MSH2 (NM_000251) Human Untagged Clone

CAT#: SC119995

MSH2 (untagged)-Human mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) (MSH2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MSH2
• Synonyms: COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2; MMRCS2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119995 sequence for NM_000251 edited (data generated by NextGen Sequencing)
  ATGGCGGTGCAGCCGAAGGAGACGCTGCAGTTGGAGAGCGCGGCCGAGGTCGGCTTCGTG
  CGCTTCTTTCAGGGCATGCCGGAGAAGCCGACCACCACAGTGCGCCTTTTCGACCGGGGC
  GACTTCTATACGGCGCACGGCGAGGACGCGCTGCTGGCCGCCCGGGAGGTGTTCAAGACC
  CAGGGGGTGATCAAGTACATGGGGCCGGCAGGAGCAAAGAATCTGCAGAGTGTTGTGCTT
  AGTAAAATGAATTTTGAATCTTTTGTAAAAGATCTTCTTCTGGTTCGTCAGTATAGAGTT
  GAAGTTTATAAGAATAGAGCTGGAAATAAGGCATCCAAGGAGAATGATTGGTATTTGGCA
  TATAAGGCTTCTCCTGGCAATCTCTCTCAGTTTGAAGACATTCTCTTTGGTAACAATGAT
  ATGTCAGCTTCCATTGGTGTTGTGGGTGTTAAAATGTCCGCAGTTGATGGCCAGAGACAG
  GTTGGAGTTGGGTATGTGGATTCCATACAGAGGAAACTAGGACTGTGTGAATTCCCTGAT
  AATGATCAGTTCTCCAATCTTGAGGCTCTCCTCATCCAGATTGGACCAAAGGAATGTGTT
  TTACCCGGAGGAGAGACTGCTGGAGACATGGGGAAACTGAGACAGATAATTCAAAGAGGA
  GGAATTCTGATCACAGAAAGAAAAAAAGCTGACTTTTCCACAAAAGACATTTATCAGGAC
  CTCAACCGGTTGTTGAAAGGCAAAAAGGGAGAGCAGATGAATAGTGCTGTATTGCCAGAA
  ATGGAGAATCAGGTTGCAGTTTCATCACTGTCTGCGGTAATCAAGTTTTTAGAACTCTTA
  TCAGATGATTCCAACTTTGGACAGTTTGAACTGACTACTTTTGACTTCAGCCAGTATATG
  AAATTGGATATTGCAGCAGTCAGAGCCCTTAACCTTTTTCAGGGTTCTGTTGAAGATACC
  ACTGGCTCTCAGTCTCTGGCTGCCTTGCTGAATAAGTGTAAAACCCCTCAAGGACAAAGA
  CTTGTTAACCAGTGGATTAAGCAGCCTCTCATGGATAAGAACAGAATAGAGGAGAGATTG
  AATTTAGTGGAAGCTTTTGTAGAAGATGCAGAATTGAGGCAGACTTTACAAGAAGATTTA
  CTTCGTCGATTCCCAGATCTTAACCGACTTGCCAAGAAGTTTCAAAGACAAGCAGCAAAC
  TTACAAGATTGTTACCGACTCTATCAGGGTATAAATCAACTACCTAATGTTATACAGGCT
  CTGGAAAAACATGAAGGAAAACACCAGAAATTATTGTTGGCAGTTTTTGTGACTCCTCTT
  ACTGATCTTCGTTCTGACTTCTCCAAGTTTCAGGAAATGATAGAANCAACTTTAGATATG
  GATCAGGTGGAAAACCATGAATTCCTTGTAAAACCTTCATTTGATCCTAATCTCAGTGAA
  TTAAGAGAAATAATGAATGACTTGGAAAAGAAGATGCAGTCAACATTAATAAGTGCAGCC
  AGAGATCTTGGCTTGGACCCTGGCAAACAGATTAAACTGGATTCCAGTGCACAGTTTGGA
