DAAM1 (NM_014992) Human Untagged Clone

CAT#: SC120568

DAAM1 (untagged)-Human dishevelled associated activator of morphogenesis 1 (DAAM1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DAAM1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120568 sequence for NM_014992 edited (data generated by NextGen Sequencing)
  ATGGCCCCAAGAAAGAGAGGTGGACGAGGTATTTCATTCATCTTTTGCTGTTTCCGAAAT
  AATGATCACCCAGAAATCACGTATCGGCTGCGAAATGATAGCAACTTTGCGCTTCAGACC
  ATGGAACCAGCATTGCCCATGCCCCCTGTGGAGGAGCTGGATGTCATGTTCAGTGAACTG
  GTGGATGAACTGGACCTCACAGACAAACACAGAGAAGCCATGTTTGCACTTCCAGCTGAG
  AAAAAATGGCAAATATACTGTAGCAAGAAAAAGGACCAGGAAGAAAACAAGGGAGCTACA
  AGTTGGCCTGAATTCTACATTGATCAGCTCAATTCCATGGCTGCTAGAAAATCTCTGCTG
  GCTTTAGAGAAGGAAGAAGAAGAAGAAAGAAGTAAAACTATAGAGAGTTTAAAGACAGCA
  CTGAGGACAAAACCAATGAGGTTTGTAACCAGATTCATCGACTTGGATGGCCTATCATGT
  ATCCTCAACTTTCTAAAGACCATGGACTACGAGACCTCAGAGTCTCGAATACATACTTCT
  CTCATTGGCTGTATAAAGGCGTTAATGAACAACTCTCAAGGCCGGGCTCACGTCCTGGCT
  CATTCTGAGAGTATTAATGTAATTGCTCAGAGTCTGAGCACAGAGAACATTAAAACGAAG
  GTGGCCGTGCTGGAAATCTTGGGCGCCGTGTGCCTGGTTCCCGGGGGCCACAAGAAGGTT
  CTGCAGGCCATGCTGCACTACCAGAAGTATGCCAGCGAAAGGACCCGCTTTCAGACATTA
  ATTAACGACTTGGATAAAAGCACTGGGCGGTATCGAGATGAAGTGAGTCTCAAGACTGCC
  ATCATGTCCTTCATTAATGCAGTGCTCAGCCAAGGTGCAGGAGTGGAGAGTTTGGACTTT
  AGACTTCATCTTCGCTATGAATTTCTGATGTTAGGAATTCAACCTGTAATAGATAAATTA
  AGGGAACACGAAAATTCAACATTAGATAGGCATTTAGACTTTTTTGAAATGCTCCGAAAT
  GAAGATGAACTAGAATTTGCCAAAAGATTTGAACTGGTTCACATAGACACAAAAAGTGCA
  ACTCAGATGTTTGAGCTGACCAGGAAGAGGCTGACACATAGTGAAGCTTACCCGCATTTC
  ATGTCCATCCTGCACCACTGCCTCCAAATGCCTTACAAGAGGAGTGGCAACACTGTTCAG
  TACTGGCTACTACTAGATAGAATTATACAGCAGATAGTTATCCAGAATGACAAAGGACAG
  GACCCTGACTCCACACCTTTGGAAAACTTTAATATTAAGAATGTCGTACGAATGTTGGTT
  AATGAAAATGAAGTTAAGCAGTGGAAAGAACAAGCGGAAAAAATGAGAAAAGAGCACAAT
  GAGCTACAGCAGAAACTGGAAAAGAAAGAACGAGAATGTGATGCTAAGACTCAAGAGAAG
  GAAGAGATGATGCAGACCTTAAATAAAATGAAAGAGAAACTTGAAAAGGAGACTACTGAG
  CATAAGCAAGTCAAGCAGCAGGTGGCGGACCTCACAGCACAGCTCCATGAGCTCAGCAGG
  AGGGCCGTCTGTGCTTCAATCCCAGGTGGACCCTCGCCTGGAGCACCAGGAGGGCCCTTT
  CCTTCCTCTGTGCCTGGATCTCTCCTTCCTCCCCCACCACCCCCACCTCTACCAGGTGGG
  ATGCTTCCCCCTCCACCGCCTCCCCTCCCTCCAGGTGGCCCTCCTCCTCCCCCAGGGCCT
  CCTCCCTTAGGGGCAATCATGCCACCTCCTGGTGCTCCAATGGGCCTAGCACTGAAGAAG
