HINT3 (NM_138571) Human Untagged Clone

CAT#: SC120416

HINT3 (untagged)-Human histidine triad nucleotide binding protein 3 (HINT3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HINT3
• Synonyms: HINT4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120416 sequence for NM_138571 edited (data generated by NextGen Sequencing)
  ATGGCGGAGGAACAGGTGAACCGCAGCGCCGGCCTGGCCCCCGACTGTGAGGCCTCGGCG
  ACTGCAGAAACTACGGTTTCCTCAGTGGGGACCTGTGAAGCCGCTGCCAAGTCACCAGAG
  CCCAAGGACTACGACAGCACCTGCGTGTTCTGCCGGATCGCGGGGCGGCAGGACCCGGGC
  ACCGAACTCCTGCACTGCGAGAATGAGGACCTAATTTGCTTCAAAGATATCAAACCAGCA
  GCAACTCATCATTATCTTGTGGTGCCAAAGAAGCATATTGGAAACTGCAGAACTCTAAGG
  AAAGATCAAGTAGAACTGGTTGAGAACATGGTAACTGTTGGAAAAACCATTCTTGAAAGA
  AATAATTTCACTGACTTCACGAATGTGAGGATGGGTTTTCATATGCCACCATTCTGTTCC
  ATTTCCCACTTGCACCTTCATGTTCTGGCACCAGTGGATCAGCTTGGCTTCTTATCCAAG
  TTGGTTTATAGAGTCAATTCCTATTGGTTTATCACAGCTGATCACTTGATTGAAAAACTA
  AGAACATGA
  
  Clone variation with respect to NM_138571.4
  107 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138571 unedited
  NGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGTAGCCC
  TGGAGAGGCCGAGGCTCTAGGCCGCGAGGGGCGGGTGCAATGGCGGAGGAACAGGTGAAC
  CGCAGCGCCGGCCTGGCCCCCGACTGTGAGGCCTCGGCGACTGCAGAAACTACGGTTTCC
  TCAGTGGGGACCTGTGAAGCCGCTGCCAAGTCACCAGAGCCCAAGGACTACGACAGCACC
  TGCGTGTTCTGCCGGATCGCGGGGCGGCAGGACCCGGGCACCGAACTCCTGCACTGCGAG
  AATGAGGACCTAATTTGCTTCAAAGATATCAAACCAGCAGCAACTCATCATTATCTTGTG
  GTGCCAAAGAAGCATATTGGAAACTGCAGAACTCTAAGGAAAGATCAAGTAGAACTGGTT
  GAGAACATGGTAACTGTTGGAAAAACCATTCTTGAAAGAAATAATTTCACTGACTTCACG
  AATGTGAGGATGGGTTTTCATATGCCACCATTCTGTTCCATTTCCCACTTCCCCTTTTCA
  TGTTCTGGCACCAGTGGATCAGCTTGGCTTCTTATCCAAGTTGGTTTATAGAGTCAATTC
  CTATTGGTTTATCACAGCTGATCACTTGATTGAAAAACTAAGAACATGAAAATGTCAAGA
  GTGGNAAGATTTTTCTAATCTTGGTTCACATGAAGTGGTAATTTAAGTCCCTTTTAAGTC
  TAATTGCATTTTAAGAATTGNTGGGTTTTATGAGAGGCTGTTACTTAGTGGCCTTAAATC
  TTTTCTGAATGTCTGTTTCCTAAGATCTGTGAACCAGTTATGTGAAATATTTTGGTACTG
  ACTTTGTTCATGGGGTACTGATTANGGATTTATATATATGATACTATAGATAAAATCTAT
  TTAGACANATCTGTTATCACAGGGCCTGGATTTTTTTAAGTAAAATTTCATTCTAGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_138571
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138571.3, NP_612638.2
• RefSeq Size: 1504 bp
• RefSeq ORF: 549 bp
• Locus ID: 135114
• UniProt ID: Q9NQE9
• Cytogenetics: 6q22.32
• Domains: HIT
• Gene Summary: Histidine triad proteins, such as HINT3, are nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides (Brenner, 2002 [PubMed 12119013]).[supplied by OMIM, Mar 2008]