  TATTACTTTCGTGTAACCTGTAAGGAAGAAAAAGTCCTTCGTAACAATAAAAACTTTAGT
  ACTGTAGATATCCAGAAGAATGGTGTTAAATTTACCAACAGCAAATTGACTTCTTTAAAT
  GAAGAGTATACCAAAAATAAAACAGAATATGAAGAAGCCCAGGATGCCATTGTTAAAGAA
  ATTGTCAATATTTCTTCAGGCTATGTAGAACCAATGCAGACACTCAATGATGTGTTAGCT
  CAGCTAGATGCTGTTGTCAGCTTTGCTCACGTGTCAAATGGAGCACCTGTTCCATATGTA
  CGACCAGCCATTTTGGAGAAAGGACAAGGAAGAATTATATTAAAAGCATCCAGGCATGCT
  TGTGTTGAAGTTCAAGATGAAATTGCATTTATTCCTAATGACGTATACTTTGAAAAAGAT
  AAACAGATGTTCCACATCATTACTGGCCCCAATATGGGAGGTAAATCAACATATATTCGA
  CAAACTGGGGTGATAGTACTCATGGCCCAAATTGGGTGTTTTGTGCCATGTGAGTCAGCA
  GAAGTGTCCATTGTGGACTGCATCTTAGCCCGAGTAGGGGCTGGTGACAGTCAATTGAAA
  GGAGTCTCCACGTTCATGGCTGAAATGTTGGAAACTGCTTCTATCCTCAGGTCTGCAACC
  AAAGATTCATTAATAATCATAGATGAATTGGGAAGAGGAACTTCTACCTACGATGGATTT
  GGGTTAGCATGGGCTATATCAGAATACATTGCAACAAAGATTGGTGCTTTTTGCATGTTT
  GCAACCCATTTTCATGAACTTACTGCCTTGGCCAATCAGATACCAACTGTTAATAATCTA
  CATGTCACAGCACTCACCACTGAAGAGACCTTAACTATGCTTTATCAGGTGAAGAAAGGT
  GTCTGTGATCAAAGTTTTGGGATTCATGTTGCAGAGCTTGCTAATTTCCCTAAGCATGTA
  ATAGAGTGTGCTAAACAGAAAGCCCTGGAACTTGAGGAGTTTCAGTATATTGGAGAATCG
  CAAGGATATGATATCATGGAACCAGCAGCAAAGAAGTGCTATCTGGAAAGAGAGCAAGGT
  GAAAAAATTATTCAGGAGTTCCTGTCCAAGGTGAAACAAATGCCCTTTACTGAAATGTCA
  GAAGAAAACATCACAATAAAGTTAAAACAGCTAAAAGCTGAAGTAATAGCAAAGAATAAT
  AGCTTTGTAAATGAAATCATTTCACGAATAAAAGTTACTACGTGA
  
  Clone variation with respect to NM_000251.1
  1366 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000251 unedited
  TGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGAGGAGGTTTCGACATGGC
  GGTGCAGCCGAAGGAGACGCTGCAGTTGGAGAGCGCGGCCGAGGTCGGCTTCGTGCGCTT
  CTTTCAGGGCATGCCGGAGAAGCCGACCACCACAGTGCGCCTTTTCGACCGGGGCGACTT
  CTATACGGCGCACGGCGAGGACGCGCTGCTGGCCGCCCGGGAGGTGTTCAAGACCCAGGG
  GGTGATCAAGTACATGGGGCCGGCAGGAGCAAAGAATCTGCAGAGTGTTGTGCTTAGTAA
  AATGAATTTTGAATCTTTTGTAAAAGATCTTCTTCTGGTTCGTCAGTATAGAGTTGAAGT
  TTATAAGAATAGAGCTGGAAATAAGGCATCCAAGGAGAATGATTGGTATTTGGCATATAA
  GGCTTCTCCTGGCAATCTCTCTCAGTTTGAAGACATTCTCTTTGGTAACAATGATATGTC
  AGCTTCCATTGGTGTTGTGGGTGTTAAAATGTCCGCAGTTGATGGCCAGAGACAGGTTGG
