Jagged1 (JAG1) (NM_000214) Human Untagged Clone

CAT#: SC120058

JAG1 (untagged)-Human jagged 1 (JAG1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Jagged1
• Synonyms: AGS; AGS1; AHD; AWS; CD339; DCHE; HJ1; JAGL1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000214 edited
  GAATTCGGCACGAGCTTCTCCGAGAGCCGGGCGGGCACGCGTCATTGTGTTACCTGCGGC
  CGGCCCGCGAGCTAGGCTGGTTTTTTTTTTTCTCCCCTCCCTCCCCCCTTTTTCCATGCA
  GCTGATCTAAAAGGGAATAAAAGGCTGCGCATAATCATAATAATAAAAGAAGGGGAGCGC
  GAGAGAAGGAAAGAAAGCCGGGAGGTGGAAGAGGAGGGGGAGCGTCTCAAAGAAGCGATC
  AGAATAATAAAAGGAGGCCGGGCTCTTTGCCTTCTGGAACGGGCCGCTCTTGAAAGGGCT
  TTTGAAAAGTGGTGTTGTTTTCCAGTCGTGCATGCTCCAATCGGCGGAGTATATTAGAGC
  CGGGACGCGGCGGCCGCAGGGGCAGCGGCGACGGCAGCACCGGCGGCAGCACCAGCGCGA
  ACAGCAGCGGCGGCGTCCCGAGTGCCCGCGGCGCGCGGCGCAGCGATGCGTTCCCCACGG
  ACGCGCGGCCGGTCCGGGCGCCCCCTAAGCCTCCTGCTCGCCCTGCTCTGTGCCCTGCGA
  GCCAAGGTGTGTGGGGCCTCGGGTCAGTTCGAGTTGGAGATCCTGTCCATGCAGAACGTG
  AACGGGGAGCTGCAGAACGGGAACTGCTGCGGCGGCGCCCGGAACCCGGGAGACCGCAAG
  TGCACCCGCGACGAGTGTGACACATACTTCAAAGTGTGCCTCAAGGAGTATCAGTCCCGC
  GTCACGGCCGGGGGGCCCTGCAGCTTCGGCTCAGGGTCCACGCCTGTCATCGGGGGCAAC
  ACCTTCAACCTCAAGGCCAGCCGCGGCAACGACCGCAACCGCATCGTGCTGCCTTTCAGT
  TTCGCCTGGCCGAGGTCCTATACGTTGCTTGTGGAGGCGTGGGATTCCAGTAATGACACC
  GTTCAACCTGACAGTATTATTGAAAAGGCTTCTCACTCGGGCATGATCAACCCCAGCCGG
  CAGTGGCAGACGCTGAAGCAGAACACGGGCGTTGCCCACTTTGAGTATCAGATCCGCGTG
  ACCTGTGATGACTACTACTATGGCTTTGGCTGCAATAAGTTCTGCCGCCCCAGAGATGAC
  TTCTTTGGACACTATGCCTGTGACCAGAATGGCAACAAAACTTGCATGGAAGGCTGGATG
  GGCCCCGAATGTAACAGAGCTATTTGCCGACAAGGCTGCAGTCCTAAGCATGGGTCTTGC
  AAACTCCCAGGTGACTGCAGGTGCCAGTACGGCTGGCAAGGCCTGTACTGTGATAAGTGC
  ATCCCACACCCGGGATGCGTCCACGGCATCTGTAATGAGCCCTGGCAGTGCCTCTGTGAG
  ACCAACTGGGGCGGCCAGCTCTGTGACAAAGATCTCAATTACTGTGGGACTCATCAGCCG
  TGTCTCAATGGGGGAACTTGTAGCAACACAGGCCCTGACAAATATCAGTGTTCCTGCCCT
  GAGGGGTATTCAGGACCCAACTGTGAAATTGCTGAGCACGCCTGCCTCTCTGATCCCTGT
  CACAACAGAGGCAGCTGTAAGGAGACCTCCCTGGGCTTTGAGTGTGAGTGTTCCCCAGGC
  TGGACCGGCCCCACATGCTCTACAAACATTGATGACTGTTCTCCTAATAACTGTTCCCAC
  GGGGGCACCTGCCAGGACCTGGTTAACGGATTTAAGTGTGTGTGCCCCCCACAGTGGACT
