CCR4 NOT transcription complex subunit 3 (CNOT3) (NM_014516) Human Untagged Clone

CAT#: SC122815

CNOT3 (untagged)-Human CCR4-NOT transcription complex, subunit 3 (CNOT3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCR4 NOT transcription complex subunit 3
• Synonyms: IDDSADF; LENG2; NOT3; NOT3H
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014516 edited
  GGACCGGGCCCGGTCAGCTTCCGCGGAGCCATTGGCAGACGCCGTGGCCTCCCTTGAGCC
  CCGACCCCCGTCGTCAGAACAACCCCGGGCCCACTCCCCCAACCCCACTTCCGCTTCGCG
  CCGCTATCGCGATAGCGCCCGGGCCCGGGGCGCGAGAAAAAGGCGGCGGGCGCTCGCCTC
  CCCCGCCTGTCGCGATACGCTCCTCAGCGGCGGCGCCAGCTCCTGTGCGTCCGTCTCCAA
  GAGAGTATGAAGAGAGTGCGTCTGTAGGGCAGGGAAGATGGCGGACAAGCGCAAACTCCA
  AGGTGAGATTGATCGCTGCCTCAAGAAGGTGTCCGAGGGCGTGGAGCAGTTTGAAGATAT
  TTGGCAGAAGCTCCACAATGCAGCCAACGCGAACCAGAAAGAAAAGTATGAGGCTGACCT
  AAAGAAGGAGATTAAGAAGCTACAACGGCTGAGGGACCAAATCAAGACATGGGTAGCGTC
  CAACGAGATCAAGGACAAGAGGCAGCTTATAGACAACCGCAAGCTCATTGAGACGCAAAT
  GGAACGGTTCAAAGTTGTGGAACGAGAGACCAAAACCAAAGCTTACAGCAAAGAGGGCCT
  GGGCCTGGCCCAGAAGGTAGATCCTGCCCAGAAGGAGAAGGAAGAGGTTGGCCAGTGGCT
  CACGAATACCATCGACACGCTCAACATGCAGGTGGACCAGTTTGAGAGTGAAGTGGAGTC
  ACTGTCAGTGCAGACACGCAAGAAGAAGGGCGACAAGGATAAGCAGGACCGGATTGAGGG
  CTTGAAGCGGCACATCGAGAAGCACCGCTACCACGTGCGCATGCTAGAGACCATCCTGCG
  CATGCTGGACAATGACTCCATCCTCGTTGACGCCATCCGCAAGATCAAGGACGACGTTGA
  GTACTATGTTGACTCATCCCAGGACCCCGACTTCGAGGAGAACGAGTTTCTCTACGATGA
  CCTGGACCTCGAGGACATTCCACAGGCGCTGGTCGCCACCTCCCCCCCCAGCCACAGCCA
  CATGGAGGATGAGATCTTCAACCAGTCCAGCAGCACGCCCACCTCAACCACCTCCAGCTC
  TCCCATCCCGCCCAGCCCAGCCAACTGTACCACGGAAAACTCTGAAGATGATAAGAAGAG
  GGGACGTTCCACAGACAGTGAAGTCAGCCAGTCTCCAGCCAAAAACGGCTCCAAGCCTGT
  CCACAGCAACCAGCACCCTCAGTCCCCAGCTGTGCCGCCCACCTACCCCTCCGGCCCCCC
  GCCTGCTGCCTCTGCCTTGAGCACCACTCCTGGCAACAATGGGGTCCCCGCCCCCGCAGC
  ACCCCCAAGTGCCCTGGGCCCCAAGGCCAGTCCAGCTCCCAGCCACAACTCGGGCACCCC
  TGCTCCCTATGCCCAGGCGGTGGCCCCACCAGCTCCCAGTGGGCCCAGCACGACCCAGCC
  CCGGCCCCCCAGCGTCCAGCCTAGCGGAGGCGGAGGCGGCGGCAGCGGAGGCGGAGGGAG
  CAGCAGCAGTAGTAACAGCAGTGCCGGTGGAGGGGCTGGCAAGCAGAATGGCGCCACCAG
  TTACAGCTCAGTTGTGGCAGACAGCCCGGCAGAGGTGGCTTTGAGCAGCAGTGGGGGCAA
  CAATGCCAGCAGCCAGGCCTTGGGCCCCCCTTCCGGCCCCCACAACCCACCTCCCAGCAC
  CTCGAAGGAACCCAGTGCGGCAGCCCCAACGGGGGCTGGGGGCGTGGCCCCAGGCTCAGG
  GAACAACTCAGGGGGACCCAGCCTCCTGGTGCCACTGCCTGTGAATCCTCCCAGCTCCCC
  AACGCCCAGCTTCAGTGATGCCAAGGCAGCCGGTGCCCTGCTCAATGGGCCTCCACAGTT
  CAGCACCGCCCCAGAAATCAAGGCCCCTGAGCCTCTGAGCTCCTTGAAGTCCATGGCGGA
