Protein S (PROS1) (NM_000313) Human Untagged Clone

CAT#: SC119963

PROS1 (untagged)-Human protein S (alpha) (PROS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Protein S
• Synonyms: PROS; PS21; PS22; PS23; PS24; PS25; PSA; THPH5; THPH6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119963 sequence for NM_000313 edited (data generated by NextGen Sequencing)
  ATGAGGGTCCTGGGTGGGCGCTGCGGGGCGCTGCTGGCGTGTCTCCTCCTAGTGCTTCCC
  GTCTCAGAGGCAAACTTTTTGTCAAAGCAACAGGCTTCACAAGTCCTGGTTAGGAAGCGT
  CGTGCAAATTCTTTACTTGAAGAAACCAAACAGGGTAATCTTGAAAGAGAATGCATCGAA
  GAACTGTGCAATAAAGAAGAAGCCAGGGAGGTCTTTGAAAATGACCCGGAAACGGATTAT
  TTTTATCCAAAATACTTAGTTTGTCTTCGCTCTTTTCAAACTGGGTTATTCACTGCTGCA
  CGTCAGTCAACTAATGCTTATCCTGACCTAAGAAGCTGTGTCAATGCCATTCCAGACCAG
  TGTAGTCCTCTGCCATGCAATGAAGATGGATATATGAGCTGCAAAGATGGAAAAGCTTCT
  TTTACTTGCACTTGTAAACCAGGTTGGCAAGGAGAAAAGTGTGAATTTGACATAAATGAA
  TGCAAAGATCCCTCAAATATAAATGGAGGTTGCAGTCAAATTTGTGATAATACACCTGGA
  AGTTACCACTGTTCCTGTAAAAATGGTTTTGTTATGCTTTCAAATAAGAAAGATTGTAAA
  GATGTGGATGAATGCTCTTTGAAGCCAAGCATTTGTGGCACAGCTGTGTGCAAGAACATC
  CCAGGAGATTTTGAATGTGAATGCCCCGAAGGCTACAGATATAATCTCAAATCAAAGTCT
  TGTGAAGATATAGATGAATGCTCTGAGAACATGTGTGCTCAGCTTTGTGTCAATTACCCT
  GGAGGTTACACTTGCTATTGTGATGGGAAGAAAGGATTCAAACTTGCCCAAGATCAGAAG
  AGTTGTGAGGTTGTTTCAGTGTGCCTTCCCTTGAACCTTGACACAAAGTATGAATTACTT
  TACTTGGCGGAGCAGTTTGCAGGGGTTGTTTTATATTTAAAATTTCGTTTGCCAGAAATC
  AGCAGATTTTCAGCAGAATTTGATTTCCGGACATATGATTCAGAAGGCGTGATACTGTAC
  GCAGAATCTATCGATCACTCAGCGTGGCTCCTGATTGCACTTCGTGGTGGAAAGATTGAA
  GTTCAGCTTAAGAATGAACATACATCCAAAATCACAACTGGAGGTGATGTTATTAATAAT
  GGTCTATGGAATATGGTGTCTGTGGAAGAATTAGAACATAGTATTAGCATTAAAATAGCT
  AAAGAAGCTGTGATGGATATAAATAAACCTGGACCCCTTTTTAAGCCGGAAAATGGATTG
  CTGGAAACCAAAGTATACTTTGCAGGATTCCCTCGGAAAGTGGAAAGTGAACTCATTAAA
  CCGATTAACCCTCGTCTAGATGGATGTATACGAAGCTGGAATTTGATGAAGCAAGGAGCT
  TCTGGAATAAAGGAAATTATTCAAGAAAAACAAAATAAGCATTGCCTGGTTACTGTGGAG
  AAGGGCTCCTACTATCCTGGTTCTGGAATTGCTCAATTTCACATAGATTATAATAATGTA
  TCCAGTGCTGAGGGTTGGCATGTAAATGTGACCTTGAATATTCGTCCATCCACGGGCACT
  GGTGTTATGCTTGCCTTGGTTTCTGGTAACAACACAGTGCCCTTTGCTGTGTCCTTGGTG
  GACTCCACCTCTGAAAAATCACAGGATATTCTGTTATCTGTTGAAAATACTGTAATATAT
  CGGATACAGGCCCTAAGTCTATGTTCCGATCAACAATCTCATCTGGAATTTAGAGTCAAC
  AGAAACAATCTGGAGTTGTCGACACCACTTAAAATAGAAACCATCTCCCATGAAGACCTT
  CAAAGACAACTTGCCGTCTTGGACAAAGCAATGAAAGCAAAAGTGGCCACATACCTGGGT
  GGCCTTCCAGATGTTCCATTCAGTGCCACACCAGTGAATGCCTTTTATAATGGCTGCATG
  GAAGTGAATATTAATGGTGTACAGTTGGATCTGGATGAAGCCATTTCTAAACATAATGAT
  ATTAGAGCTCACTCATGTCCGTCAGTTTGGAAAAAGACAAAGAATTCTTAA
  
