TMEPAI (PMEPA1) (NM_199170) Human Untagged Clone

CAT#: SC121025

PMEPA1 (untagged)-Human prostate transmembrane protein, androgen induced 1 (PMEPA1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TMEPAI
• Synonyms: STAG1; TMEPAI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC121025 sequence for NM_199170 edited (data generated by NextGen Sequencing)
  ATGATGGTGATGGTGGTGGTGATCACGTGCCTGCTGAGCCACTACAAGCTGTCTGCACGG
  TCCTTCATCAGCCGGCACAGCCAGGGGCGGAGGAGAGAAGATGCCCTGTCCTCAGAAGGA
  TGCCTGTGGCCCTCGGAGAGCACAGTGTCAGGCAACGGAATCCCAGAGCCGCAGGTCTAC
  GCCCCGCCTCGGCCCACCGACCGCCTGGCCGTGCCGCCCTTCGCCCAGCGGGAGCGCTTC
  CACCGCTTCCAGCCCACCTATCCGTACCTGCAGCACGAGATCGACCTGCCGCCCACCATC
  TCGCTGTCAGACGGGGAGGAGCCCCCACCCTACCAGGGCCCCTGCACCCTCCAGCTTCGG
  GACCCCGAGCAGCAGCTGGAACTGAACCGGGAGTCGGTGCGCGCACCCCCAAACAGAACC
  ATCTTCGACAGTGACCTGATGGATAGTGCCAGGCTGGGCGGCCCCTGCCCCCCCAGCAGT
  AACTCGGGCATCAGCGCCACGTGCTACGGCAGCGGCGGGCGCATGGAGGGGCCGCCGCCC
  ACCTACAGCGAGGTCATCGGCCACTACCCGGGGTCCTCCTTCCAGCACCAGCAGAGCAGT
  GGGCCGCCCTCCTTGCTGGAGGGGACCCGGCTCCACCACACACACATCGCGCCCCTAGAG
  AGCGCAGCCATCTGGAGCAAAGAGAAGGATAAACAGAAAGGACACCCTCTCTAG
  
  Clone variation with respect to NM_199170.1
  291 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_199170 unedited
  GTATACACTACTATAGGGCGGCCGCGAATTCGCACGAGGATCGTGGTGGTGATGATGGTG
  ATGGTGGTGGTGATCACGTGCCTGCTGAGCCACTACAAGCTGTCTGCACGGTCCTTCATC
  AGCCGGCACAGCCAGGGGCGGAGGAGAGAAGATGCCCTGTCCTCAGAAGGATGCCTGTGG
  CCCTCGGAGAGCACAGTGTCAGGCAACGGAATCCCAGAGCCGCAGGTCTACGCCCCGCCT
  CGGCCCACCGACCGCCTGGCCGTGCCGCCCTTCGCCCAGCGGGAGCGCTTCCACCGCTTC
  CAGCCCACCTATCCGTACCTGCAGCACGAGATCGACCTGCCGCCCACCATCTCGCTGTCA
  GACGGGGAGGAGCCCCCACCCTACCAGGGCCCCTGCACCCTCCAGCTTCGGGACCCCGAG
  CAGCAGCTGGAACTGAACCGGGAGTCGGTGCGCGCACCCCCAAACAGAACCATCTTCGAC
  AGTGACCTGATGGATAGTGCCAGGCTGGGCGGCCCCTGCCCCCCCAGCAGTAACTCGGGC
  ATCAGCGCCACGTGCTACGGCAGCGGCGGGCGCATGGAGGGGCCGCCGCCCACCTACAGC
  GAGGTCATCGGCCACTACCCGGNGTCCTCCTTCCAGCACCAGCAGAGCAGTGGGCCGCCC
  TCCTTGCTGGAGGGGACCCGGCTCCACCACACACACATCGCGCCCCTAGAGAGCGCAGCC
  ATCTGGAGCANAGAGAAAGATAAACAGAAAGGACACCCTCTCTAGGGTCCCCAGGGGGGC
  CGGGCTGGNGCTGCCGTAGTGAAAGGCAAACACTNCGCGCTTCTAAAAAAGATGANNAGA
  AGCGGGGGGCGCACCACGCATCGTGTGCCCCTCCCTCCACCTCCTGTGATAATTTTACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_199170
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199170.1, NP_954639.1
• RefSeq Size: 4531 bp
• RefSeq ORF: 714 bp
• Locus ID: 56937
• UniProt ID: Q969W9
• Cytogenetics: 20q13.31
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a transmembrane protein that contains a Smad interacting motif (SIM). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins. Overexpression of this gene may play a role in multiple types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
  Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. Variants 3 and 4 encode the same isoform (c), which has a shorter N-terminus compared to isoform a.