Parathyroid Hormone Receptor 1 (PTH1R) (NM_000316) Human Untagged Clone

CAT#: SC119966

PTH1R (untagged)-Human parathyroid hormone 1 receptor (PTH1R), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Parathyroid Hormone Receptor 1
• Synonyms: EKNS; PFE; PTHR; PTHR1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000316, RT-PCR generated
  ATGGGGACCGCCCGGATCGCACCCGGCCTGGCGCTCCTGCTCTGCTGCCCCGTGCTCAGC
  TCCGCGTACGCGCTGGTGGATGCAGATGACGTCATGACTAAAGAGGAACAGATCTTCCTG
  CTGCACCGTGCTCAGGCCCAGTGCGAAAAACGGCTCAAGGAGGTCCTGCAGAGGCCAGCC
  AGCATAATGGAATCAGACAAGGGATGGACATCTGCGTCCACATCAGGGAAGCCCAGGAAA
  GATAAGGCATCTGGGAAGCTCTACCCTGAGTCTGAGGAGGACAAGGAGGCACCCACTGGC
  AGCAGGTACCGAGGGCGCCCCTGTCTGCCGGAATGGGACCACATCCTGTGCTGGCCGCTG
  GGGGCACCAGGTGAGGTGGTGGCTGTGCCCTGTCCGGACTACATTTATGACTTCAATCAC
  AAAGGCCATGCCTACCGACGCTGTGACCGCAATGGCAGCTGGGAGCTGGTGCCTGGGCAC
  AACAGGACGTGGGCCAACTACAGCGAGTGTGTCAAATTTCTCACCAATGAGACTCGTGAA
  CGGGAGGTGTTTGACCGCCTGGGCATGATTTACACCGTGGGCTACTCCGTGTCCCTGGCG
  TCCCTCACCGTAGCTGTGCTCATCCTGGCCTACTTTAGGCGGCTGCACTGCACGCGCAAC
  TACATCCACATGCACCTGTTCCTGTCCTTCATGCTGCGCGCCGTGAGCATCTTCGTCAAG
  GACGCTGTGCTCTACTCTGGCGCCACGCTTGATGAGGCTGAGCGCCTCACCGAGGAGGAG
  CTGCGCGCCATCGCCCAGGCGCCCCCGCCGCCTGCCACCGCCGCTGCCGGCTACGCGGGC
  TGCAGGGTGGCTGTGACCTTCTTCCTTTACTTCCTGGCCACCAACTACTACTGGATTCTG
  GTGGAGGGGCTGTACCTGCACAGCCTCATCTTCATGGCCTTCTTCTCAGAGAAGAAGTAC
  CTGTGGGGCTTCACAGTCTTCGGCTGGGGTCTGCCCGCTGTCTTCGTGGCTGTGTGGGTC
  AGTGTCAGAGCTACCCTGGCCAACACCGGGTGCTGGGACTTGAGCTCCGGGAACAAAAAG
  TGGATCATCCAGGTGCCCATCCTGGCCTCCATTGTGCTCAACTTCATCCTCTTCATCAAT
  ATCGTCCGGGTGCTCGCCACCAAGCTGCGGGAGACCAACGCCGGCCGGTGTGACACACGG
  CAGCAGTACCGGAAGCTGCTCAAATCCACGCTGGTGCTCATGCCCCTCTTTGGCGTCCAC
  TACATTGTCTTCATGGCCACACCATACACCGAGGTCTCAGGGACGCTCTGGCAAGTCCAG
  ATGCACTATGAGATGCTCTTCAACTCCTTCCAGGGATTTTTTGTCGCAATCATATACTGT
  TTCTGCAACGGCGAGGTACAAGCTGAGATCAAGAAATCTTGGAGCCGCTGGACACTGGCA
  CTGGACTTCAAGCGAAAGGCACGCAGCGGGAGCAGCAGCTATAGCTACGGCCCCATGGTG
  TCCCACACAAGTGTGACCAATGTCGGCCCCCGTGTGGGACTCGGCCTGCCCCTCAGCCCC
  CGCCTACTGCCCACTGCCACCACCAACGGCCACCCTCAGCTGCCTGGCCATGCCAAGCCA
  GGGACCCCAGCCCTGGAGACCCTCGAGACCACACCACCTGCCATGGCTGCTCCCAAGGAC
  GATGGGTTCCTCAACGGCTCCTGCTCAGGCCTGGACGAGGAGGCCTCTGGGCCTGAGCGG
  CCACCTGCCCTGCTACAGGAAGAGTGGGAGACAGTCATGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000316 unedited
  GGGGAATGTCGGGATTTGTAAACGATTACTATAGGCGGCCGCCAGTCGTGATGGAATCTG
  CAGAATCGGCTTGGTGGCGATGGGGACCGCCCGGGTCGCACCCGGCCTGGCGCTCCTGCT
