FA2H (NM_024306) Human Untagged Clone

CAT#: SC122956

FA2H (untagged)-Human fatty acid 2-hydroxylase (FA2H)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FA2H
• Synonyms: FAAH; FAH1; FAXDC1; SCS7; SPG35
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_024306 edited
  AGCAGACGCCGGGCTGCGCGCTGGGAGCTGCGGTCTCCGGCCATGGCCCCCGCTCCGCCC
  CCCGCCGCCTCCTTCTCGCCCTCCGAGGTCCAGCGGCGCCTGGCGGCCGGCGCGTGCTGG
  GTCCGCCGCGGGGCCCGCCTCTACGACCTCTCCAGCTTCGTGCGGCACCACCCGGGGGGC
  GAGCAGCTGCTGCGGGCCAGGGCGGGCCAGGACATCAGCGCCGACCTGGACGGGCCGCCG
  CACAGGCACTCGGCCAACGCGCGCCGCTGGCTGGAGCAGTACTACGTGGGAGAGCTCCGC
  GGGGAGCAGCAGGGCTCCATGGAGAACGAGCCTGTAGCCCTTGAGGAAACTCAGAAGACA
  GATCCTGCTATGGAACCACGGTTCAAAGTGGTGGATTGGGACAAGGACCTGGTGGACTGG
  CGAAAGCCTCTCCTGTGGCAGGTGGGCCACTTGGGAGAGAAGTACGATGAGTGGGTTCAC
  CAGCCGGTGACCAGGCCCATCCGCCTCTTCCACTCAGACCTCATTGAGGGCCTCTCTAAG
  ACTGTCTGGTACAGTGTCCCCATCATCTGGGTGCCCCTGGTGCTGTATCTCAGCTGGTCC
  TACTACCGAACCTTTGCCCAGGGCAACGTCCGACTCTTCACGTCATTTACAACAGAGTAC
  ACGGTGGCAGTGCCCAAGTCCATGTTCCCCGGGCTCTTCATGCTGGGGACATTCCTCTGG
  AGCCTCATCGAGTACCTCATCCACCGCTTCCTGTTCCACATGAAGCCCCCCAGCGACAGC
  TATTACCTCATCATGCTGCACTTCGTCATGCACGGCCAGCACCACAAGGCACCCTTCGAC
  GGCTCCCGCCTGGTCTTCCCCCCTGTGCCAGCCTCCCTGGTGATCGGCGTCTTCTACTTG
  TGCATGCAGCTCATCCTGCCTGAGGCAGTAGGGGGCACTGTGTTTGCGGGGGGCCTCCTG
  GGCTACGTCCTCTATGACATGACCCATTACTACCTGCACTTTGGCTCGCCGCACAAGGGC
  TCCTACCTGTACAGCCTGAAGGCCCACCACGTCAAGCACCACTTTGCACATCAGAAGTCA
  GGATTTGGTATCAGCACTAAATTGTGGGATTACTGTTTCCACACCCTCACTCCAGAGAAA
  CCCCACCTGAAGACGCAGTGACAACTCCCACCCCCTCCGTCCTGCCCTCAGCCCGGCCCT
  GGCCCCTTCCCGACCCCCACCCGCCATTCAGACCCCATTAAGAAGGTTGGCTTGGCCAGG
  CAGGATGGGCTGTGTCCGGCCCTGCAGCCTAGTGGAAGGTGCTGAGGGGGCCCTGAGGCA
  GGACCGCCCTCCTGACCCCTGGTAGGAGGGTCACATCCACTTGGTGGCCAGGTGGCCCTT
  GGTGACCCACTTCTTCCTGGAGCGTCCCTGCCTAGAGCTCAGCCCACAGGACTGCTTCAG
  GCCGTGGCCACAGGTAGCAGCCGCAAGGGGAAATGAAGAAAACTGAGCCCTCGTGGCCAC
  CTGTGTCACCCTTGTGCCTTAGCCTCATGGGCTGCCTAGGAGCTGCCTGCACGGCACAGC
  TCGCTTTCACAGTCAGAAGTGGGTCTGTGGGATCTGTGGTCCCTGTCCTCCCTGCTGTCC
  CTTCTGGGGAGGCTTTGGTGGCTCTGAGGTGGACAAAGAGCTCTCGCAAGAAGAGACAGC
  GTGATGCCTCCCACAGTCCACCCCAGACCCTGGGGCAGCCCCTCTGGCCCTGCCAGCTGC
  CTGCGTCGTTGGGCCCAGGGTGGCTGGCAGGAGTCCCAGCTGCTTGCTTTAGGACCTCGC
  AGCTTTTCTTGCCGTCCCTCCCCTGCCTCCAGAATCACAGCCCTTCTCCCCAAGGGAGGC
  TGAGGAGGCTTCTCCACCAGTGGCAGCCCCACCCCGTCCCTGGCCATTCTTGGCCTCCAC
