SNAIL (SNAI1) (NM_005985) Human Untagged Clone

CAT#: SC122733

SNAI1 (untagged)-Human snail homolog 1 (Drosophila) (SNAI1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SNAIL
• Synonyms: dJ710H13.1; SLUGH2; SNA; SNAH; SNAIL; SNAIL1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005985 edited
  GGCCTAGCGAGTGGTTCTTCTGCGCTACTGCTGCGCGAATCGGCGACCCCAGTGCCTCGA
  CCACTATGCCGCGCTCTTTCCTCGTCAGGAAGCCCTCCGACCCCAATCGGAAGCCTAACT
  ACAGCGAGCTGCAGGACTCTAATCCAGAGTTTACCTTCCAGCAGCCCTACGACCAGGCCC
  ACCTGCTGGCAGCCATCCCACCTCCGGAGATCCTCAACCCCACCGCCTCGCTGCCAATGC
  TCATCTGGGACTCTGTCCTGGCGCCCCAAGCCCAGCCAATTGCCTGGGCCTCCCTTCGGC
  TCCAGGAGAGTCCCAGGGTGGCAGAGCTGACCTCCCTGTCAGATGAGGACAGTGGGAAAG
  GCTCCCAGCCCCCCAGCCCACCCTCACCGGCTCCTTCGTCCTTCTCCTCTACTTCAGTCT
  CTTCCTTGGAGGCCGAGGCCTATGCTGCCTTCCCAGGCTTGGGCCAAGTGCCCAAGCAGC
  TGGCCCAGCTCTCTGAGGCCAAGGATCTCCAGGCTCGAAAGGCCTTCAACTGCAAATACT
  GCAACAAGGAATACCTCAGCCTGGGTGCCCTCAAGATGCACATCCGAAGCCACACGCTGC
  CCTGCGTCTGCGGAACCTGCGGGAAGGCCTTCTCTAGGCCCTGGCTGCTACAAGGCCATG
  TCCGGACCCACACTGGCGAGAAGCCCTTCTCCTGTCCCCACTGCAGCCGTGCCTTCGCTG
  ACCGCTCCAACCTGCGGGCCCACCTCCAGACCCACTCAGATGTCAAGAAGTACCAGTGCC
  AGGCGTGTGCTCGGACCTTCTCCCGAATGTCCCTGCTCCACAAGCACCAAGAGTCCGGCT
  GCTCAGGATGTCCCCGCTGACCCTCGAGGCTCCCTCTTCCTCTCCATACCTGCCCCTGCC
  TGACAGCCTTCCCCAGCTCCAGCAGGAAGGACCCCACATCCTTCTCACTGCCATGGAATT
  CCCTCCTGAGTGCCCCACTTCTGGCCACATCAGCCCCACAGGACTTTGATGAAGACCATT
  TTCTGGTTCTGTGTCCTCTGCCTGGGCTCTGGAAGAGGCCTTCCCATGGCCATTTCTGTG
  GAGGGAGGGCAGCTGGCCCCCAGCCCTGGGGGATTCCTGAGCTGGCCTGTCTGCGTGGGT
  TTTTGTATCCAGAGCTGTTTGGATACAGCTGCTTTGAGCTACAGGACAAAGGCTGACAGA
  CTCACTGGGAAGCTCCCACCCCACTCAGGGGACCCCACTCCCCTCACACACACCCCCCCA
  CAAGGAACCCTCAGGCCACCCTCCACGAGGTGTGACTAACTATGCAATAATCCACCCCCA
  GGTGCAGCCCCAGGGCCTGCGGAGGCGGTGGCAGACTAGAGTCTGAGATGCCCCGAGCCC
  AGGCAGCTATTTCAGCCTCCTGTTTGGTGGGGTGGCACCTGTTTCCCGGGCAATTTAACA
  ATGTCTGAAAAGGGACTGTGAGTAATGGCTGTCACTTGTCGGGGGCCCAAGTGGGGTGCT
  CTGGTCTGACCGATGTGTCTCCCAGAACTATTCTGGGGGCCCGACAGGTGGGCCTGGGAG
  GAAGATGTTTACATTTTTAAAGGTACACTGGTATTTATATTTCAAACATTTTGTATCAAG
  GAAACGTTTTGTATAGTTATATGTACAGTTTATTGATATTCAATAAAGCAGTTAATTTAT
  ATATTAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005985 unedited
  NGTTCGAATTTTGTAATACGACTCACTATAGGGCGGGCCGCGATTCANATCTGGTACCGG
  TCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGGCCTAGCGAGTGGTTCTTCTGC
  GCTACTGCTGCGCGAATCGGCGACCCCAGTGCCTCGACCACTATGCCGCGCTCTTTCCTC
  GTCAGGAAGCCCTCCGACCCCAATCGGAAGCCTAACTACAGCGAGCTGCAGGACTCTAAT
  CCAGAGTTTACCTTCCAGCAGCCCTACGACCAGGCCCACCTGCTGGCAGCCATCCCACCT
  CCGGAGATCCTCAACCCCACCGCCTCGCTGCCAATGCTCATCTGGGACTCTGTCCTGGCG
  CCCCAAGCCCAGCCAATTGCCTGGGCCTCCCTTCGGCTCCAGGAGAGTCCCAGGGTGGCA
  GAGCTGACCTCCCTGTCAGATGAGGACAGTGGGAAAGGCTCCCAGCCCCCCAGCCCACCC
  TCACCGGCTCCTTCGTCCTTCTCCTCTACTTCAGTCTCTTCCTTGGAGGCCGAGGCCTAT
  GCTGCCTTCCCAGGCTTGGGCCAAGTGCCCAAGCAGCTGGCCCAGCTCTCTGAGGCCAAG
  GATCTCCAGGCTCGAAAGGCCTTCAACTGCAAATACTGCAACAAGGAATACCTCAGCCTG
  GGTGCCCTCAAGATGCACATCCGAAGCCACACGCTGCCCTGCGTCTGCGGAACCTGCGGG
  AAGGCCTTCTCTAGGCCCTGGCTGCTACAAGGCCATGTCCGGACCCACACTGGCGAGAAG
  CCCTTCTCCTGTCCCCACTGCAGCCGTGCCTTCGCTGACCGCTCCAACCTGCGGGCCCAC
  CTNCAGACCACTCAGATGTCAAGAGTACCAGTGCCAGGCGTGTGCTCGGACCTCTNCCGA
  TGTCCTGCTCACAGCACCAGAGTCCGCTGCTCAGGAGTCCCGA
  
• Restriction Sites: Please inquire
• ACCN: NM_005985
• Insert Size: 1700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005985.2, NP_005976.2
• RefSeq Size: 1708 bp
• RefSeq ORF: 795 bp
• Locus ID: 6615
• UniProt ID: O95863
• Cytogenetics: 20q13.13
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction
• Gene Summary: The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]