Mitofusin 1 (MFN1) (NM_033540) Human Untagged Clone

CAT#: SC120111

MFN1 (untagged)-Human mitofusin 1 (MFN1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Mitofusin 1
• Synonyms: hfzo1; hfzo2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120111 sequence for NM_033540 edited (data generated by NextGen Sequencing)
  ATGGCAGAACCTGTTTCTCCACTGAAGCACTTTGTGCTGGCTAAGAAGGCGATTACTGCA
  ATCTTTGACCAGTTACTGGAGTTTGTTACTGAAGGATCACATTTTGTTGAAGCAACATAT
  AAGAATCCGGAACTTGATCGAATAGCCACTGAAGATGATCTGGTAGAAATGCAAGGATAT
  AAAGACAAGCTTTCCATCATTGGTGAGGTGCTATCTCGGAGACACATGAAGGTGGCATTT
  TTTGGCAGGACAAGCAGTGGGAAGAGCTCTGTTATCAATGCAATGTTGTGGGATAAAGTT
  CTCCCTAGTGGGATTGGCCATATAACCAATTGCTTCCTAAGTGTTGAAGGAACTGATGGA
  GATAAAGCCTATCTTATGACAGAAGGATCAGATGAAAAAAAGAGTGTGAAGACAGTTAAT
  CAACTGGCCCATGCCCTTCACATGGACAAAGATTTGAAAGCTGGCTGTCTTGTACGTGTG
  TTTTGGCCAAAAGCAAAATGTGCCCTCTTGAGAGATGACCTGGTGTTAGTAGACAGTCCA
  GGCACAGATGTCACTACAGAGCTGGATAGCTGGATTGATAAGTTTTGCCTAGATGCTGAT
  GTCTTTGTTTTGGTCGCAAACTCTGAATCAACACTAATGAATACGGAAAAACACTTTTTT
  CACAAGGTGAATGAGCGGCTTTCCAAGCCTAATATTTTCATTCTCAATAATCGTTGGGAT
  GCCTCTGCATCAGAGCCAGAATATATGGAAGACGTACGCAGACAGCACATGGAAAGATGC
  CTGCATTTCTTGGTGGAGGAGCTCAAAGTTGTAAATGCTTTAGAAGCACAGAATCGTATC
  TTCTTTGTTTCAGCAAAGGAAGTTCTTAGTGCTAGAAAGCAAAAAGCACAGGGGATGCCA
  GAAAGTGGTGTGGCACTTGCTGAAGGATTTCATGCAAGATTACAGGAATTTCAGAATTTT
  GAACAAATCTTTGAGGAGTGTATCTCGCAGTCAGCAGTGAAAACAAAGTTCGAACAGCAC
  ACTATCAGAGCTAAACAGATACTAGCTACTGTGAAAAACATAATGGATTCAGTAAACCTG
  GCAGCTGAAGATAAAAGGCATTATTCAGTGGAAGAGAGGGAAGACCAAATTGATAGACTG
  GACTTTATTCGAAACCAGATGAACCTTTTAACACTGGATGTTAAGAAAAAAATCAAGGAG
  GTTACCGAGGAGGTGGCAAACAAAGTTTCATGTGCAATGACAGATGAAATTTGTCGACTG
  TCTGTTTTGGTTGATGAATTTTGTTCAGAGTTTCATCCTAATCCAGATGTATTAAAAATA
  TATAAAAGTGAATTAAATAAGCACATAGAGGATGGTATGGGAAGAAATTTGGCTGATCGA
  TGCACCGATGAAGTAAACGCCTTAGTGCTTCAGACCCAGCAAGAAATTATTGAAAATTTG
  AAGCCATTACTTCCAGCTGGTATACAGGATAAACTACATACACTGATCCCTTGCAAGAAA
  TTTGATCTCAGTTATAATCTAAATTACCACAAGTTATGTTCAGATTTTCAAGAGGATATT
  GTATTTCGTTTTTCCCTGGGCTGGTCTTCCCTTGTACATCGATTTTTGGGCCCTAGAAAT
  GCTCAAAGGGTGCTCCTAGGATTATCAGAGCCTATCTTTCAGCTCCCTAGATCTTTAGCT
  TCTACTCCCACTGCTCCTACCACTCCAGCAACGCCAGATAATGCATCACAGGAAGAACTC
  ATGATTACATTAGTAACAGGATTGGCGTCCGTTACATCTAGAACTTCTATGGGCATCATT
  ATTGTTGGAGGAGTGATTTGGAAAACTATAGGCTGGAAACTCCTATCTGTTTCATTAACT
  ATGTATGGAGCTTTGTATCTTTATGAAAGACTGAGCTGGACCACCCATGCCAAGGAGCGA
  GCCTTTAAACAGCAGTTTGTAAACTATGCAACTGAAAAACTGAGGATGATTGTTAGCTCC
  ACGAGTGCAAACTGCAGTCACCAAGTAAAACAACAAATAGCTACCACTTTTGCTCGCCTG
  TGCCAACAAGTTGATATTACTCAAAAACAGCTGGAAGAAGAAATTGCTAGATTACCCAAA
  GAAATAGATCAGTTGGAGAAAATACAAAACAATTCAAAGCTCTTAAGAAATAAAGCTGTT
  CAACTTGAAAATGAGCTGGAGAATTTTACTAAGCAGTTTCTACCTTCAAGCAATGAAGAA
  TCCTAA
  