  CCCGCTCAGGCCCCTACTCGGGCGCTCCCAGAAGGAGCCACCTCTCAGTGCCTCACCTCC
  CCCTGCCTCCCAGCCTCCGCAGATGAGGTTCCTGCCCCTTCCTCCTCGTAACCAAAACCC
  TCACTGCTCCCAGGACGGTCTTATTTATAAACCAGATACATGTTCTTAGTCTGGTCCCAG
  ACCAAGGAGCTGGTCAGACGGCCCTTTCTAATCCTACATGTTGAGCTTATGTAAAAAATG
  TTGTTTCCTCCTGTTTTTGGTTCCTTTCTTACCCACAAACCATTACTACTTGAAACTTAA
  AAAACTCGCCAAGTGTAAAGGCTAAAGAGAAGCAGTTTGACGGACCTTGTGATTTGTACT
  GTTTGCTGCGGAGCTATTTAAAGATTTTGGAATAAATATACAAAACTACGGTTGTGAAAT
  AAAAACTTAAATTGTATATTTTGAAAAATAAAACACTGAAAAGAAACCAAAAAAAAAAAA
  AAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024306 unedited
  NAACGTCAGAATTGTATACGACTCATATAGGCGGCCGCNGAATTCGCACGAGGAGCAGAC
  GCCGGGCTGCGCGCTGGGAGCTGCGGTCTCCGGCCATGGCCCCCGCTCCGCCCCCCGCCG
  CCTCCTTCTCGCCCTCCGAGGTCAAGCGGCGCCTGGCGGCCGGCGCGTGCTGGGTCCGCC
  GCGGGGCCCGCCTCTACGACCTCTCCAGCTTCGTGCGGCACCACCCGGGGGGCGAGCAGC
  TGCTGCGGGCCAGGGCGGGCCAGGACATCAGCGCCGACCTGGACGGGCCGCCGCACAGGC
  ACTCGGCCAACGCGCGCCGCTGGCTGGAGCAGTACTACGTGGGAGAGCTCCGCGGGGAGC
  AGCAGGGCTCCATGGAGAACGAGCCTGTAGCCCTTGAGGAAACTCAGAAGACAGATCCTG
  CTATGGAACCACGGTTCAAAGTGGTGGATTGGGACAAGGACCTGGTGGACTGGCGAAAGC
  CTCTCCTGTGGCAGGTGGGCCACTTGGGAGAGAAGTACGATGAGTGGGTTCACCAGCCGG
  TGACCAGGCCCATCCGCCTCTTCCACTCAGACCTCATTGAGGGCCTCTCTAAGACTGTCT
  GGTACAGTGTCCCCATCATCTGGGTGCCCCTGGTGCTGTATCTCAGCTGGTCCTACTACC
  GAACCTTTGCCCAGGGCAACGTCCGACTCTTCACGTCATTTACAACAGAGTACACGGTGG
  CAGTGCCCAAGTCCATGTTCCCCGGGCTCTTCATGCTGGGGACATTCCTCTGGAGCCTCA
  TCGAGTACCTCATCCACCGCTTCCTGNTCCACATGAAGCCCCCCAGCGACAGCTATTACC
  TCATCATGCTGCACTTCGTCATGCACGGCCAGCACCACAAGGCACCCTTNCGACGCTCCC
  GNCTGGTCTTCCCCCCTGTC
  
• Restriction Sites: Please inquire
• ACCN: NM_024306
• Insert Size: 2416 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024306.2, NP_077282.2
• RefSeq Size: 2419 bp
• RefSeq ORF: 1119 bp
• Locus ID: 79152
• UniProt ID: Q7L5A8
• Cytogenetics: 16q23.1
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a protein that catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Sphingolipids play roles in many cellular processes and their structural diversity arises from modification of the hydrophobic ceramide moiety, such as by 2-hydroxylation of the N-acyl chain, and the existence of many different head groups. Mutations in this gene have been associated with leukodystrophy dysmyelinating with spastic paraparesis with or without dystonia.[provided by RefSeq, Mar 2010]