PI3 (NM_002638) Human Untagged Clone

CAT#: SC122631

PI3 (untagged)-Human peptidase inhibitor 3, skin-derived (PI3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PI3
• Synonyms: cementoin; ESI; SKALP; WAP3; WFDC14
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002638 edited
  GGGGGCCAAACACCTTCCTGACACCATGAGGGCCAGCAGCTTCTTGATCGTGGTGGTGTT
  CCTCATCGCTGGGACGCTGGTTCTAGAGGCAGCTGTCACGGGAGTTCCTGTTAAAGGTCA
  AGACACTGTCAAAGGCCGTGTTCCATTCAATGGACAAGATCCCGTTAAAGGACAAGTTTC
  AGTTAAAGGTCAAGATAAAGTCAAAGCGCAAGAGCCAGTCAAAGGTCCAGTCTCCACTAA
  GCCTGGCTCCTGCCCCATTATCTTGATCCGGTGCGCCATGTTGAATCCCCCTAACCGCTG
  CTTGAAAGATACTGACTGCCCAGGAATCAAGAAGTGCTGTGAAGGCTCTTGCGGGATGGC
  CTGTTTCGTTCCCCAGTGAGAGGGAGCCGGTCCTTGCTGCACCTGTGCCGTCCCCAGAGC
  TACAGGCTCCATCTGGTCCTAAGTCCCTGCTGCCCTTCCCCTTCCCACACTGTCCATTCT
  TCCTCCCATTCAGGATGCCCACGGCTGGAGCTGCCTCTCTCATCCACTTTCCAATAAAGA
  GTTCCTTCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002638 unedited
  AAAAGGTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCCATTACGGCCGG
  GGGCCAAACACCTTCCTGACACCATGAGGGCCAGCAGCTTCTTGATCGTGGTGGTGTTCC
  TCATCGCTGGGACGCTGGTTCTAGAGGCAGCTGTCACGGGAGTTCCTGTTAAAGGTCAAG
  ACACTGTCAAAGGCCGTGTTCCATTCAATGGACAAGATCCCGTTAAAGGACAAGTTTCAG
  TTAAAGGTCAAGATAAAGTCAAAGCGCAAGAGCCAGTCAAAGGTCCAGTCTCCACTAAGC
  CTGGCTCCTGCCCCATTATCTTGATCCGGTGCGCCATGTTGAATCCCCCTAACCGCTGCT
  TGAAAGATACTGACTGCCCAGGAATCAAGAAGTGCTGTGAAGGCTCTTGCGGGATGGCCT
  GTTTCGTTCCCCAGTGAGAGGGAGCCGGTCCTTGCTGCACCTGTGCCGTCCCCAGAGCTA
  CAGGCTCCATCTGGTCCTAAGTCCCTGCTGCCCTTCCCCTTCCCACACTGTCCATTCTTC
  CTCCCATTCAGGATGCCCACGGCTGGAGCTGCCTCTCTCATCCACTTTCCAATAAAGAGT
  TCCTTCTGCAAAAAAAAAANNNAAAAAAAAAAAAAAAAACCATGTCGGCCCGCCTCGGCC
  CTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATC
  CCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCA
  GCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATAT
  ATGGGTGGAGGGGGGGGGGGTTATGGANAAGGGGCANTTTGGGNNAACAANCTTTAGGGN
  CCGCGGGGTTTATTGGAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_002638
• Insert Size: 582 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002638.2, NP_002629.1
• RefSeq Size: 623 bp
• RefSeq ORF: 354 bp
• Locus ID: 5266
• UniProt ID: P19957
• Cytogenetics: 20q13.12
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. [provided by RefSeq, Oct 2014]