  Clone variation with respect to NM_033540.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033540 unedited
  TTTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGGAAGATGAGGCAG
  TTTGGCATCTGTGGCCGAGTTGCTGTTGCCGGGTGATAGTTGGAGCGGAGACTTAGCATA
  ATGGCAGAACCTGTTTCTCCACTGAAGCACTTTGTGCTGGCTAAGAAGGCGATTACTGCA
  ATCTTTGACCAGTTACTGGAGTTTGTTACTGAAGGATCACATTTTGTTGAAGCAACATAT
  AAGAATCCGGAACTTGATCGAATAGCCACTGAAGATGATCTGGTAGAAATGCAAGGATAT
  AAAGACAAGCTTTCCATCATTGGTGAGGTGCTATCTCGGAGACACATGAAGGTGGCATTT
  TTTGGCAGGACAAGCAGTGGGAAGAGCTCTGTTATCAATGCAATGTTGTGGGATAAAGTT
  CTCCCTAGTGGGATTGGCCATATAACCAATTGCTTCCTAAGTGTTGAAGGAACTGATGGA
  GATAAAGCCTATCTTATGACAGAAGGATCAGATGAAAAAAAGAGTGTGAAGACAGTTAAT
  CAACTGGCCCATGCCCTTCACATGGACAAAGATTTGAAAGCTGGCTGTCTTGTACGTGTG
  TTTTGGCCAAAAGCAAAATGTGCCCTCTTGAGAGATGACCTGNTGTTAGTAGACAGTCCA
  GGCACAGATGTCACTACAGAGCTGGATAGCTGGATTGATAAGTTTGCCTAGATGCTGATG
  TCTTTGTTTTGGTCGCAAACTCTGAATCAACACTAATGAATACGGAAAAACACTTTTTTC
  ACAAGGTGAATGAGCGGCTCTCCAAGCCTAAATTTTCATTCTCAATAATCGTGGGATGCC
  TCTGCATCAGAGCCAGAATATATGGAGACGTACGCACACGCACATGGAAAGAGCCTGCAT
  TCTTGGTGAGAGCTCAAATTGTAATGCTTTAAAGCCAAAATCTTCTTCTGGTTTACAAAG
  AGATCTAAGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033540 unedited
  GCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTGTATTTGCATATTCTTATTTAAA
  GTTCTGGATGCTGGTAAGTCCAAGAGCATGGCACTGTCATCTGGCGAGGCCTTTCTTGCT
  GTGTTGTAAAATCATGGCAGAAGGCAAAGGGGAAGCAGGCGTCTTCACATGGCTGGAGCT
  GAGCGTGGTGGCAGGCACCTGTAATTCCAGCTACTCAAGAGGCTGAGGCAGGAGAATTGC
  TTGAACCTGGGAAGCGGAGGTTGCAGTGAGCCGAGATTGCGCCACCGCACTCCAGCCTGG
  GTGACAGAGCAAGACTCCGTAGAAGAATGAATGAATGGAGCTCCCAGAGACTGTCCCTTA
  GAGTTCTGCATTCATTTGCTCCCTCATTAATTGAAGAAAATTGGTGGGTGCATGTGCTGT
  GCTAAGACTGTGGCTCCTAAGTAGAGTCTGAAGTCTGGTCTATGTGGCTTGGTATCAGTT
  CAAAGCAATCGTTTCCATCAGAAAACTATTTGCCAAATGGATAAACCAACTGTGGTACTT
  CCATACCATGGAATGCTATGGCACTATGGATTACAGGGTAGCTCACTGAGTACAGCATTC
  AGATTCATCCAATGTAAGACCAAGAGCAGAGCTTCTCTGAACTTGATCATAATGAAGCAA
  AACACCAAGTTTCGTCTCCTCCTATCATGGTCACCAAAGCCATTCTCTATTGTTAAGATT
  CTTCTTTGCTTGGAAGTACAAACTGCTTATTAAAATTCTCCCACTCTTTTTCAAGTTGAC
  AGCTTTAATTCTTAAAACTTTGAATGTTTTGGATTTTCCCAACGAACATTTCTTTGGTAT
  CTAACATTTTTTTTTCACTGGTTTGAGATAACACTTTGTGGCCAGCAACAAAAGGGATCC
  TTTTGGGGTTACTTGGGACGGATTTGCCCCCGGACCAACAAATTCTATTTTTCATGCTAG
  TTCAAACGCGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033540
• Insert Size: 3030 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033540.2, NP_284941.2
• RefSeq Size: 3527 bp
• RefSeq ORF: 2226 bp
• Locus ID: 55669
• UniProt ID: Q8IWA4
• Cytogenetics: 3q26.33
• Domains: fzo_mitofusin
• Gene Summary: The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. [provided by RefSeq, Jul 2008]