  GGGAAAACGTGCCAGTTAGATGCAAATGAATGTGAGGCCAAACCTTGTGTAAACGCCAAA
  TCCTGTAAGAATCTCATTGCCAGCTACTACTGCGACTGTCTTCCCGGCTGGATGGGTCAG
  AATTGTGACATAAATATTAATGACTGCCTTGGCCAGTGTCAGAATGACGCCTCCTGTCGG
  GATTTGGTTAATGGTTATCGCTGTATCTGTCCACCTGGCTATGCAGGCGATCACTGTGAG
  AGAGACATCGATGAATGTGCCAGCAACCCCTGTTTGAATGGGGGTCACTGTCAGAATGAA
  ATCAACAGATTCCAGTGTCTGTGTCCCACTGGTTTCTCTGGAAACCTCTGTCAGCTGGAC
  ATCGATTATTGTGAGCCTAATCCCTGCCAGAACGGTGCCCAGTGCTACAACCGTGCCAGT
  GACTATTTCTGCAAGTGCCCCGAGGACTATGAGGGCAAGAACTGCTCACACCTGAAAGAC
  CACTGCCGCACGACCCCCTGTGAAGTGATTGACAGCTGCACAGTGGCCATGGCTTCCAAC
  GACACACCTGAAGGGGTGCGGTATATTTCCTCCAACGTCTGTGGTCCTCACGGGAAGTGC
  AAGAGTCAGTCGGGAGGCAAATTCACCTGTGACTGTAACAAAGGCTTCACGGGAACATAC
  TGCCATGAAAATATTAATGACTGTGAGAGCAACCCTTGTAGAAACGGTGGCACTTGCATC
  GATGGTGTCAACTCCTACAAGTGCATCTGTAGTGACGGCTGGGAGGGGGCCTACTGTGAA
  ACCAATATTAATGACTGCAGCCAGAACCCCTGCCACAATGGGGGCACGTGTCGCGACCTG
  GTCAATGACTTCTACTGTGACTGTAAAAATGGGTGGAAAGGAAAGACCTGCCACTCACGT
  GACAGTCAGTGTGATGAGGCCACGTGCAACAACGGTGGCACCTGCTATGATGAGGGGGAT
  GCTTTTAAGTGCATGTGTCCTGGCGGCTGGGAAGGAACAACCTGTAACATAGCCCGAAAC
  AGTAGCTGCCTGCCCAACCCCTGCCATAATGGGGGCACATGTGTGGTCAACGGCGAGTCC
  TTTACGTGCGTCTGCAAGGAAGGCTGGGAGGGGCCCATCTGTGCTCAGAATACCAATGAC
  TGCAGCCCTCATCCCTGTTACAACAGCGGCACCTGTGTGGATGGAGACAACTGGTACCGG
  TGCGAATGTGCCCCGGGTTTTGCTGGGCCCGACTGCAGAATAAACATCAATGAATGCCAG
  TCTTCACCTTGTGCCTTTGGAGCGACCTGTGTGGATGAGATCAATGGCTACCGGTGTGTC
  TGCCCTCCAGGGCACAGTGGTGCCAAGTGCCAGGAAGTTTCAGGGAGACCTTGCATCACC
  ATGGGGAGTGTGATACCAGATGGGGCCAAATGGGATGATGACTGTAATACCTGCCAGTGC
  CTGAATGGACGGATCGCCTGCTCAAAGGTCTGGTGTGGCCCTCGACCTTGCCTGCTCCAC
  AAAGGGCACAGCGAGTGCCCCAGCGGGCAGAGCTGCATCCCCATCCTGGACGACCAGTGC
  TTCGTCCACCCCTGCACTGGTGTGGGCGAGTGTCGGTCTTCCAGTCTCCAGCCGGTGAAG
  ACAAAGTGCACCTCTGACTCCTATTACCAGGATAACTGTGCGAACATCACATTTACCTTT
  AACAAGGAGATGATGTCACCAGGTCTTACTACGGAGCACATTTGCAGTGAATTGAGGAAT
  TTGAATATTTTGAAGAATGTTTCCGCTGAATATTCAATCTACATCGCTTGCGAGCCTTCC
  CCTTCAGCGAACAATGAAATACATGTGGCCATTTCTGCTGAAGATATACGGGATGATGGG
  AACCCGATCAAGGAAATCACTGACAAAATAATCGATCTTGTTAGTAAACGTGATGGAAAC
  AGCTCGCTGATTGCTGCCGTTGCAGAAGTAAGAGTTCAGAGGCGGCCTCTGAAGAACAGA