  AGTTGGGTATGTGGATTCCATACAGAGGAAACTAGGACTGTGTGAATTCCCTGATAATGA
  TCAGTTCTCCAATCTTGAGGCTCTCCTCATCCAGATTGGACCAAGGAATGTGTTTTACCC
  GGAGGAGAGACTGCTGGAGACATGGNGAAACTGAGACAGATAATTAAAGAGGAGGAATTC
  TGATCACAGAAGAANAAAGCTGACTNTTCACAAAGACATTATCAGACCTCACCCGNNTGT
  GAAGGCAAAGGGAGAGCAATGATAGTGCTNGATGCCAGATGGAGAATCAGGTGCAGTTCA
  NACTGGCTGCGGNATCAGTTTTAGACTCTATAGATGATCCACTTGGGACGTTGAACTGCT
  ACTTTGACTCGCCGNTATGATTGGATGGCGCNTCAGCCTAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000251 unedited
  CTCTGCATTTTTTTATAAAATTCAGCACATCACTTATTATTGCCTATGTCAATTGCAAAC
  AGTCCTCAGTTACAGCTCTCATTTTTCAAAATAAAAATCTTTGAAAATGTCCTCAAAGTA
  AAATATTACTAAATATCTTATTAAGTTGATAGCCCATGGGCACTGACAGTTAACACTATG
  GAAAAAGGGTTAATATAAAACAATATAAAACTATTACAGACAATAGCTTATCAATATTAC
  CTTCATTCCATTACTGGGATTTTTCACGTAGTAACTTTTATTCGTGAAATGATTTCATTT
  ACAAAGCTATTATTCTTTGCTATTACTTCAGCTTTTAGCTGTTTTAACTTTATTGTGATG
  TTTTCTTCTGACATTTCAGTAAAGGGCATTTGTTTCACCTTGGACAGGAACTCCTGAATA
  ATTTTTTCACCTTGCTCTCTTTCCAGATAGCACTTCTTTGCTGCTGGTTCCATGATATCA
  TATCCTTGCGATTCTCCAATATACTGAAACTCCTCAAGTTCCAGGGCTTTCTGTTTAGCA
  CACTCTATTACATGCTTAGGGAAATTAGCAAGCTCTGCAACATGAATCCCAAAACTTTGA
  TCACAGACACCTTTCTTCACCTGATAAAGCATAGTTAAGGTCTCTTCAGTGGTGAGTGCT
  GTGACATGTAGATTATTAACAGTTGGTATCTGATTGGCCAAGGCAGTAAGTTCATGACAA
  TGGGTTGCAAACATGCANAAAGCACCAAATCTTGTTGCAATGTATTCTGATATAGCCCAT
  GCTAACCAAATNCATCGTAGGTAGAAAGTTCTCTTCCAANTCATCTATGATTATTAATGA
  AATCTTTGGTGCAGACCTGAGGATAGAAGCAGTTTCCAACATTTCACCATGAACCGTGAG
  ACTNCTTTCAATNGACTGTTCACAGCCCCTACTCGGGCTAAGATGCAGTCACAATGGACA
  CCTCTGCTGACTAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000251
• Insert Size: 3660 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000251.1, NP_000242.1
• RefSeq Size: 3145 bp
• RefSeq ORF: 2805 bp
• Locus ID: 4436
• UniProt ID: P43246
• Cytogenetics: 2p21-p16.3
• Domains: MutS_V, MutS_I, MutS_III, MutS_II, MutS_IV
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Colorectal cancer, Mismatch repair, Pathways in cancer
• Gene Summary: This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).