Factor I (CFI) (NM_000204) Human Untagged Clone

CAT#: SC120051

CFI (untagged)-Human complement factor I (CFI)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Factor I
• Synonyms: AHUS3; ARMD13; C3b-INA; C3BINA; FI; IF; KAF
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120051 sequence for NM_000204 edited (data generated by NextGen Sequencing)
  ATGAAGCTTCTTCATGTTTTCCTGTTATTTCTGTGCTTCCACTTAAGGTTTTGCAAGGTC
  ACTTATACATCTCAAGAGGATCTGGTGGAGAAAAAGTGCTTAGCAAAAAAATATACTCAC
  CTCTCCTGCGATAAAGTCTTCTGCCAGCCATGGCAGAGATGCATTGAGGGCACCTGTGTT
  TGTAAACTACCGTATCAGTGCCCAAAGAATGGCACTGCAGTGTGTGCAACTAACAGGAGA
  AGCTTCCCAACATACTGTCAACAAAAGAGTTTGGAATGTCTTCATCCAGGGACAAAGTTT
  TTAAATAACGGAACATGCACAGCCGAAGGAAAGTTTAGTGTTTCCTTGAAGCATGGAAAT
  ACAGATTCAGAGGGAATAGTTGAAGTAAAACTTGTGGACCAAGATAAGACAATGTTCATA
  TGCAAAAGCAGCTGGAGCATGAGGGAAGCCAACGTGGCCTGCCTTGACCTTGGGTTTCAA
  CAAGGTGCTGATACTCAAAGAAGGTTTAAGTTGTCTGATCTCTCTATAAATTCCACTGAA
  TGTCTACATGTGCATTGCCGAGGATTAGAGACCAGTTTGGCTGAATGTACTTTTACTAAG
  AGAAGAACTATGGGTTACCAGGATTTCGCTGATGTGGTTTGTTATACACAGAAAGCAGAT
  TCTCCAATGGATGACTTCTTTCAGTGTGTGAATGGGAAATACATTTCTCAGATGAAAGCC
  TGTGATGGTATCAATGATTGTGGAGACCAAAGTGATGAACTGTGTTGTAAAGCATGCCAA
  GGCAAAGGCTTCCATTGCAAATCAGGTGTTTGCATTCCAAGCCAGTATCAATGCAATGGT
  GAGGTGGACTGCATTACAGGGGAAGATGAAGTTGGCTGTGCAGGCTTTGCATCTGTGGCT
  CAAGAAGAAACAGAAATTTTGACTGCTGACATGGATGCAGAAAGAAGACGGATAAAATCA
  TTATTACCTAAACTATCTTGTGGAGTTAAAAACAGAATGCACATTCGAAGGAAACGAATT
  GTGGGAGGAAAGCGAGCACAACTGGGAGACCTCCCATGGCAGGTGGCAATTAAGGATGCC
  AGTGGAATCACCTGTGGGGGAATTTATATTGGTGGCTGTTGGATTCTGACTGCTGCACAT
  TGTCTCAGAGCCAGTAAAACTCATCGTTACCAAATATGGACAACAGTAGTAGACTGGATA
  CACCCCGACCTTAAACGTATAGTAATTGAATACGTGGATAGAATTATTTTCCATGAAAAC
  TACAATGCAGGCACTTACCAAAATGACATCGCTTTGATTGAAATGAAAAAAGACGGAAAC
  AAAAAAGATTGTGAGCTGCCTCGTTCCATCCCTGCCTGTGTCCCCTGGTCTCCTTACCTA
  TTCCAACCTAATGATACATGCATCGTTTCTGGCTGGGGACGAGAAAAAGATAACGAAAGA
  GTCTTTTCACTTCAGTGGGGTGAAGTTAAACTAATAAGCAACTGCTCTAAGTTTTACGGA
  AATCGTTTCTATGAAAAAGAAATGGAATGTGCAGGTACATATGATGGTTCCATCGATGCC
  TGTAAAGGGGACTCTGGAGGCCCCTTAGTCTGTATGGATGCCAACAATGTGACTTATGTC
  TGGGGTGTTGTGAGTTGGGGGGAAAACTGTGGAAAACCAGAGTTCCCAGGTGTTTACACC
  AAAGTGGCCAATTATTTTGACTGGATTAGCTACCATGTAGGAAGGCCTTTTATTTCTCAG
  TACAATGTATAA
  
