Cytokeratin 10 (KRT10) (NM_000421) Human Untagged Clone

CAT#: SC122561

KRT10 (untagged)-Human keratin 10 (KRT10)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cytokeratin 10
• Synonyms: BCIE; BIE; CK10; EHK; K10; KPP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000421 edited
  CAGCATCACCATGTCTGTTCGATACAGCTCAAGCAAGCACTACTCTTCCTCCCGCAGTGG
  AGGAGGAGGAGGAGGAGGAGGATGTGGAGGAGGAGGAGGAGTGTCATCCCTAAGAATTTC
  TAGCAGCAAAGGCTCCCTTGGTGGAGGATTTAGCTCAGGGGGGTTCAGTGGTGGCTCTTT
  TAGCCGTGGGAGCTCTGGTGGGGGCTGCTTTGGGGGCTCATCAGGTGGCTATGGAGGATT
  AGGAGGTTTTGGTGGAGGTAGCTTTCGTGGAAGCTATGGAAGTAGCAGCTTTGGTGGGAG
  TTATGGAGGCAGCTTTGGAGGGGGCAGTTTCGGAGGTGGCAGCTTTGGTGGGGGCAGCTT
  TGGTGGAGGCGGCTTTGGTGGAGGCGGCTTTGGAGGAGGCTTTGGTGGTGGATTTGGAGG
  AGATGGTGGCCTTCTCTCTGGAAATGAAAAAGTAACCATGCAGAATCTGAATGACCGCCT
  GGCTTCCTACTTGGACAAAGTTCGGGCTCTGGAAGAATCAAACTATGAGCTGGAAGGCAA
  AATCAAGGAGTGGTATGAAAAGCATGGCAACTCACATCAGGGGGAGCCTCGTGACTACAG
  CAAATACTACAAAACCATCGATGACCTTAAAAATCAGATTCTCAACCTAACAACTGATAA
  TGCCAACATCCTGCTTCAGATCGACAATGCCAGGCTGGCAGCTGATGACTTCAGGCTGAA
  GTATGAGAATGAGGTAGCTCTGCGCCAGAGCGTGGAGGCTGACATCAACGGCCTGCGTAG
  GGTGCTGGATGAGCTGACCCTGACCAAGGCTGACCTGGAGATGCAAATTGAGAGCCTGAC
  TGAAGAGCTGGCCTATCTGAAGAAGAACCACGAGGAGGAAATGAAAGACCTTCGAAATGT
  GTCCACTGGTGATGTGAATGTGGAAATGAATGCTGCCCCGGGTGTTGATCTGACTCAACT
  TCTGAATAACATGAGAAGCCAATATGAACAACTTGCTGAACAAAACCGCAAAGATGCTGA
  AGCCTGGTTCAATGAAAAGAGCAAGGAACTGACTACAGAAATTGATAATAACATTGAACA
  GATATCCAGCTATAAATCTGAGATTACTGAATTGAGACGTAATGTACAAGCTCTGGAGAT
  AGAACTACAGTCCCAACTGGCCTTGAAACAATCCCTGGAAGCCTCCTTGGCAGAAACAGA
  AGGTCGCTACTGTGTGCAGCTCTCACAGATTCAGGCCCAGATATCCGCTCTGGAAGAACA
  GTTGCAACAGATTCGAGCTGAAACCGAGTGCCAGAATACTGAATACCAACAACTCCTGGA
  TATTAAGATCCGACTGGAGAATGAAATTCAAACCTACCGCAGCCTGCTAGAAGGAGAGGG
  AAGTTCCGGAGGCGGCGGACGCGGCGGCGGAAGTTTCGGCGGCGGCTACGGCGGCGGAAG
  CTCCGGCGGCGGAAGCTCCGGCGGCGGCTACGGCGGCGGCCACGGCGGCAGTTCCGGCGG
  CGGCTACGGAGGCGGAAGCTCCGGCGGCGGAAGCTCCGGCGGCGGCTACGGGGGCGGAAG