  ACGGGCAGCCATCAGCTCTGGCATTGAGGACCCTGTGCCAACGCTGCACCTGACCGAGCG
  AGACATCATCCTGAGCAGTACATCAGCACCTCCGGCCTCAGCCCAGCCGCCCCTGCAGCT
  GTCAGAGGTGAACATACCGCTGTCGCTGGGTGTCTGTCCACTGGGCCCTGTGCCCCTCAC
  CAAGGAGCAGCTCTATCAGCAGGCCATGGAAGAGGCCGCCTGGCACCACATGCCTCACCC
  CTCTGACTCTGAGCGTATTCGGCAGTACCTCCCCCGGAACCCCTGTCCGACGCCCCCCTA
  CCACCACCAGATGCCACCCCCACACTCGGACACTGTGGAATTCTACCAGCGCCTGTCGAC
  CGAGACACTCTTCTTCATCTTCTACTATCTGGAGGGCACTAAGGCACAGTATCTGGCAGC
  CAAGGCCCTAAAGAAGCAGTCATGGCGATTCCACACCAAGTACATGATGTGGTTCCAGAG
  GCACGAGGAGCCCAAGACCATCACTGACGAGTTTGAGCAGGGCACCTACATCTACTTTGA
  CTACGAGAAGTGGGGCCAGCGGAAGAAGGAAGGCTTCACCTTTGAGTACCGCTACCTGGA
  GGACCGGGACCTCCAGTGACACCGGCCCCTCCCTCTACCCACCCCCTTCCCCTTGCATGC
  TGATCCCCCTGCCCAGGTGAGGGCCCTGCCCTGGAAGACTGGAGGGAGGCCCCAAGCCAC
  GGGGCATCCCCCTCTCCCAGGAAGCAGGGAGGGGGCCGGGAGGTTTTCCTCTCAGCCCCA
  CCCTGGGGGCCCGGGGGCGAGGGCTGCCCCCTCCTCCCCTCCCCAGTGAGGGACATTTTT
  TGGTAAACCTATTTTCATTTTGGAAAATATTTATGAATAAATAGTTTTATATGAAAAAAA
  AAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014516 unedited
  CGGGTTCGGATTTGTATACGACTCATATAGGCGGCNCGCGATTCCCGGGATATCGTCGAC
  CCACGCGTCCGGGACCGGGCCCGGTCAGCTTCCGCGGAGCCATTGGCAGACGCCGTGGCC
  TCCCTTGAGCCCCGACCCCCGTCGTCAGAACAACCCCGGGCCCACTCCCCCAACCCCACT
  TCCGCTTCGCGCCGCTATCGCGATAGCGCCCGGGCCCGGGGCGCGAGAAAAAGGCGGCGG
  GCGCTCGCCTCCCCCGCCTGTCGCGATACGCTCCTCAGCGGCGGCGCCAGCTCCTGTGCG
  TCCGTCTCCAAGAGAGTATGAAGAGAGTGCGTCTGTAGGGCAGGGAAGATGGCGGACAAG
  CGCAAACTCCAAGGTGAGATTGATCGCTGCCTCAAGAAGGTGTCCGAGGGCGTGGAGCAG
  TTTGAAGATATTTGGCAGAAGCTCCACAATGCAGCCAACGCGAACCAGAAAGAAAAGTAT
  GAGGCTGACCTAAAGAAGGAGATTAAGAAGCTACAACGGCTGAGGGACCAAATCAAGACA
  TGGGTAGCGTCCAACGAGATCAAGGACAAGAGGCAGCTTATAGACCAACCGCAGCTCATT
  GAGACGCANATGGAACGGTTCANAGTTGTGGAACGAGAGACCAAAACCAAAGCTTACAGC
  ANAGAGGGCCTGGGCCTGGCCCAGAAGGTAGATCCTGCCCAGAAGGAGAANGAAGAGGTT
  GGCCAGTGGCTCACGAATACCATCGACACGCTCAACATGCAGGTGGACCAGTTTGAGAGT
  GAAGTGGAGTCACTGTCAGTGCAGACACGCAAGAAGAGGGGCGACAGGGATAGCAGACCC
  GGATGAGGNCNTTGAGCGCACATCGAGAAGCACGCTACCACGTGCGCATGCTAGAGACAT
  CCTGCGCATGCTGGAN
  
• Restriction Sites: Please inquire
• ACCN: NM_014516
• Insert Size: 2831 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014516.2, NP_055331.1
• RefSeq Size: 2855 bp
• RefSeq ORF: 2262 bp
• Locus ID: 4849
• UniProt ID: O75175
• Cytogenetics: 19q13.42
• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation
• Gene Summary: Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity.[UniProtKB/Swiss-Prot Function]