  CTGCTGCCCCGTGCTCAGCTCCGCGTACGCGCTGGTGGATGCAGATGACGTCATGACTAA
  AGAGGAACAGATCTTCCTGCTGCACCGTGCTCAGGCCCAGTGCGAAAAACGGCTCAAGGA
  GGTCCTGCAGAGGCCAGCCAGCATAATGGAATCAGACAAGGGATGGACATCTGCGTCCAC
  ATCAGGGAAGCCCAGGAAAGATAAGGCATCTGGGAAGCTCTACCCTGAGTCTGAGGAGGA
  CAAGGAGGCACCCACTGGCAGCAGGTACCGAGGGCGCCCCTGTCTGCCGGAATGGGACCA
  CATCCTGTGCTGGCCGCTGGGGGCACCAGGTGAGGTGGTGGCTGTGCCCTGTCCGGACTA
  CATTTATGACTTCAATCACAAAGGCCATGCCTACCGACGCTGTGACCGCAATGGCAGCTG
  GGAGCTGGTGCCTGGGCACAACAGGACGTGGGCCAACTACAGCGAGTGTGTCAAATTTCT
  CACCAATGAGACTCGTGAACGGGAGGTGTTTGACCGCCTGGGCATGATTTACACCGTGGG
  CTACTCCGTGTCCCTGGCGTCCCTCACCGTAGCTGTGCTCATCCTGGCCTACTTTAGGCG
  GCTGCACTGCACGCGCAACTACATCCACATGCACCTGTTCCTGTCCTTCATGCTGCGCGC
  CGTGAGCATCTTCGTCAAGGACGCTGTGCTCTACTCTGGCGCCACGCTGATGAGGCTGAG
  CGCCTCACCGAGGAGAGCTGCGCGCCATCGTCCAGGCGCCCCCGACAGCTGCCACCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000316 unedited
  CACTGGAGGCAGGGGCTCACAGAGGCACTGCCACTCCGGGTATCTGTTCAGGAAAACAGC
  TATGACCGCGGCCGCAATCTATAGTCGACAAGCTTGATATCGGTACCGAGCTCGGATCCA
  CTAGTAACGGCCGCCAGTGTGCTGGAATTCGGCTTCGCCTGGTCACATGACTGTCTCCCA
  CTCTTCCTGTATCAGGGCAGGTGGCCGCTCAGGCCCAGAGGCCTCCTCGTCCAGGCCTGA
  GCAGGAGCCGTTGAGGAACCCATCGTCCTTGGGAGCAGCCATGGCAGGTGGTGTGGTCTC
  GAGGGTCTCCAGGGCTGGGGTCCCTGGCTTGGCATGGCCAGGCAGCTGAAGGTGGCCGTT
  GGTGGTGGCAGTGGGCAGTAGGCGGGGGCTGAGGGGCAGGCCGAGTCCCACACGGGGGCC
  GACATTGGTCACACTTGTGTGGGACACCATGGGGCCGTAGCTATAGCTGCTGCTCCCGCT
  GCGTGCCTTTCGCTTGAAGTCCAGTGCCAGTGTCCAGCGGCTCCAAGATTTCTTGATCTC
  AGCTTGTACCTCGCCGTTGCAGAAACAGTATATGATTGCGACAAAAAATCCCTGGAAAGA
  GTTGAAGAGCATCTCATAGTGCATCTGGACTTGCCAGAGCGTCCCTGAGACCTCGGTGTA
  TGGTGTGGCCATGAAGACAATGTAGTGGACGCCAAAGAGGGGCATGAGCACCAGCGTGGA
  TTTGAGCAGCTTCCGGTACTGCTGCCGTGTGTCACACCGGCCGGCGTTGGTCTCCCGCAG
  CTTGGTGGCGAGCACCCGNACGATTTTGATGAAGAAGATGAAGTTGAGCACAATGGAAGC
  CAGGT
  
• Restriction Sites: Please inquire
• ACCN: NM_000316
• Insert Size: 2090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000316.2, NP_000307.1
• RefSeq Size: 2144 bp
• RefSeq ORF: 1782 bp
• Locus ID: 5745
• UniProt ID: Q03431
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: The protein encoded by this gene is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in this receptor are known to be the cause of Jansen's metaphyseal chondrodysplasia (JMC), chondrodysplasia Blomstrand type (BOCD), as well as enchodromatosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.