  CTCCAGCGGCGGCCACGGCGGCAGTTCCAGCGGCGGCTACGGTGGTGGCAGTTCCGGCGG
  CGGCGGCGGCGGCTACGGGGGCGGCAGCTCCGGCGGCGGCAGCAGCTCCGGCGGCGGATA
  CGGCGGCGGCAGCTCCAGCGGAGGCCACAAGTCCTCCTCTTCCGGGTCCGTGGGCGAGTC
  TTCATCTAAGGGACCAAGATACTAACAAAACCAGAGTAATCAAGACAATTATTGAAGAGG
  TGGCGCCCGACGGTAGAGTTCTTTCATCTATGGTTGAATCAGAAACCAAGAAACACTACT
  ATTAAACTGCATCAAGAGGAGAGAGTCTCCCTTCACACAGACCATTATTTACAGATGCAT
  GGAAAACAAAGTCTCCAAGAAAACACTTCTGTCTTGATGGTCTATGGAAATAGACCTTGA
  AAATAAGGTGTCTACAAGGTGTTTTGTGGTTTCTGTATTTCTTCTTTTCACTTTACCAGA
  AAGTGTTCTTTAATGGAAAGAAAAACAACTTTCTGTTCTCATTTACTAATGAATTTCAAT
  AAACTTTCTTACTGATGCAAACTAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000421 unedited
  NGGGATTGGGGNNGAAAACCACNCTCTTAGGGCGGCCGCGATTCCCGGGATCAGCATCAC
  CATGTCTGTTCGATACAGCTCAAGCAAGCACTACTCTTCCTCCCGCAGTGGAGGAGGAGG
  AGGAGGAGGAGGATGTGGAGGAGGAGGAGGAGTGTCATCCCTAAGAATTTCTAGCAGCAA
  AGGCTCCCTTGGTGGAGGATTTAGCTCAGGGGGGTTCAGTGGTGGCTCTTTTAGCCGTGG
  GAGCTCTGGTGGGGGCTGCTTTGGGGGCTCATCAGGTGGCTATGGAGGATTAGGAGGTTT
  TGGTGGAGGTAGCTTTCGTGGAAGCTATGGAAGTAGCAGCTTTGGTGGGAGTTATGGAGG
  CAGCTTTGGAGGGGGCAGTTTCGGAGGTGGCAGCTTTGGTGGGGGCAGCTTTGGTGGAGG
  CGGCTTTGGTGGAGGCGGCTTTGGAGGAGGCTTTGGTGGTGGATTTGGAGGAGATGGTGG
  CCTTCTCTCTGGAAATGAAAAAGTAACCATGCAGAATCTGAATGACCGCCTGGCTTCCTA
  CTTGGACAAAGTTCGGGCTCTGGAAGAATCAAACTATGAGCTGGAAGGCAAAATCAAGGA
  GTGGTATGAAAAGCATGGCAACTCACATCAGGGGGAGCCTCGTGACTACAGCAAATACTA
  CAAAACCATCGATGACCTTAAAAATCAGATTCTCAACCTAACAACTGATAATGCCAACAT
  CCTGCTTCAGATCGACAATGCCAGGCTGGCAGCTGATGACTTCAGGCTGAAGTATGAGAA
  TGAGGTAGCTCTGCGCCAGAGCGTGGAGGCTGACATCAACGGCCTGCGTANGGTGCTGGA
  TGAGCTGACCCCTGACCAAGCTGACCTGNAGATGCANATTGAGAGCCTGACTGNNAGAGC
  TGCCCTATCTGAGAAGAACCACGAGA
  
• Restriction Sites: Please inquire
• ACCN: NM_000421
• Insert Size: 2145 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000421.2, NP_000412.2
• RefSeq Size: 2145 bp
• RefSeq ORF: 1755 bp
• Locus ID: 3858
• UniProt ID: P13645
• Cytogenetics: 17q21.2
• Gene Summary: This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]