Cytokeratin 10 (KRT10) (NM_000421) Human Untagged Clone

SKU SC122561

KRT10 (untagged)-Human keratin 10 (KRT10)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Cytokeratin 10
• Synonyms: BCIE; BIE; CK10; EHK; K10; KPP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000421 edited; CAGCATCACCATGTCTGTTCGATACAGCTCAAGCAAGCACTACTCTTCCTCCCGCAGTGG; AGGAGGAGGAGGAGGAGGAGGATGTGGAGGAGGAGGAGGAGTGTCATCCCTAAGAATTTC; TAGCAGCAAAGGCTCCCTTGGTGGAGGATTTAGCTCAGGGGGGTTCAGTGGTGGCTCTTT; TAGCCGTGGGAGCTCTGGTGGGGGCTGCTTTGGGGGCTCATCAGGTGGCTATGGAGGATT; AGGAGGTTTTGGTGGAGGTAGCTTTCGTGGAAGCTATGGAAGTAGCAGCTTTGGTGGGAG; TTATGGAGGCAGCTTTGGAGGGGGCAGTTTCGGAGGTGGCAGCTTTGGTGGGGGCAGCTT; TGGTGGAGGCGGCTTTGGTGGAGGCGGCTTTGGAGGAGGCTTTGGTGGTGGATTTGGAGG; AGATGGTGGCCTTCTCTCTGGAAATGAAAAAGTAACCATGCAGAATCTGAATGACCGCCT; GGCTTCCTACTTGGACAAAGTTCGGGCTCTGGAAGAATCAAACTATGAGCTGGAAGGCAA; AATCAAGGAGTGGTATGAAAAGCATGGCAACTCACATCAGGGGGAGCCTCGTGACTACAG; CAAATACTACAAAACCATCGATGACCTTAAAAATCAGATTCTCAACCTAACAACTGATAA; TGCCAACATCCTGCTTCAGATCGACAATGCCAGGCTGGCAGCTGATGACTTCAGGCTGAA; GTATGAGAATGAGGTAGCTCTGCGCCAGAGCGTGGAGGCTGACATCAACGGCCTGCGTAG; GGTGCTGGATGAGCTGACCCTGACCAAGGCTGACCTGGAGATGCAAATTGAGAGCCTGAC; TGAAGAGCTGGCCTATCTGAAGAAGAACCACGAGGAGGAAATGAAAGACCTTCGAAATGT; GTCCACTGGTGATGTGAATGTGGAAATGAATGCTGCCCCGGGTGTTGATCTGACTCAACT; TCTGAATAACATGAGAAGCCAATATGAACAACTTGCTGAACAAAACCGCAAAGATGCTGA; AGCCTGGTTCAATGAAAAGAGCAAGGAACTGACTACAGAAATTGATAATAACATTGAACA; GATATCCAGCTATAAATCTGAGATTACTGAATTGAGACGTAATGTACAAGCTCTGGAGAT; AGAACTACAGTCCCAACTGGCCTTGAAACAATCCCTGGAAGCCTCCTTGGCAGAAACAGA; AGGTCGCTACTGTGTGCAGCTCTCACAGATTCAGGCCCAGATATCCGCTCTGGAAGAACA; GTTGCAACAGATTCGAGCTGAAACCGAGTGCCAGAATACTGAATACCAACAACTCCTGGA; TATTAAGATCCGACTGGAGAATGAAATTCAAACCTACCGCAGCCTGCTAGAAGGAGAGGG; AAGTTCCGGAGGCGGCGGACGCGGCGGCGGAAGTTTCGGCGGCGGCTACGGCGGCGGAAG; CTCCGGCGGCGGAAGCTCCGGCGGCGGCTACGGCGGCGGCCACGGCGGCAGTTCCGGCGG; CGGCTACGGAGGCGGAAGCTCCGGCGGCGGAAGCTCCGGCGGCGGCTACGGGGGCGGAAG; CTCCAGCGGCGGCCACGGCGGCAGTTCCAGCGGCGGCTACGGTGGTGGCAGTTCCGGCGG; CGGCGGCGGCGGCTACGGGGGCGGCAGCTCCGGCGGCGGCAGCAGCTCCGGCGGCGGATA; CGGCGGCGGCAGCTCCAGCGGAGGCCACAAGTCCTCCTCTTCCGGGTCCGTGGGCGAGTC; TTCATCTAAGGGACCAAGATACTAACAAAACCAGAGTAATCAAGACAATTATTGAAGAGG; TGGCGCCCGACGGTAGAGTTCTTTCATCTATGGTTGAATCAGAAACCAAGAAACACTACT; ATTAAACTGCATCAAGAGGAGAGAGTCTCCCTTCACACAGACCATTATTTACAGATGCAT; GGAAAACAAAGTCTCCAAGAAAACACTTCTGTCTTGATGGTCTATGGAAATAGACCTTGA; AAATAAGGTGTCTACAAGGTGTTTTGTGGTTTCTGTATTTCTTCTTTTCACTTTACCAGA; AAGTGTTCTTTAATGGAAAGAAAAACAACTTTCTGTTCTCATTTACTAATGAATTTCAAT; AAACTTTCTTACTGATGCAAACTAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000421 unedited; NGGGATTGGGGNNGAAAACCACNCTCTTAGGGCGGCCGCGATTCCCGGGATCAGCATCAC; CATGTCTGTTCGATACAGCTCAAGCAAGCACTACTCTTCCTCCCGCAGTGGAGGAGGAGG; AGGAGGAGGAGGATGTGGAGGAGGAGGAGGAGTGTCATCCCTAAGAATTTCTAGCAGCAA; AGGCTCCCTTGGTGGAGGATTTAGCTCAGGGGGGTTCAGTGGTGGCTCTTTTAGCCGTGG; GAGCTCTGGTGGGGGCTGCTTTGGGGGCTCATCAGGTGGCTATGGAGGATTAGGAGGTTT; TGGTGGAGGTAGCTTTCGTGGAAGCTATGGAAGTAGCAGCTTTGGTGGGAGTTATGGAGG; CAGCTTTGGAGGGGGCAGTTTCGGAGGTGGCAGCTTTGGTGGGGGCAGCTTTGGTGGAGG; CGGCTTTGGTGGAGGCGGCTTTGGAGGAGGCTTTGGTGGTGGATTTGGAGGAGATGGTGG; CCTTCTCTCTGGAAATGAAAAAGTAACCATGCAGAATCTGAATGACCGCCTGGCTTCCTA; CTTGGACAAAGTTCGGGCTCTGGAAGAATCAAACTATGAGCTGGAAGGCAAAATCAAGGA; GTGGTATGAAAAGCATGGCAACTCACATCAGGGGGAGCCTCGTGACTACAGCAAATACTA; CAAAACCATCGATGACCTTAAAAATCAGATTCTCAACCTAACAACTGATAATGCCAACAT; CCTGCTTCAGATCGACAATGCCAGGCTGGCAGCTGATGACTTCAGGCTGAAGTATGAGAA; TGAGGTAGCTCTGCGCCAGAGCGTGGAGGCTGACATCAACGGCCTGCGTANGGTGCTGGA; TGAGCTGACCCCTGACCAAGCTGACCTGNAGATGCANATTGAGAGCCTGACTGNNAGAGC; TGCCCTATCTGAGAAGAACCACGAGA;
• Restriction Sites: Please inquire
• ACCN: NM_000421
• Insert Size: 2145 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000421.2, NP_000412.2
• RefSeq Size: 2145 bp
• RefSeq ORF: 1755 bp
• Locus ID: 3858
• UniProt ID: P13645
• Cytogenetics: 17q21.2
• Summary: This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]