Asparagine synthetase (ASNS) (NM_133436) Human Untagged Clone

CAT#: SC120500

ASNS (untagged)-Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Asparagine synthetase
• Synonyms: ASNSD; TS11
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120500 sequence for NM_133436 edited (data generated by NextGen Sequencing)
  ATGTGTGGCATTTGGGCGCTGTTTGGCAGTGATGATTGCCTTTCTGTTCAGTGTCTGAGT
  GCTATGAAGATTGCACACAGAGGTCCAGATGCATTCCGTTTTGAGAATGTCAATGGATAC
  ACCAACTGCTGCTTTGGATTTCACCGGTTGGCGGTAGTTGACCCGCTGTTTGGAATGCAG
  CCAATTCGAGTGAAGAAATATCCGTATTTGTGGCTCTGTTACAATGGTGAAATCTACAAC
  CATAAGAAGATGCAACAGCATTTTGAATTTGAATACCAGACCAAAGTGGATGGTGAGATA
  ATCCTTCATCTTTATGACAAAGGAGGAATTGAGCAAACAATTTGTATGTTGGATGGTGTG
  TTTGCATTTGTTTTACTGGATACTGCCAATAAGAAAGTGTTCCTGGGTAGAGATACATAT
  GGAGTCAGACCTTTGTTTAAAGCAATGACAGAAGATGGATTTTTGGCTGTATGTTCAGAA
  GCTAAAGGTCTTGTTACATTGAAGCACTCCGCGACTCCCTTTTTAAAAGTGGAGCCTTTT
  CTTCCTGGACACTATGAAGTTTTGGATTTAAAGCCAAATGGCAAAGTTGCATCCGTGGAA
  ATGGTTAAATATCATCACTGTCGGGATGAACCCCTGCACGCCCTCTATGACAATGTGGAG
  AAACTCTTTCCAGGTTTTGAGATAGAAACTGTGAAGAACAACCTCAGGATCCTTTTTAAT
  AATGCTGTAAAGAAACGTTTGATGACAGACAGAAGGATTGGCTGCCTTTTATCAGGGGGC
  TTGGACTCCAGCTTGGTTGCTGCCACTCTGTTGAAGCAGCTGAAAGAAGCCCAAGTACAG
  TATCCTCTCCAGACATTTGCAATTGGCATGGAAGACAGCCCCGATTTACTGGCTGCTAGA
  AAGGTGGCAGATCATATTGGAAGTGAACATTATGAAGTCCTTTTTAACTCTGAGGAAGGC
  ATTCAGGCTCTGGATGAAGTCATATTTTCCTTGGAAACTTATGACATTACAACAGTTCGT
  GCTTCAGTAGGTATGTATTTAATTTCCAAGTATATTCGGAAGAACACAGATAGCGTGGTG
  ATCTTCTCTGGAGAAGGATCAGATGAACTTACGCAGGGTTACATATATTTTCACAAGGCT
  CCTTCTCCTGAAAAAGCCGAGGAGGAGAGTGAGAGGCTTCTGAGGGAACTCTATTTGTTT
  GATGTTCTCCGCGCAGATCGAACTACTGCTGCCCATGGTCTTGAACTGAGAGTCCCATTT
  CTAGATCATCGATTTTCTTCCTATTACTTGTCTCTGCCACCAGAAATGAGAATTCCAAAG
  AATGGGATAGAAAAACATCTCCTGAGAGAGACGTTTGAGGATTCCAATCTGATACCCAAA
  GAGATTCTCTGGCGACCAAAAGAAGCCTTCAGTGATGGAATAACTTCAGTTAAGAATTCC
  TGGTTTAAGATTTTACAGGAATACGTTGAACATCAGGTTGATGATGCAATGATGGCAAAT
  GCAGCCCAGAAATTTCCCTTCAATACTCCTAAAACCAAAGAAGGATATTACTACCGTCAA
  GTCTTTGAACGCCATTACCCAGGCCGGGCTGACTGGCTGAGCCATTACTGGATGCCCAAG
  TGGATCAATGCCACTGACCCTTCTGCCCGCACGCTGACCCACTACAAGTCAGCTGTCAAA
  GCTTAG
  
