Citrate transport protein (SLC25A1) (NM_005984) Human Untagged Clone

CAT#: SC120727

SLC25A1 (untagged)-Human solute carrier family 25 (mitochondrial carrier, citrate transporter), member 1 (SLC25A1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Citrate transport protein
• Synonyms: CMS23; CTP; D2L2AD; SEA; SLC20A3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120727 sequence for NM_005984 edited (data generated by NextGen Sequencing)
  ATGCCCGCGCCCCGCGCCCCGCGCGCTCTGGCGGCCGCCGCGCCCGCGTCCGGGAAGGCC
  AAGCTGACGCACCCGGGGAAGGCGATCCTGGCAGGCGGCCTGGCGGGTGGCATCGAGATC
  TGCATCACCTTCCCCACCGAGTACGTGAAGACGCAGCTGCAGCTGGACGAGCGCTCGCAC
  CCGCCGCGGTACCGGGGCATCGGGGACTGCGTGCGGCAGACGGTTCGCAGCCATGGCGTC
  CTGGGCCTGTACCGCGGCCTTAGCTCCCTGCTCTACGGTTCCATCCCCAAGGCGGCCGTC
  AGGTTTGGAATGTTCGAGTTCCTCAGCAACCACATGCGGGATGCCCAGGGACGGCTGGAC
  AGCACGCGTGGGCTGCTGTGCGGCCTGGGCGCTGGCGTGGCCGAGGCCGTGGTGGTCGTG
  TGCCCCATGGAGACCATCAAGGTGAAGTTCATCCACGACCAGACCTCCCCAAACCCCAAG
  TACAGAGGATTCTTCCACGGGGTTAGGGAGATTGTGCGGGAACAAGGGCTGAAGGGGACG
  TACCAGGGCCTCACAGCCACTGTCCTGAAGCAGGGCTCGAACCAGGCCATCCGCTTCTTC
  GTCATGACCTCCCTGCGCAACTGGTACCGAGGGGACAACCCCAACAAGCCCATGAACCCT
  CTGATCACTGGGGTCTTCGGAGCTATTGCAGGCGCAGCCAGTGTCTTTGGAAACACTCCT
  CTGGATGTGATTAAGACCCGGATGCAGGGCCTGGAGGCGCACAAATACCGGAACACGTGG
  GACTGCGGCTTGCAGATCCTGAAGAAGGAGGGGCTCAAGGCATTCTACAAGGGCACTGTC
  CCCCGCCTGGGCCGGGTCTGCCTGGATGTGGCCATAGTGTTTGTCATCTATGATGAAGTG
  GTGAAGCTGCTCAACAAAGTGTGGAAGACGGACTAA
  
  Clone variation with respect to NM_005984.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005984 unedited
  NGGTGCACATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCTGG
  AGTCTCGGACCCGAACCGCCACAGGCGCCCCGCCTCCCGCCCGCCATGCCCGCGCCCCGC
  GCCCCGCGCGCTCTGGCGGCCGCCGCGCCCGCGTCCGGGAAGGCCAAGCTGACGCACCCG
  GGGAAGGCGATCCTGGCAGGCGGCCTGGCGGGTGGCATCGAGATCTGCATCACCTTCCCC
  ACCGAGTACGTGAAGACGCAGCTGCAGCTGGACGAGCGCTCGCACCCGCCGCGGTACCGG
  GGCATCGGGGACTGCGTGCGGCAGACGGTTCGCAGCCATGGCGTCCTGGGCCTGTACCGC
  GGCCTTAGCTCCCTGCTCTACGGTTCCATCCCCAAGGCGGCCGTCAGGTTTGGAATGTTC
  GAGTTCCTCAGCAACCACATGCGGGATGCCCAGGGACGGCTGGACAGCACGCGTGGGCTG
  CTGTGCGGCCTGGGCGCTGGCGTGGCCGAGGCCGTGGTGGTCGTGTGCCCCATGGAGACC
  ATCAAGGTGAAGTTCATCCACGACCAGACCTCCCCAAACCCCAAGTACAGAGGATTCTTC
  CACGGGGTTAGGGAGATTGTGCGGGAACAAGGGCTGAAGGGGACGTACCAGGGCCTCACA
  GCCACTGTCCTGAAGCAGGGCTCGAACCAGGCCATCCGCTTCTTCGTCATGACCTCCCTG
  CGCAACTGGTACCGAGGGGACAACCCCAACAAGCCCATGAACCCTCTGATCACTGGGGTC
  TTCGGAGCTATTGCAGGCGCAGCCAGTGTCTTTGGAACACTCCTCTGGATGTGATTAAGA
  CCCGNATGCANGGCCCTGGAGCGCANCAATACCGGNACACGTGGGAACTGCGCTTGCAGA
  TCCTGAAGAAGGAGGGCTCAAGCATTCTACAGGGCACTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005984 unedited
  NTTTTCGCTCTGCACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGCA
  ACAGGATCCGGTTTATTCTGCCTTGGCAGGGTGGTCCTGAGAGTGGTGGGTGCCACCCTG
  TCCGGGGCGGAGAGAGGGCCCGAGGGCCAGTTAAGGCCAATGGCGGGAGAAGCAGGGGGC
  TGCAGCCCCTGGAATGCGGTGAAGCCAGGCCGAGGCCCGGAGGCAGCTGTGGTAGGCCAG
  GGCAGGGTGGAAGGCACCGGACTGGGACCGGGCCAGGGCTACAGGGCCGAGGACCCAGGC
  CACACGGGCACCCCGGGAGGCGGGGCACAGGGTCACGTGACACAGAACATGAAACACAGG
  CACAGGGTCATAGGCCAGATGCACATCCAGCCATGGCTGGGCCAGACACTGGGACACAGT
  GGTGGTGTCACACACAGACCACAGGGGGGACACATGGATTTGACAGCCACAATGCACAGA
  CCAGGCTACAGAGCTCGAGGGACGTGGGAAGGGGCCTTCTGGCACTACTGCACTGGAATC
  GTGAGACAAAGGTAGCAGGACACTCTGGCGGTGCCTGGGGCGGCCCCCTTGCGGGCTCTC
  TGGCTTAGTCCGTCCTCCACACTTTGTTGAGCAGCTTCACCACTTCATCATAGATGACAA
  ACACTATTGCCCACATCCACGCCAGACCCGGGCCCCAGGCCGGGGGACAGTGCCCCTTGT
  AAAATCCTTTGACCCCCTCCTTTTTTAGGGATCTGAAGCCGCAGTCCCACGTGGTCCGTG
  TTTTGTGCGCCTCCAGCCCCTGCATTCGGGTCCTAATCACATCCAGAAGAATGTTCCAAA
  AACCTGCCTGGCCTGGAAAACTCCCAAAACCCAGGACCAAAGGTTCATGCCTTTGTGGGT
  TGCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005984
• Insert Size: 1460 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005984.1, NP_005975.1
• RefSeq Size: 1619 bp
• RefSeq ORF: 936 bp
• Locus ID: 6576
• UniProt ID: P53007
• Cytogenetics: 22q11.21
• Domains: mito_carr
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the mitochondrial carrier subfamily of solute carrier proteins. Members of this family include nuclear-encoded transporters that translocate small metabolites across the mitochondrial membrane. This protein regulates the movement of citrate across the inner membranes of the mitochondria. Mutations in this gene have been associated with combined D-2- and L-2-hydroxyglutaric aciduria. Pseudogenes of this gene have been identified on chromosomes 7, 11, 16, and 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
  Transcript Variant: This variant (1) encodes the shorter isoform (a, also known as mCiC).