  AAAAGCATTCCTCAGCCCACAAATGCCCTGAAATCCTTCAACTGGTCTAAACTGCCCGAG
  AACAAACTGGAAGGAACAGTATGGACCGAAATTGATGATACAAAAGTCTTCAAAATTCTA
  GATCTTGAAGACCTGGAAAGAACCTTCTCTGCCTATCAAAGACAGCAGGATTTCTTTGTG
  AACAGTAACTCCAAGCAGAAAGAAGCAGATGCCATTGATGACACTCTGAGTTCCAAACTT
  AAAGTTAAAGAGCTTTCGGTGATTGATGGTCGGAGAGCTCAGAATTGCAACATCCTTCTA
  TCGAGGTTGAAATTATCCAATGACGAAATCAAACGGGCAATTCTAACAATGGACGAACAG
  GAAGATCTGCCCAAGGACATGTTGGAACAGCTCTTGAAATTTGTTCCTGAAAAAAGTGAC
  ATTGACCTATTGGAGGAACATAAACACGAACTGGATCGGATGGCCAAGGCTGATAGGTTC
  CTTTTTGAGATGAGCCGAATTAATCACTATCAGCAAAGGTTGCAATCGCTGTACTTCAAA
  AAGAAGTTTGCAGAGCGTGTGGCAGAAGTGAAACCTAAAGTGGAAGCAATTCGTTCTGGC
  TCAGAAGAGGTGTTTAGGAGTGGTGCCCTCAAGCAGTTGCTGGAGGTGGTTTTGGCATTT
  GGAAATTATATGAATAAAGGTCAAAGAGGGAATGCATATGGATTCAAGATATCTAGCCTA
  AACAAAATTGCTGACACAAAATCCAGCATCGACAAAAACATTACCCTTTTGCACTATCTC
  ATCACTATTGTGGAAAATAAGTACCCCAGTGTTCTCAATCTAAATGAAGAATTGCGAGAT
  ATTCCTCAAGCTGCGAAAGTAAACATGACTGAGCTGGACAAAGAAATAAGTACCTTGAGA
  AGTGGCTTGAAAGCAGTAGAGACAGAGCTGGAATATCAGAAGTCTCAGCCCCCACAGCCC
  GGAGATAAGTTTGTGTCTGTTGTCAGCCAGTTCATCACAGTAGCCAGCTTCAGCTTCTCT
  GATGTTGAAGACCTTCTAGCAGAAGCTAAAGACCTGTTTACTAAAGCAGTGAAGCACTTT
  GGGGAAGAGGCTGGCAAAATACAACCAGATGAGTTCTTTGGCATTTTTGATCAATTTCTT
  CAAGCTGTGTCAGAAGCCAAACAAGAAAACGAAAATATGAGAAAGAAAAAGGAGGAAGAA
  GAACGTCGAGCTCGCATGGAAGCTCAGCTCAAAGAACAACGTGAAAGGGAACGTAAAATG
  AGAAAAGCTAAAGAGAATAGTGAAGAAAGCGGAGAGTTTGATGACCTTGTTTCAGCTTTA
  CGCTCAGGAGAAGTGTTTGACAAAGACCTTTCTAAATTGAAACGGAATCGCAAACGTATT
  ACCAACCAGATGACTGACAGCAGCAGAGAGAGACCAATCACAAAACTTAATTTCTAA
  
  Clone variation with respect to NM_014992.1
  1389 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014992 unedited
  ATTTTGTAATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGGCCTACGAGCGGC
  TGTGAAGGAAACTGTTTAACCGGATCCCATTGTACCCAGAGTGCAGAGCCGCCTTTCCAG
  CATGCAGGGGCTGCTCAGCGTTTAGTCACATCAAGAAATAGAACAGAATTCAGCCATGGC
  CCCAAGAAAGAGAGGTGGACGAGGTATTTCATTCATCTTTTGCTGTTTCCGAAATAATGA
  TCACCCAGAAATCACGTATCGGCTGCGAAATGATAGCAACTTTGCGCTTCAGACCATGGA
  ACCAGCATTGCCCATGCCCCCTGTGGAGGAGCTGGATGTCATGTTCAGTGAACTGGTGGA
  TGAACTGGACCTCACAGACAAACACAGAGAAGCCATGTTTGCACTTCCAGCTGAGAAAAA
  ATGGCAAATATACTGTAGCAAGAAAAAGGACCAGGAAGAAAACAAGGGAGCTACAAGTTG