  Clone variation with respect to NM_133436.3
  629 t=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_133436 unedited
  TGTCTACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGATCACCCTGACCTGCTTA
  CGCCCAGATTTTCTTCAATCACATCTGAATAAATCACTTGAAGAAAGCTTATAGCTTCAT
  TGCACCATGTGTGGCATTTGGGCGCTGTTTGGCAGTGATGATTGCCTTTCTGTTCAGTGT
  CTGAGTGCTATGAAGATTGCACACAGAGGTCCAGATGCATTCCGTTTTGAGAATGTCAAT
  GGATACACCAACTGCTGCTTTGGATTTCACCGGTTGGCGGTAGTTGACCCGCTGTTTGGA
  ATGCAGCCAATTCGAGTGAAGAAATATCCGTATTTGTGGCTCTGTTACAATGGTGAAATC
  TACAACCATAAGAAGATGCAACAGCATTTTGAATTTGAATACCAGACCAAAGTGGATGGT
  GAGATAATCCTTCATCTTTATGACAAAGGAGGAATTGAGCAAACAATTTGTATGTTGGAT
  GGTGTGTTTGCATTTGTTTTACTGGATACTGCCAATAAGAAAGTGTTCCTGGGTAGAGAT
  ACATATGGAGTCAGACCTTTGTTTAAAGCAATGACAGAAGATGGATTTTTGGCTGTATGT
  TCAGAAGCTAAAGGTCTTGTTACATTGAAGCACTCCGCGACTCCCTTTTTAAAAGTGGAG
  CCTTTTCTTCCTGGACACTATGAAGTTNTGGATTTAAAGCCCAAATGGCAAGTTGCATCC
  GTGGAAATGGTTAAATATCATCACTGTCGGGATGAACCCCTGCACGCCCTTCTATGACAT
  GTGGAGAAACTCTTNTCCAGTTTTGAGATAGAAACTGTGAAGAACAACCTCAGNATCCTT
  TTTAAATATGCTGTAAAGAAACGTTTGATGACAGAAGAAGGATNGGCTGCCTTTATCAGG
  GGGCTNNGNACTCACCTTGNTGCTGCCCTCTGTTGAGCAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_133436 unedited
  CCGCGGCACGCTATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTAAAATTAGGACTTTTATTTTTTTCACACCCAAGTTAGCCTGAGTTGACTCTT
  ATTGGTCCCCTATTTACCCACAGTCCCCATCCAACACGAAAAAATATTTGCTTTCACATT
  ACAGCATAAAGACCACCTAAGCTTTGACAGCTGACTTGGAGGGGGTCAGCGGGCGGGCAA
  AAGGGTCAGGGGCATTGATCCACTTGGGCATCCAGTAATGGGTCAACCAGTCAGCCCGGC
  CTGGGTAATGGGGTTCAAAGACTTGACGGGAGTAATATCCTTCTTTGGGTTTAGGAGTAT
  TGAAGGGAAATTTCTGGGCTGGATTTGCCATCATTGGATCATCAACCTGATGGTCAACGG
  ATTCCTGGAAAATCTTAAACCAGGAATTCTTAACTGAAGTTATTCCATCACTGAAGGCTT
  CTTTTGGGCGCCAGAGAATCTCTTTGGGTATCAAATTGGAATCCTCAAACGTCTCTCTCA
  GGAGAAGTTTTTCTATCCCATTCTTTGGAATTCTCATTTCTGGGGGCAGAGACAAGTAAT
  AGGAAGAAAATCGATGATCCAAAAATGGGACTCTCAGTTCAAGACCATGGGCAGCAGTAG
  TTCGATCTGCGCGGAGAACATCAAACAAATAGAGTTCCCTCAGAAGCCTCTCACTCTCCT
  CCTCGGCTTTTTCAGGAGAAGGAGCCCTTGTGAAAATATATGGAACCCTGCGGTAAGTTC
  ATCTGATCCTTCTCCAGAGAAGACACCACGCTATCTGTGTTCCTCCGAATATACTCTGGA
  ATTAAATACCATACCTACTGGAGCACGAACTGTGGGAATGCCATAAGTCTCCAAGGGAAA
  AATGACTTCCATCCAAAGCCCTGATGCCCTCCTCANAGTAAAAAGGACTCCTTAAGGTCC
  CTTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_133436
• Insert Size: 1950 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133436.1, NP_597680.1
• RefSeq Size: 2348 bp
• RefSeq ORF: 1686 bp
• Locus ID: 440
• UniProt ID: P08243
• Cytogenetics: 7q21.3
• Domains: GATase_2, Asn_synthase
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Nitrogen metabolism
• Gene Summary: The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
  Transcript Variant: This variant (1) is the longest variant. Variants 1, 2, 3, and 7 encode the same isoform (a).