTTGATGTGCAGCTTCCACATTAC
  AGCCCCAGTGATGAAAAGGCCAGGATGAAACTGACTCTTCTCCACAGAGAGCTCCAGGCA
  CTCACAGAAGCCATGTATACCAACCTCCATGCAGTGCTGTTGGGCGATGCTACAGAAGCA
  GGCAGTGGCTGGCACGAGATGGGTCTCCTCGACTTCTCCTACAGCTTCCTGCTCAGAGCC
  GGCTACCTGACTCTTTACGGAATTGAGGCGCTGCCACGCACCCATGAAAGCCAGGCCCAG
  GACCGCGTCCACTCAGCTGATGTCTTCCACACCTTTCGCCAGCTCGACCGGCTGCTCCCC
  AAACTGGCCCGTGGCTCCCTGTCAGTGGGGGACAAGACCACATGTGCAGTGTCAAAAGTC
  GCCTGTGGAAGCTGCTATCCCCAGCCAGGCTGCCAGGCGGGCCCACGGAACCAAATGGTG
  GAGAAGTACCCGCTGCACCTGGAGGAAATGGGGGTGTCAAAAGAGATGCAAGCACGGGCC
  CTGGTGCTGCACCTTGGGAGCACAAGGGGAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000961 unedited
  GCACGTCGTGTTCCGGCTGCATCAGACCGAAGCCGTACCTGCTGAGGTCAAACTCAGGGA
  TCTCCACATCTGCGTTGATCAGCTCCAAGTCCAAGTGCACCAGCACAAGGAACACAAATT
  GTTTGATGCTGTTGACCGCATAACTCCTCCCCAGGCAGTGATTGTGCCCCGCCCCCCAGG
  GCATGTTGTAATTCTTCAGCCGTTTCCCATCCTTGTAAAAGTCTTTCTTCTCTGATCCGT
  CAGGGTTCAGGAATCGGTTGTATTTAAATACCTCTGGGTCTGTGTAGATTTCTGGGTCTC
  TCTGGGGGCTCAGGAAGGGGAAGAGGAGGAGGCGGTCACCACGTCGCAGGTTGAATTCTC
  GCCCGTCTGCCATGGGCATGGCCAGGTCCACCACAACCTCGCGGGTGATGAAGGGGGCAG
  CTGTAAGCCTGAGGCTCTCACTCAGCACGCTATCAAGCACAGGTGTGCTGTCTAGAACCT
  TCTGTGGGAGAGTGGTCGTCTGCGAGACAGGCTGCTCCGCTTGCCAAAGGATACTCTCGA
  GCTCTCCGCGGACAGCAGCCAGGGCTTCAGGATTCTTGAGAAGGAAGAGCAGGAGCCAGA
  AAGCAGCGGGACCCATATTCCCCTGTGTGGCCCACAGCTGCAGCACCAGGGCCCGTGCCT
  GCATCTCCTCTGACACACCCATCTCCTCCAGGTGCAGCAGGTAACTCTCCAGCCATTTGC
  TCCGGTGGGCCCGCCTGGCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000961
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000961.3, NP_000952.1
• RefSeq Size: 5603 bp
• RefSeq ORF: 1503 bp
• Locus ID: 5740
• UniProt ID: Q16647
• Cytogenetics: 20q13.13
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to prostacyclin (prostaglandin I2), a potent vasodilator and inhibitor of platelet aggregation. An imbalance of prostacyclin and its physiological antagonist thromboxane A2 contribute to the development of myocardial infarction, stroke, and atherosclerosis. [provided by RefSeq, Jul 2008]