ALKBH3 (NM_139178) Human Untagged Clone

CAT#: SC120570

ALKBH3 (untagged)-Human alkB, alkylation repair homolog 3 (E. coli) (ALKBH3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALKBH3
• Synonyms: ABH3; DEPC-1; DEPC1; hABH3; PCA1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_139178 edited
  ATGGAGGAAAAAAGACGGCGAGCCCGAGTTCAGGGAGCCTGGGCTGCCCCTGTTAAAAGC
  CAGGCCATTGCTCAGCCAGCTACCACTGCTAAGAGCCATCTCCACCAGAAGCCTGGCCAG
  ACCTGGAAGAACAAAGAGCATCATCTCTCTGACAGAGAGTTTGTGTTCAAAGAACCTCAG
  CAGGTAGTACGTAGAGCTCCTGAGCCACGAGTGATTGACAGAGAGGGTGTGTATGAAATC
  AGCCTGTCACCCACAGGTGTATCTAGGGTCTGTTTGTATCCTGGCTTTGTTGACGTGAAA
  GAAGCTGACTGGATATTGGAACAGCTTTGTCAAGATGTTCCCTGGAAACAGAGGACCGGC
  ATCAAAGAGGATATAACTTATCAGCAACCAAGACTTACAGCATGGTATGGAGAACTTCCT
  TACACTTATTCAAGAATCACTATGGAACCAAATCCTCACTGGCACCCTGTGCTGCGCACA
  CTAAAGAACCGCATTGAAGAGAACACTGGCCACACCTTCAACTCCTTACTCTGCAATCTT
  TATCGCAATGAGAAGGACAGCGTGGACTGGCACAGTGATGATGAACCCTCACTAGGGAGG
  TGCCCCATTATTGCTTCACTAAGTTTTGGTGCCACACGCACATTTGAGATGAGAAAGAAG
  CCACCACCAGAAGAGAATGGAGAGTACACATATGTGGAAAGAGTGAAGATACCCTTGGAT
  CATGGGACCTTGTTAATCATGGAAGGAGCGACACAAGCTGACTGGCAGCATCGAGTGCCC
  AAAGAATACCACTCTAGAGAACCGAGAGTGAACCTGACCTTTCGGACAGTCTATCCAGAC
  CCTCGAGGGGCACCCTGGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_139178 unedited
  GGGGTTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGCGCGCC
  CGGCTCCGCCCGCAAGTGCGCCTTCCTGACTTACTGCTGGGTGCGCGGGGCTGGGGGTGC
  GAGTACCACCCCTGAAGTCTCTTCCTGGGCGACCTCCGGGGCCTCATTCTAGGCCTCCTT
  AAAGAGAAGGATCTAAATTAGGAAAAGGAAGTGCCCTTATCCACGACCAAGCTCTTCCAC
  CTGCGGAGCTCGCTTAGTCTGCACCTCAACCGTGCGGAAAGTGACTGCCCTGTTTACTGA
  GGAAAAACTGGGGCTCAGAAAGATACCATGGAGTAGTTTGAAACAGGAACAAAATCTTCT
  GAAAGCTCGGAGCAGAAGCCTTTTTGGTCAACATGGAGGAAAAAAGACGGCGAGCCCGAG
  TTCAGGGAGCCTGGGCTGCCCCTGTTAAAAGCCAGGCCATTGCTCAGCCAGCTACCACTG
  CTAAGAGCCATCTCCACCAGAAGCCTGGCCAGACCTGGAAGAACAAAGAGCATCATCTCT
  CTGACAGAGAGTTTGTGTTCAAAGAACCTCAGCAGGTAGTACGTAGAGCTCCTGAGCCAC
  GAGTGATTGACAGAGAGGGTGTGTATGAAATCAGCCTGTCACCCACAGGTGTATCTAGGG
  TCTGTTTGTATCCTGGCTTTGTTGACGTGAAAGAAGCTGACTGGATATTGGAACAGCTTT
  GTCAAGATGTTCCCTGGAAACAGAGGACCGGCATCAAAGAGGATATAACTTATCAGCAAC
  CAAGACTTACAGCATGGTATGGAGAACTTCCTTACACTTATTCAAGAATCACTATGGAAC
  CAAATNCTCACTGGCACCCTGTGCTGCGCACACTAAAGAACCGCATTTGAGAGAACACTG
  GCCACACTTNCACTCCTTACTCTGCAATCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_139178 unedited
  NNGGCCTTGACCTGCCCATANATTTAATTGCCTAAGNAGATAGCCATTTTGAGCTGTGGT
  TAGGGATAACTGTTCCCACAGTAGACCTATTACCAACATGAGTTGCTGGCTTTCATTTAA
  TAGGACTCCAAGCTGGCAAACAAACCCCACCATCTTCCCAACAGCCACATCATGAGATCT
  AGCAGCACCAGCCTCTTGAAGTGGCTTCTCAGTGGAAGGTTTGCTCCGTTTCAGTGAAGC
  TTCTCTCTCAAAGCTCTGACGTCACCAGGGTGCCCCTCGAGGGTCTGGATAGACTGTCCG
  AAAGGTCAGGTTCACTCTCGGTTCTCTAGAGTGGTATTCTTTGGGCACTCGATGCTGCCA
  GTCAGCTTGTGTCGCTCCTTCCATGATTAACAAGGTCCCATGATCCAAGGGTATCTTCAC
  TCTTTCCACATATGTGTACTCTCCATTCTCTTCTGGTGGTGGCTTCTTTCTCATCTCAAA
  TGTGCGTGTGGCACCAAAACTTAGTGAAGCAATAATGGGGCACCTCCCTAGTGAGGGTTC
  ATCATCACTGTGCCAGTCCACGCTGTCCTTCTCATTGCGATAAAGATTGCAGAGTAAGGA
  GTTGAAGGTGTGGCCAGTGTTCTCTTCAATGCGGTTCTTTAGTGTGCGCAGCACAGGGTG
  CCAGTGAGGATTTGGTTCCATAGTGATTCTTGAATAAGTGTAAGGAAGTTCTCCATACCA
  TGCTGTAAGTCTTGGTTGCTGATAAGTTATATCCTCTTTGATGCCGGTCCTCTGTTTCCA
  GGGAACATCTTGACAAAGCTGTTCCCATATCCAGTCAGCTTCTTTCACGTCAACAAAGCC
  AGGATACAAACAGACCCTAGATACACCTGTGGGGTGACAGGCTGATTTCATAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_139178
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139178.1, NP_631917.1
• RefSeq Size: 1520 bp
• RefSeq ORF: 861 bp
• Locus ID: 221120
• UniProt ID: Q96Q83
• Cytogenetics: 11p11.2
• Domains: 2OG-FeII_Oxy
• Protein Families: Druggable Genome
• Gene Summary: The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008]