LARP1 (NM_033551) Human Untagged Clone

CAT#: SC120115

LARP1 (untagged)-Human La ribonucleoprotein domain family, member 1 (LARP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LARP1
• Synonyms: Lar1; LARP; Lhp1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033551, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCACTCAGGTGGAGCCGCTGCTGCCTGGGGGCGCCACGCTCTTGCAGGCCGAAGAGCACGGGGGGC
  TGGTGAGGAAGAAGCCGCCGCCGGCGCCCGAGGGCAAGGGCGAGCCCGGGCCAAACGACGTCCGCGGGGG
  GGAGCCGGACGGCAGCGCTCGGAGACCCCGGCCGCCCTGCGCCAAGCCGCACAAGGAGGGCACCGGGCAG
  CAGGAGCGCGAGAGCCCGCGGCCGCTGCAGCTGCCCGGCGCCGAAGGCCCGGCCATCAGCGACGGGGAGG
  AGGGCGGCGGCGAGCCAGGCGCTGGCGGAGGAGCTGCCGGAGCCGCGGGCGCGGGGCGCCGGGACTTCGT
  GGAAGCCCCCCCGCCCAAGGTGAACCCGTGGACTAAGAACGCATTGCCGCCGGTCCTGACCACCGTGAAC
  GGACAGTCCCCCCCAGAACACTCTGCTCCAGCCAAGGTGGTGAGGGCAGCTGTTCCTAAACAGCGCAAAG
  GCAGCAAGGTTGGTGACTTTGGAGATGCAATCAATTGGCCCACACCTGGAGAGATAGCCCACAAGAGTGT
  TCAGCCACAGTCCCACAAGCCTCAGCCTACCCGTAAACTGCCACCCAAGAAGGACATGAAGGAACAGGAG
  AAAGGAGAAGGGAGTGATAGTAAGGAGAGTCCAAAAACCAAATCAGATGAATCAGGGGAGGAAAAGAATG
  GAGATGAGGATTGCCAGCGAGGCGGGCAGAAGAAGAAAGGAAACAAACACAAGTGGGTTCCATTACAAAT
  AGACATGAAGCCTGAAGTGCCCAGAGAGAAACTGGCTTCACGCCCCACTCGCCCACCGGAGCCTAGACAC
  ATACCTGCCAATCGCGGAGAGATCAAAGGGTCTGAGTCTGCCACCTACGTGCCCGTGGCCCCCCCCACCC
  CAGCCTGGCAACCAGAGATCAAACCGGAGCCTGCCTGGCACGACCAGGATGAGACATCGAGTGTGAAGAG
  TGATGGGGCTGGTGGGGCGCGGGCTTCCTTCCGTGGCCGTGGACGGGGGCGTGGTCGCGGCCGGGGACGC
  GGCCGGGGTGGCACTCGAACCCATTTTGACTACCAGTTTGGCTACCGAAAGTTTGATGGTGTGGAGGGGC
  CTCGTACGCCCAAGTACATGAACAACATCACCTACTACTTTGACAATGTCAGCAGCACCGAGCTTTACAG
  TGTGGATCAGGAACTGCTCAAAGACTACATCAAGCGCCAGATTGAATACTACTTCAGCGTGGACAATTTA
  GAGCGAGACTTCTTCCTGCGAAGGAAAATGGATGCTGATGGTTTCCTACCCATCACCCTTATTGCTTCCT
  TCCACCGAGTGCAGGCCCTTACCACTGACATTTCACTCATCTTTGCGGCCCTAAAGGACAGCAAGGTGGT
  GGAGATCGTTGATGAGAAAGTTCGTAGGAGGGAGGAACCAGAAAAGTGGCCTCTTCCCCCAATAGTGGAT
  TATTCACAGACTGATTTCTCCCAGCTTCTCAACTGCCCTGAATTTGTTCCCCGTCAGCACTACCAAAAGG
  AGACAGAGTCGGCACCTGGCTCTCCTCGTGCAGTCACCCCAGTGCCAACCAAAACAGAGGAGGTCAGCAA
  CCTAAAGACACTACCCAAGGGCCTGTCTGCCAGCCTGCCTGACCTGGATTCTGAGAACTGGATTGAAGTG
  AAGAAGAGGCCTCGGCCATCCCCAGCACGGCCCAAGAAGTCAGAGGAGTCCAGATTTTCCCACCTGACCT
  CTCTGCCTCAGCAGCTGCCTTCCCAGCAGCTGATGTCCAAGGATCAGGATGAGCAAGAGGAACTGGATTT
  TCTGTTTGACGAGGAGATGGAGCAGATGGATGGGCGGAAGAACACCTTCACTGCCTGGTCTGATGAGGAA
  TCTGACTATGAGATTGATGACAGGGATGTCAACAAGATCCTCATTGTCACCCAGACACCACATTACATGC
  GCCGGCACCCAGGGGGGGACCGCACAGGCAACCACACCTCGCGTGCCAAGATGAGCGCCGAACTGGCCAA
  GGTCATTAATGATGGCCTCTTCTACTATGAGCAGGACCTGTGGGCTGAAAAGTTTGAACCTGAGTATTCC
  CAGATCAAGCAAGAAGTCGAGAACTTCAAAAAGGTCAATATGATCAGCCGGGAGCAGTTTGACACACTGA
  CCCCTGAGCCCCCTGTGGATCCCAACCAGGAAGTTCCTCCTGGGCCACCTCGGTTCCAGCAAGTTCCTAC
