splicing factor 1 (SF1) (NM_201997) Human Untagged Clone

CAT#: SC121559

SF1 (untagged)-Human splicing factor 1 (SF1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: splicing factor 1
• Synonyms: BBP; D11S636; MBBP; ZCCHC25; ZFM1; ZNF162
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC121559 sequence for NM_201997 edited (data generated by NextGen Sequencing)
  ATGGCGACCGGAGCGAACGCCACGCCGTTGGACTTCCCAAGTAAGAAGCGGAAGAGGAGC
  CGCTGGAACCAAGACACAATGGAACAGAAGACAGTGATTCCAGGAATGCCTACAGTTATT
  CCCCCTGGACTTACTCGAGAACAAGAAAGAGCTTATATAGTGCAACTGCAGATAGAAGAC
  CTGACTCGTAAACTGCGCACAGGAGACCTGGGCATCCCCCCTAACCCTGAGGACAGGTCC
  CCTTCCCCTGAGCCCATCTACAATAGCGAGGGGAAGCGGCTTAACACCCGAGAGTTCCGC
  ACCCGCAAAAAGCTGGAAGAGGAGCGGCACAACCTCATCACAGAGATGGTTGCACTCAAT
  CCGGATTTCAAGCCACCTGCAGATTACAAACCTCCAGCAACACGTGTGAGTGATAAAGTC
  ATGATTCCACAAGATGAGTACCCAGAAATCAACTTTGTGGGGCTGCTCATCGGGCCCAGA
  GGGAACACCCTGAAGAACATAGAGAAGGAGTGCAATGCCAAGATTATGATCCGGGGGAAA
  GGGTCTGTGAAAGAAGGGAAGGTTGGGCGCAAAGATGGCCAGATGTTGCCAGGAGAAGAT
  GAGCCACTTCATGCCCTGGTTACTGCCAATACAATGGAGAACGTCAAAAAGGCAGTGGAA
  CAGATAAGAAACATCCTGAAGCAGGGTATCGAGACTCCAGAGGACCAGAATGATCTACGG
  AAGATGCAGCTTCGGGAGTTGGCTCGCTTAAATGGGACCCTTCGGGAAGACGATAACAGG
  ATCTTAAGACCCTGGCAGAGCTCAGAGACCCGCAGCATTACCAACACCACAGTGTGTACC
  AAGTGTGGAGGGGCTGGCCACATTGCTTCAGACTGTAAATTCCAAAGGCCTGGTGATCCT
  CAGTCAGCTCAGGATAAAGCACGGATGGATAAAGAATATTTGTCCCTCATGGCTGAACTG
  GGTGAAGCACCTGTCCCAGCATCTGTGGGCTCCACCTCTGGGCCTGCCACCACACCCCTG
  GCCAGCGCACCTCGTCCTGCTGCTCCCGCCAACAACCCACCTCCACCGTCTCTCATGTCT
  ACCACCCAGAGCCGCCCACCCTGGATGAATTCTGGCCCTTCAGAGAGTCGGCCCTACCAC
  GGCATGCATGGAGGTGGTCCTGGTGGGCCCGGAGGTGGCCCCCACAGCTTCCCACACCCA
  TTACCCAGCCTGACAGGTGGGCATGGTGGACATCCCATGCAGCACAACCCCAATGGACCC
  CCACCCCCTTGGATGCAGCCACCACCACCACCGATGAACCAGGGCCCCCACCCTCCTGGG
  CACCATGGCCCTCCTCCAANNNNNNNNTCAGTACCTGGGAAGTACGCCTGTGGGCTCTGG
  GGTCTATCGCCTGCATCAAGGAAAAGGTATGATGCCGCCACCACCTATGGGCATGATGCC
  GCCGCCGCCGCCGCCTCCCAGTGGGCAGCCCCCACCCCCTCCCTCTGGTCCTCTTCCCCC
  ATGGCAACAACAGCAGCAGCAGCCTCCGCCACCCCCTCCGCCCAGCAGCAGTATGGCTTC
  CAGTACCCCCTTGCCATGGCAGCAAAGATCCCTCCCCGCGGCGGCGATGGCCCGAGCCAT
  GAGAGTGAGGACTTTCCGCGCCCATTGGTGACCCTTCCAGGCAGACAGCCTCAGCAACGC
  CCCTGGTGGACAGGATGGTTCGGCAAAGCAGCCTGA
  
  Clone variation with respect to NM_201997.2
  1340 t=>n;1341 g=>n;1342 g=>n;1343 g=>n;1344 t=>n;1345 a=>n;1346 a=>n;1347 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_201997 unedited
  AATATCCCCCGCCCGTTGGCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
  GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGGCCGCGTGAGAGGACGGGCTCCGCGCGCTCCGGCAGCGCATTCG
  GGTCCCCTCCCCCCGGGAGGCTTGCGAAGGAGAAGCCGCCGCAGAGGAAAAGCAGGTGCC
  GGTGCCTGTCCCCGGGGGCGCCATGGCGACCGGAGCGAACGCCACGCCGTTGGACTTCCC
  AAGTAAGAAGCGGAAGAGGAGCCGCTGGAACCAAGACACAATGGAACAGAAGACAGTGAT
  TCCAGGAATGCCTACAGTTATTCCCCCTGGACTTACTCGAGAACAAGAAAGAGCTTATAT
  AGGTAAATATACGTTCTGCATCCAAATGCACTTTGAGAGTGTTACGAGTTATTCTGAGCA
  AGAGCTGCATTTTTTGAAAGTTTGAGAGTGTAGTTATCTTTTTTGAGATGAGAGACTTTC
  ACGACTTTGGTATCATCTGTCAGTTTTTGCCCAGTGAGTTCTGCATTTGCAGCTCCTTGT
  TTTGGTCTGTTCGTAACTGCAGTGTTCTCCATGAGTATCAGGAAGGTAGGGTTTCACTTA
  GGAGTAAGAACAGTCCTCAGTCCAGCAGCCACCCTTTTCAGCTGCTGTTCATTGCCAGTT
  GATGAGGTGAGTGTCATCTGCCTCTCTGGACAGGCCCCAGTGGAGAGCACTGCTAGTACT
  GNTCACCAAGTACTTTTCACAGCGTGGCCTTTCACCATCATTNTATATTTCTCCCCCTAG
  AAGGNGGNCTTTTACTTTTATAGGTACTTTTCTGAAGACAAAATGACACAAGTGGTTCTG
  AAGTGGTGTTACAGTTACCTCTTTTATTTCTTGAAGTTAGAGTTAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_201997
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201997.1, NP_973726.1
• RefSeq Size: 1001 bp
• RefSeq ORF: 243 bp
• Locus ID: 7536
• Cytogenetics: 11q13.1
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]
  Transcript Variant: This variant (4) has multiple coding region differences, compared to variant 1, one of which results in a frameshift. It encodes isoform 4, which is shorter and has a distinct C-terminus, compared to isoform 1.