  GCCTGAATTCTACATTGATCAGCTCAATTCCATGGCTGCTAGAAAATCTCTGCTGGCTTT
  AGAGAAGGAAGAAGAAGAAGAAAGAAGTAAAACTATAGAGAGTTTAAAGACAGCACTGAG
  GACAAAACCAATGAGGGTTTGTAACCAGATTCATCGACTTGGATGGCCTATCATGTATCC
  TCAACTTTCTAAAGACCATGGACTACGAGACCTCAGAGTCTCGATACATACTTCTCTCAT
  TGGCTGTATAAAGGGCGTAATGAACAACTCTCAAGGNCGGGCTCACGTNCTGGCTCATTC
  TGAGAGTATTAATGGTATTTGCTCAAAGTCTGAGCACAGAGAACATTANNAACGANGGTT
  GGCCGTGCTTGGAAATCTTNGGCCGCCGTGTTGCTGGGTTNCCGGGGGCACAAANAAAGT
  TCTCGCAGNCCATGCTGCACTACCAAAGTATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014992 unedited
  TAGCTTTGAACCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTAGTAATC
  TTTATCCCTTCAAAAAATCTTCCTTTAAGTCTTGGGTGAGATATTTTATTATTTTTAAGG
  TTTATATATCTATATTGTTAGAACAAGCATACTCTGGCAATGTTGTTCTTTAAAAAAAGC
  ATGGATTGCATTTATATAGTGTTTTTTTCCAAAAATCTGAAGAAATTATCAAGAGCACTT
  GGCAGAGATAACTTGATGAAATCAGGGGAAAAAATTGATGTCCAACTTTTAAATATACAA
  AAACTTGATTGACATTCTACATAATTCAAATTGTGTGCAAGATGTTCTCAATAAACATTT
  ACAGACTGTATCCCCACACCAATGTCAGAAATTCAAAGCTGGTCCTATTACTGATGTGAT
  ACATCAACATCACAATGTCAGCTATCAAAAATCATTTTAAATCTATGTTTTTCCAACAAC
  TCCAGTTCCATCAATGAAAATAATTTTAAAGGATATCAAGCTTGGAAACCATAAAATATT
  TTGCAAGCTTTTTTTGTTTGTTTTGTTTGTTTCTTCTGTTTTGCCTTTGAATCAGGGAAA
  TGTCTCTTATCTCTTTCAGGAGATAGTATTGTGCTTCTGAACAGTATGGCGTTCGGNGAA
  AGAAAATGAGCCCACTCAATGTGAATGCATACACGAAAAGTTTTGNAGCACATACATTAA
  CATAANATCTTTTAACCATACGTTAATAGGTCTCCAGACAACACTCAGACATCTATATAC
  AATTTTTTAATATATACACACATACATTTACATACACACGTTCACACATTCACTCACAGA
  TGAAGAANAAATGCCACTNTTTGAATGCCAAGNANTGTAATGAAAACTTCTAGTACTTTT
  AAAGGCTGCTATGAGCTCTAAAAAAGAATCATGAAATNAAATAAATTTNGGAATGGNCCT
  TCTGCTCTTGAAAATTGGGGGGAAACTAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014992
• Insert Size: 4600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014992.1, NP_055807.1
• RefSeq Size: 4256 bp
• RefSeq ORF: 3237 bp
• Locus ID: 23002
• UniProt ID: Q9Y4D1
• Cytogenetics: 14q23.1
• Domains: FH2
• Protein Pathways: Wnt signaling pathway
• Gene Summary: Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. The protein encoded by this gene contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. The protein encoded by this gene is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2012]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.