  GGATGCCCTGGCCAACAAGTTGTTTGGTGCTCCTGAGCCCTCCACCATCGCCCGCTCTCTACCAACCACT
  GTCCCAGAGTCACCAAACTACCGCAACACCAGGACCCCTCGCACTCCCCGGACACCACAGCTCAAAGACT
  CAAGCCAGACATCACGGTTTTACCCAGTGGTGAAAGAAGGACGGACACTGGATGCCAAGATGCCTCGAAA
  AAGAAAGACAAGACACAGTTCAAACCCACCCTTGGAGAGCCATGTGGGCTGGGTGATGGATTCCCGTGAG
  CACAGGCCCCGTACTGCTTCCATCAGCTCCAGCCCCTCAGAAGGGACGCCTACAGTTGGCAGCTATGGCT
  GTACCCCTCAGTCATTGCCCAAGTTCCAGCATCCTTCCCATGAACTGCTCAAGGAAAATGGCTTCACACA
  ACACGTCTACCATAAGTATCGTAGGCGCTGCCTTAATGAGCGGAAACGCTTGGGCATTGGCCAGTCTCAG
  GAGATGAACACACTCTTCCGCTTCTGGTCCTTCTTCCTCCGAGATCACTTCAACAAAAAGATGTATGAGG
  AGTTCAAGCAGCTGGCTCTGGAGGACGCCAAAGAAGGCTACAGATATGGTTTGGAGTGCCTTTTTCGATA
  CTACAGTTATGGCCTGGAAAAGAAGTTCCGGCTGGACATATTCAAGGATTTTCAGGAGGAAACGGTGAAG
  GACTATGAAGCTGGCCAACTGTATGGGCTGGAGAAGTTCTGGGCCTTCTTGAAATATTCCAAAGCCAAAA
  ATTTGGACATTGACCCCAAACTGCAAGAATACCTCGGCAAATTCCGACGTCTTGAAGACTTCCGAGTAGA
  TCCCCCCATGGGTGAGGAGGGCAACCACAAGCGACACTCAGTGGTAGCAGGAGGTGGCGGCGGTGAGGGC
  AGGAAGCGGTGCCCCTCCCAGTCTTCCAGCAGGCCTGCTGCCATGATCAGCCAACCCCCTACACCACCCA
  CCGGCCAGCCTGTCCGGGAAGATGCCAAATGGACAAGCCAGCACTCGAACACACAGACTTTGGGAAAGTG
  A
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033551 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGGAGCGGGAATC
  TGAAATACCCAAATGCCTGTTTTCCTCGGTGGAGTCAACCCGAAGAGCTCCCACCTTCTC
  TGGATGTGCCTGGGCTTGGACTGGCTAGAATCTTTCTCTGGACTGTTGCATGTACAGTGC
  CTCCATCCTGGAGGCAAGAGAGTTGGGAGTGGCTCGAATCAGAGCCGTGCCCAAGATATC
  CCTGCTGTTGCATCGTTTGAAGCTGACGTCCTGTGTCTGTACACTGCTGCCACTGTTGTG
  TCCTCGCTCTGCTTGCTGTTGCCTCACGCCAGGCCCCGTCCTGCCGTGACACCCTTCATC
  CTACCCTTGGAACCCCAAGGCCAAGTTGGTTCAAACTGTTGGAGAACAGAGTTGGCCTGC
  ATCTGGAACACACTTGTCCTCAGCTTACCATCTCCTCACACCCCAGAGTGGAAAGGTGAA
  CACCTGCAGCTGAGGCTTGGAAACGTTTCTTGTGTTGCCCTGAAAAATCTTTGAGACCTC
  AGGGAGGCTCTGTCTCTCTTAAAAGGTGGAGAAAGATGCCATTCTCTCCCTAAGGTCTGG
  TGGAGTCTCCCCATCTTGCATACCCTTCTGCAAGCCATCTATCTCTGCTCACTCTCCAAT
  TGACCCGCCTGGGAACAAGGGATGAGGAGGAGTTGGGGGCTGGGGGGAATCCTGCCAGTT
  GGTGAAGCCCTGTGGCAGGAAGTATATGTGGACATAGAGTATACCTGATTCTCTTTCTTC
  ANCCCTGACTGCTTGGGTNGNGCTGTGAATGATAATGGAATGGNCTGAGTCTGCTGNTGT
  CAAAAGGCAGGAGGNTGATGAAGGACTGACCACATGGACTGGNATGGGTGTCGGNTTGGG
  CATGACTGCACGTCACTCTAATGGNGACTGGCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033551 unedited
  NCGTATTCTATGNACCGCGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTGCCTT
  TTCAATGTTTATTTTTATACATTTTTTTGTATTAAAAAGAAAAGCTAATTACCACAAATT
  ACAAAGGACTAAAGCAGGACTAGAATAATGAATGAATCACTTCAGCCTGGAAAGCAGATA
  CTCTCAATAATATTAATGTTATAATACAAGCTCATTCAAGTATTTTACATTTTTTTGTCC
  TTTTAAATATGATATCCTAGCACATAGTAAAGATAATGTACTACGAGCGTAAGGTGGAAC
  CTTCTCCGCAAAGCCAGATGACAAGTTTTCCTCTCCAGTGAGAAATGGGCTCCAAGTTCT
  TATCTTCTCTGCCACTCAGCATGGCTGAAGGGGGAGAGGGTGGGTGTTAGGGAAAATCCA