  ACAGATTTCCTTGTTCCCTTGCTGAGCTCTGTCTTAACTGTGGCTTGGATCTGTTGCTTG
  GTGACGGCCTTCTACTGGTGCCTGCGGAAGCGGCGGAAGCCGGGCAGCCACACACACTCA
  GCCTCTGAGGACAACACCACCAACAACGTGCGGGAGCAGCTGAACCAGATCAAAAACCCC
  ATTGAGAAACATGGGGCCAACACGGTCCCCATCAAGGATTACGAGAACAAGAACTCCAAA
  ATGTCTAAAATAAGGACACACAATTCTGAAGTAGAAGAGGACGACATGGACAAACACCAG
  CAGAAAGCCCGGTTTGCCAAGCAGCCGGCGTATACGCTGGTAGACAGAGAAGAGAAGCCC
  CCCAACGGCACGCCGACAAAACACCCAAACTGGACAAACAAACAGGACAACAGAGACTTG
  GAAAGTGCCCAGAGCTTAAACCGAATGGAGTACATCGTATAGCAGACCGCGGGCACTGCC
  GCCGCTAGGTAGAGTCTGAGGGCTTGTAGTTCTTTAAACTGTCGTGTCATACTCGAGTCT
  GAGGCCGTTGCTGACTTXXXXXXXXXXXXXXXXXXXXGTATGTGTTTAGAATTGAAGGTT
  TTTGATAGCATTGTAAGCGTATGGCTTTATTTTTTTGAACTCTTCTCATTACTTGTTGCC
  TATAAGCCAAAATTAAGGTGTTTGAAAATAGTTTATTTTAAAACAATAGGATGGGCTTCT
  GTGCCCAGAATACTGATGGAATTTTTTTTGTACGACGTCAGATGTTTAAAACACCTTCTA
  TAGCATCACTTAAAACACGTTTTAAGGACTGACTGAGGCAGTTTGAGGATTAGTTTAGAA
  CAGGTTTTTTTGTTTGTTTGTTTTTTGTTTTTCTGCTTTAGACTTGAAAAGAGACAGGCA
  GGTGATCTGCTGCAGAGCAGTAAGGGAACAAGTTGAGCTATGACTTAACATAGCCAAAAT
  GTGAGTGGTTGAATATGATTAAAAATATCAAATTAATTGTGTGAACTTGGAAGCACACCA
  ATCTGACTTTGTAAATTCTGATTTCTTTTCACCATTCGTACATAATACTGAACCACTTGT
  AGATTTGATTTTTTTTTTAATCTACTGCATTTAGGGAGTATTCTAATAAGCTAGTTGAAT
  ACTTGAACCATAAAATGTCCAGTAAGATCACTGTTTAGATTTGCCATAGAGTACACTGCC
  TGCCTTAAGTGAGGAAATCAAAGTGCTATTACGAAGTTCAAGATCAAAAAGGCTTATAAA
  ACAGAGTAATCTTGTTGGTTCACCATTGAGACCGTGAAGATACTTTGTATTGTCCTATTA
  GTGTTATATGAACATACAAATGCATCTTTGATGTGTTGTTCTTGGCAATAAATTTTGAAA
  AGTAATATTTATTAAATTTTTTTGTATGAAAACAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000214 unedited
  NNNNTTTGTTCGAATTTTGTCATACGACTCATATAGGCGGCCCGCGAATCGGCACGAGCT
  TCTCCGAGAGCCGGGCGGGCACGCGTCATTGTGTTACCTGCGGCCGGCCCGCGAGCTAGG
  CTGGTTTTTTTTTTTCTCCCCTCCCTCCCCCCTTTTTCCATGCAGCTGATCTAAAAGGGA
  ATAAAAGGCTGCGCATAATCATAATAATAAAAGAAGGGGAGCGCGAGAGAAGGAAAGAAA
  GCCGGGAGGTGGAAGAGGAGGGGGAGCGTCTCAAAGAAGCGATCAGAATAATAAAAGGAG
  GCCGGGCTCTTTGCCTTCTGGAACGGGCCGCTCTTGAAAGGGCTTTTGAAAAGTGGTGTT
  GTTTTCCAGTCGTGCATGCTCCAATCGGCGGAGTATATTAGAGCCGGGACGCGGCGGCCG
  CAGGGGCAGCGGCGACGGCAGCACCGGCGGCAGCACCAGCGCGAACAGCAGCGGCGGCGT
  CCCGAGTGCCCGCGGCGCGCGGCGCAGCGATGCGTTCCCCACGGACGCGCGGCCGGTCCG
