CHRNA3 (NM_000743) Human Untagged Clone

CAT#: SC122160

CHRNA3 (untagged)-Human cholinergic receptor, nicotinic, alpha 3 (CHRNA3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHRNA3
• Synonyms: BAIPRCK; LNCR2; NACHRA3; PAOD2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC122160 sequence for NM_000743 edited (data generated by NextGen Sequencing)
  ATGGGCTCTGGCCCGCTCTCGCTGCCCCTGGCGCTGTCGCCGCCGCGGCTGCTGCTGCTG
  CTGCTGCTGTCTCTGCTGCCAGTGGCCAGGGCCTCAGAGGCTGAGCACCGTCTATTTGAG
  CGGCTGTTTGAAGATTACAATGAGATCATCCGGCCTGTAGCCAACGTGTCTGACCCAGTC
  ATCATCCATTTCGAGGTGTCCATGTCTCAGCTGGTGAAGGTGGATGAAGTAAACCAGATC
  ATGGAGACCAACCTGTGGCTCAAGCAAATCTGGAATGACTACAAGCTGAAGTGGAACCCC
  TCTGACTATGGTGGGGCAGAGTTCATGCGTGTCCCTGCACAGAAGATCTGGAAGCCAGAC
  ATTGTGCTGTATAACAATGCTGTTGGGGATTTCCAGGTGGACGACAAGACCAAAGCCTTA
  CTCAAGTACACTGGGGAGGTGACTTGGATACCTCCGGCCATCTTTAAGAGCTCCTGTAAA
  ATCGACGTGACCTACTTCCCGTTTGATTACCAAAACTGTACCATGAAGTTCGGTTCCTGG
  TCCTACGATAAGGCGAAAATCGATCTGGTCCTGATCGGCTCTTCCATGAACCTCAAGGAC
  TATTGGGAGAGCGGCGAGTGGGCCATCATCAAAGCCCCAGGCTACAAACACGACATCAAG
  TACAACTGCTGCGAGGAGATCTACCCCGACATCACATACTCGCTGTACATCCGGCGCCTG
  CCCTTGTTCTACACCATCAACCTCATCATCCCCTGCCTGCTCATCTCCTTCCTCACTGTG
  CTCGTCTTCTACCTGCCCTCCGACTGCGGTGAGAAGGTGACCCTGTGCATTTCTGTCCTC
  CTCTCCCTGACGGTGTTTCTCCTGGTGATCACTGAGACCATCCCTTCCACCTCGCTGGTC
  ATCCCCCTGATTGGAGAGTACCTCCTGTTCACCATGATTTTTGTAACCTTGTCCATCGTC
  ATCACCGTCTTCGTGCTCAACGTGCACTACAGAACCCCGACGACACACACAATGCCCTCA
  TGGGTGAAGACTGTATTCTTGAACCTGCTCCCCAGGGTCATGTTCATGACCAGGCCAACA
  AGCAACGAGGGCAACGCTCAGAAGCCGAGGCCCCTCTACGGTGCCGAGCTCTCAAATCTG
  AATTGCTTCAGCCGCGCAGAGTCCAAAGGCTGCAAGGAGGGCTACCCCTGCCAGGACGGG
  ATGTGTGGTTACTGCCACCACCGCAGGATAAAAATCTCCAATTTCAGTGCTAACCTCACG
  AGAAGCTCTAGTTCTGAATCTGTTGATGCTGTGCTGTCCCTCTCTGCTTTGTCACCAGAA
  ATCAAAGAAGCCATCCAAAGTGTCAAGTATATTGCTGAAAATATGAAAGCACAAAATGAA
  GCCAAAGAGATTCAAGATGATTGGAAGTATGTTGCCATGGTGATTGATCGTATTTTTCTG
  TGGGTTTTCACCCTGGTGTGCATTCTAGGGACAGCAGGATTGTTTCTGCAACCCCTGATG
  GCCAGGGAAGATGCATAA
  
  Clone variation with respect to NM_000743.4
  291 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000743 unedited
  ATCAAATATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGTCCTCC
  CGCGGNGTCCGAGGGGCGCTGGNAAACCCAGCGGCGGCGAAGCGGAGAGGAGCCCCGCGC
  GTCTCCGCCCGCACGGCTCCAGGTCTGGGGTCTGCGCTGGAGCCGCGCGGGGAGAGGCCG
  TCTCTGCGACCGCCGCGCCCGCTCCCGACCGTCCGGGTCCGCGGCCAGCCCGGCCACCAG
  CCATGGGCTCTGGCCCGCTCTCGCTGCCCCTGGCGCTGTCGCCGCCGCGGCTGCTGCTGC
  TGCTGCTGCTGTCTCTGCTGCCAGTGGCCAGGGCCTCAGAGGCTGAGCACCGTCTATTTG
  AGCGGCTGTTTGAAGATTACAATGAGATCATCCGGCCTGTAGCCAACGTGTCTGACCCAG
  TCATCATCCATTTCGAGGTGTCCATGTCTCAGCTGGTGAAGGTGGATGAAGTAAACCAGA
  TCATGGAGACCAACCTGTGGCTCAAGCAAATCTGGAATGACTACAAGCTGAAGTGGAACC
  CCTCTGACTATGGTGGGGCAGAGTTCATGCGTGTCCCTGCACAGAAGATCTGGAAGCCAG
  ACATTGTGCTGTATAACAATGCTGTTGGGGATTTCCAGGTGGACGANCAGACCAAAGCCT
  TTACTCAGTACACTGGGGGAGGTGACTTGGGATACCTNCGGCCCATCTTNAGAGCTCCTG
  GTAAATCGACGTGACCTACTTCCCGTTTGATTACCCAAAACTGTGCCCTGAAATTCGGGG
  TCCTGGTGCCTACAATAAGGCAAAAATCGATCCTGGTCCTGATCCGGCTCTTCCATGACC
  CTCCAGAACCTATGGGAGAAGCGGCGAATGGGCCCATTATAAAACCCCCGGGCTACAAAC
  ACCAAATTAGGTCCAACTGGTTGGAAGGGAATCTTCCCGCT
  
• Restriction Sites: Please inquire
• ACCN: NM_000743
• Insert Size: 1518 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000743.2, NP_000734.2
• RefSeq Size: 3020 bp
• RefSeq ORF: 1518 bp
• Locus ID: 1136
• UniProt ID: P32297
• Cytogenetics: 15q25.1
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Gene Summary: This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).