  GGGACCCTTCCACACAGGAGGTACTCTGGGGTTCCATTCAGGAGGGTGCCAGATAATTCA
  GATCACTCTACCCAGGCTTTTTGGCAAAAAACAACCCTCACCCTCCGTAACTAGTCATCT
  TCCTTGTCCCTCCTCCTCACTTCCCTTCCAGGGCCTTCTAAGCAGCTTACTTTCTTTTGT
  AAAATCAATCACCAGGAAAGGGGAAAAAAAAAAAAAAAGAGCTACAATGGAACAAAACAA
  AAACATTTAACAACTTTGACACAGGGGCTCAGCACCTCTATCTGAGCCCCAGGGGTCCAG
  GCTGGGGCACGGCTGGATGGCCCTTCTCTACTGGTAGTGCCTTTGCTCCCAAGTGCAGGA
  AGAGGGCTAAAGAGAAACCTGCCCCTTAACAGGGCTGAGGCACACAGACCTGCTGATGTC
  TGATGCCCAAAAGTTATGTTGGCCATGGACCAACCTTGGAAGGGATAGGATGAACAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033551
• Insert Size: 2750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033551.1, NP_291029.1
• RefSeq Size: 7280 bp
• RefSeq ORF: 162 bp
• Locus ID: 23367
• Cytogenetics: 5q33.2
• Gene Summary: RNA-binding protein that regulates the translation of specific target mRNA species downstream of the mTORC1 complex, in function of growth signals and nutrient availability (PubMed:20430826, PubMed:23711370, PubMed:24532714, PubMed:25940091, PubMed:28650797, PubMed:28673543, PubMed:29244122). Interacts on the one hand with the 3' poly-A tails that are present in all mRNA molecules, and on the other hand with the 7-methylguanosine cap structure of mRNAs containing a 5' terminal oligopyrimidine (5'TOP) motif, which is present in mRNAs encoding ribosomal proteins and several components of the translation machinery (PubMed:23711370, PubMed:25940091, PubMed:28650797, PubMed:29244122, PubMed:26206669, PubMed:28379136). The interaction with the 5' end of mRNAs containing a 5'TOP motif leads to translational repression by preventing the binding of EIF4G1 (PubMed:25940091, PubMed:28650797, PubMed:29244122, PubMed:28379136). When mTORC1 is activated, LARP1 is phosphorylated and dissociates from the 5' untranslated region (UTR) of mRNA (PubMed:25940091, PubMed:28650797). Does not prevent binding of EIF4G1 to mRNAs that lack a 5'TOP motif (PubMed:28379136). Interacts with the free 40S ribosome subunit and with ribosomes, both monosomes and polysomes (PubMed:20430826, PubMed:24532714, PubMed:25940091, PubMed:28673543). Under normal nutrient availability, interacts primarily with the 3' untranslated region (UTR) of mRNAs encoding ribosomal proteins and increases protein synthesis (PubMed:23711370, PubMed:28650797). Associates with actively translating ribosomes and stimulates translation of mRNAs containing a 5'TOP motif, thereby regulating protein synthesis, and as a consequence, cell growth and proliferation (PubMed:20430826, PubMed:24532714). Stabilizes mRNAs species with a 5'TOP motif, which is required to prevent apoptosis (PubMed:20430826, PubMed:23711370, PubMed:25940091, PubMed:28673543).[UniProtKB/Swiss-Prot Function]