  GGCGCCCCCTAAGCCTCCTGCTCGCCCTGCTCTGTGCCCTGCGAGCCAAGGTGTGTGGGG
  CCTCGGGTCAGTTCGAGTTGGAGATCCTGTCCATGCAGAACGTGAACGGGGAGCTGCAGA
  ACGGGAACTGCTGCGGCGGCGCCCGGAACCCGGGAGACCGCAAGTGCACCCGCGACGAGT
  GCGACACATACTTCAAAGTGTGCCTCAAGGAGTATCAGTCCCGCGTCACGGCCCGGGGGG
  CCCTGCAGCTTTCGCTCAAGGTCCACGCCTGTCATTCGGGGCCAACACCTTCAACCTCAA
  GGCCAGCCGCGGAACGACCGACCCCGCATNGTGCTGCCCTTTAAGTTTCCTCTCGGCCAA
  GCCCTTACCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000214 unedited
  TTTGACGTATTCCCGCCTGACGGTCGAGAATATTACCTTTTTTTTTGGNTNNCATACATT
  TTTAATTTAATAAATATTACTTCTTCATAATTTATTGCCAAGAACAACACATCAAAGATG
  CATTTGTATGTTCATATAACACTAATAGGACAATACAAAGTATCTTCACGGTCTCAATGG
  TGAACCAACAAGATTACTCTGGNATATAAACTCCCGCTGCGCCTGAACTTCGNAATAGCA
  CTTTGATTTCCTCACTTAAGGCAGGCAGTGTACTCTATGGCAAATCTAAACAGGGATCTT
  ACTGGACATTTTATGGGTCAAGTATTCAACTAGCTTATTAGAATACTCCCTAAATGCAGC
  AGATTAAAAAAAAAATCAAATCTACAAGTGGTTCAGCATTATGTACGAATGGTGAAAAGA
  AATCAGAATTTACAAAGTCAGATTGGTGTGCTTCCAAGTTCACACAATTAATTTGATATT
  TTTAATCATATTCAACCACTCACATTTTGGCTATGTTAAGTCATAGCTCAACTTGTTCCC
  TTACTGCTCTGCAGCAGATCACCTGCCTGGCTCTTTTCAAGTCTAAAGCAGAAAAACAAA
  AAACAAACAAACAAAAAAACCCGTTCTAAACTAATCCTCAAACTGCCTCAGTCAGCCCTT
  AAAACGCGTCTTAAGTGATGCTATAGAAAGCGTTTTAAACATCTGACGTCGTACAAAAAA
  AATTCCATCAGTATTTCTGGGCACAGAAGCCCATCCTATTGTTTTAAAATAAACTATTTT
  CAAACACCTTAATTTTGGCTTATAGGCCACAAGGAATGAGAAGAGTTCAAATAAATAAGG
  CATACGCTTACAATGCTTCCAAAACCTTCCCTTTCTACCCTCCCTTTAATTAAAAGGATG
  AGCTTAAAGCTCTTGATATTACGTATAAACAATTTCCCCGGTTATAAAAAGATTATTTCA
  TACTTACAAGATCTATTTGAATTTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000214
• Insert Size: 5800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000214.1, NP_000205.1
• RefSeq Size: 5896 bp
• RefSeq ORF: 3657 bp
• Locus ID: 182
• UniProt ID: P78504
• Cytogenetics: 20p12.2
• Domains: VWC, DSL, EGF_CA, EGF, EGF, VWC_out
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Notch signaling pathway
• Gene Summary: The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter is involved in signaling processes. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq, Nov 2019]