  Clone variation with respect to NM_000204.3
  804 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000204 unedited
  NGGGGTCACCATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACCAGNAGAAAA
  ANACCCCGACACCTCCAACATGAACTTCTTCATGTTTTCCTGTTATTTCTGTGCTTCCAC
  TTAAGGTTTTGCAAGGTCACTTATACATCTCAAGAGGATCTGGTGGAGAAAAAGTGCTTA
  GCAAAAAAATATACTCACCTCTCCTGCGATAAAGTCTTCTGCCAGCCATGGCAGAGATGC
  ATTGAGGGCACCTGTGTTTGTAAACTACCGTATCAGTGCCCAAAGAATGGCACTGCAGTG
  TGTGCAACTAACAGGAGAAGCTTCCCAACATACTGTCAACAAAAGAGTTTGGAATGTCTT
  CATCCAGGGACAAAGTTTTTAAATAACGGAACATGCACAGCCGAAGGAAAGTTTAGTGTT
  TCCTTGAAGCATGGAAATACAGATTCAGAGGGAATAGTTGAAGTAAAACTTGTGGACCAA
  GATAAGACAATGTTCATATGCAAAAGCAGCTGGAGCATGAGGGAAGCCAACGTGGCCTGC
  CTTGACCTTGGGTTTCAACAAGGTGCTGATACTCAAAGAAGGTTTAAGTTGTCTGATCTC
  TCTATAAATTCCACTGAATGTCTACATGTGCATTGCCGAGGATTAGAGACCAGTTTGGCT
  GAATGTACTTTTACTAAGAGAAGAACTATGGGTTACCAGGATTTCGCTGATGTGGTTTTG
  TTATACACAGAAAGCAGATTCTCCAATGGATGACTTCTTTCAGTGTGTGAATGGGAAATA
  CATTTCTCAGATGAAAGCCTGTGATGTTATCAATGATTGTGGAGACCAAATGATGAACTG
  TGTTGTAAACATGCCAAGGCAAAGCTTCCATTGCAAATAGGGGTTTGCATTCCAGCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000204 unedited
  NTTTAGCTTGNACCCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTT
  TATGCTTCACCAAANATATTTATTTGAGAATTATACAACAAAATTCCAATATGGCATAAA
  CTCTGTGGAGACCTTTAAAAATATCCAATGAGATTTGCTTCATTTTTCCCCCCTAGAGAA
  TTATTAATTATACCGTTTTATTTCCATTAAATGGAACTCTTGAGAGAAAAAGAATAGAAT
  GAAGAGAGAGATCACAATTTTATACATTGTACTGAGAAATAAAAGGCCTTCCTACATGGT
  AGCTAATCCAGTCAAAATAATTGGCCACTTTGGTGTAAACACCTGGGAACTCTGGTTTTC
  CACAGTTTTCCCCCCAACTCACAACACCCCAGACATAAGTCACATTGTTGGCATCCATAC
  AGACTAAGGGGCCTCCAGAGTCCCCTTTACAGGCATCGATGGAACCATCATATGTACCTG
  CACATTCCATTTCTTTTTCATAGAAACGATTTCCGTAAAACTTAGAGCAGTTGCTTATTA
  GTTTAACTTCACCCCACTGAAGTGAAAAGACTCTTTCGTTATCTTTTTCTCGTCCCCAGC
  CAGAAACGATGCATGTATCATTAGGTTGGAATAGGTAAGGAGACCAGGGGACACAGGCAG
  GGATGGAACGAGGCAGCTCACAATCTTTTTTGTTTCCGTCTTTTTTCATTTCAATCAAAG
  CGATGTCATTTTGGTAAGTGCCTGCATTGTAGTTTTCATGGAAAATAATTCTATCCACGT
  ATTCAATTACTATACGTTTAAGGTCGGGGTGTATCCAGTCTACTACTGTTGTCCATATTT
  GGTACGATGAGTTTTACTGGCTCTGAGNACATGTGCAGCAGTCAGAATCCACAGCCACCC
  ATATAAATCCCCACAGGTGATNCACTGGCATNCTTAATGCCCCTGCATGGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000204
• Insert Size: 2260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000204.1, NP_000195.1
• RefSeq Size: 1963 bp
• RefSeq ORF: 1752 bp
• Locus ID: 3426
• UniProt ID: P05156
• Cytogenetics: 4q25
• Domains: SR, Tryp_SPc, ldl_recept_a, FIMAC
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uremic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immune deposits and age-related macular degeneration are other conditions associated with mutations of this gene. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (2) encodes isoform 2.