  Clone variation with respect to NM_000313.3
  2001 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000313 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGGTCGCTGGCGCCG
  CCGCGCAGCACGGCTCAGACCGAGGCGCACAGGCTCGCAGCTCCGCGGCGCCTAGCGCTC
  CGGTCCCCGCCGCGACGCGCCACCGTCCCTGCCGGCGCCTCCGCGCGCTTCGAAATGAGG
  GTCCTGGGTGGGCGCTGCGGGGCGCTGCTGGCGTGTCTCCTCCTAGTGCTTCCCGTCTCA
  GAGGCAAACTTTTTGTCAAAGCAACAGGCTTCACAAGTCCTGGTTAGGAAGCGTCGTGCA
  AATTCTTTACTTGAAGAAACCAAACAGGGTAATCTTGAAAGAGAATGCATCGAAGAACTG
  TGCAATAAAGAAGAAGCCAGGGAGGTCTTTGAAAATGACCCGGAAACGGATTATTTTTAT
  CCAAAATACTTAGTTTGTCTTCGCTCTTTTCAAACTGGGTTATTCACTGCTGCACGTCAG
  TCAACTAATGCTTATCCTGACCTAAGAAGCTGTGTCAATGCCATTCCAGACCAGTGTAGT
  CCTCTGCCATGCAATGAAGATGGATATATGAGCTGCAAAGATGGAAAAGCTTCTTTTACT
  TGCACTTGTAAACCAGGTTGGCAAGGAGAAAAGTGTGAATTTGACATAAATGAATGCAAA
  GATCCCTCAAATATAAATGGAGGTTGCAGTCAAATTTGTGATAATACACCTGGAAGTTAC
  CACTGTTCCCTGTAAAATGGTTTTGTTATGCTTTCCAATAAGAAAGATTGTAAAGATGTG
  GATGAATGCTCTTTGAAGCCAAGCATTTGTGGCACAGCTGTGTGCAAGACATCCCCAGAG
  ATTTTGATGTGAATGCCCCGAAGCTACAGAATNATCTCAAACANAGTCTTGTGAAGAATA
  GATGATGCTCTGAGACATGTGTGCTCAGCTTTGGTCACTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000313 unedited
  GGGGAANNNGGGGCTACTGTGNACCGCGGNCCGCTATTTNANGATCGAGTTTTTTTTTTT
  TTTTTTTTAAATTCATATTTAATAGACCACCATCTCTTCTGCCTTCATCAGGAAAAAAAC
  AAAAACATAAACAAAATAGTATCTGCCTATGATTAATAGTATTTAATTACACGCACTTTT
  GTTTGAGTTTACTTCCTTGCTTTCTGAAAAAAACATAGGTATTTAGACACTAGTTCATGA
  TGATAAAATTAAAATTTAGTTTTACAAACAAAAATTGAAACTGTCATTTGTAGGAAAAAA
  ATTCAAATTTAAAATTGTTATTTTTCACTATTCTTAGATAGCAAGAGAAGTAAGAATTTC
  TTTACTGTGATTTATATCACAACAGAATTTTTTTTCCTTGACAAAGGACCTTTTTAAAAA
  TCCCAGAAAAGGACCACAAAATAATCAAAGACTGCACATTGTAAATAAAACCCTTCAGCT
  GGTATTGAAACATAAGTATATTTACACACAGAGAAAAGGTATTATAAGCAGAGAAAAGAT
  GCCTTAAAAATCTTTGTCTTTTTCCAACTGACGACATGAGTGAGCTCTATTATCATATGT
  TTAAAATGGGTTTATCAGATCCAACTGTACACCATAAATTCACTTCCATGCCACCCTTAT
  AAAGGATTCACTGGGGTGGACTTGATGGACATCTGGAAGGCCCCCCAGTTTGGGGCCCCT
  TTTGTTTTATTGGTTGGCCAAAAACGGAAATGGGTTTTAAGGTCTTATGGGAAAGGGGTT
  CTTTTTTAAGGGGGGCCAAACCCCCAAATGTTCTGGTGGCCTTAAATTCCAAAAAAAATG
  GTGGTCGAAAAATAACCTAGGGCCGGTTTCCAATTTTTAAAGTTTTTTCAACAAAAAAAA
  AATCCTGGATTTTTAAAGGGGGGGTCCCCAAGGACCAACAAGGGCCCTGGTTTTCCCCAA
  ACCAGGGGGGTTAAACACGGGCGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000313
• Insert Size: 2920 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000313.1, NP_000304.1
• RefSeq Size: 3309 bp
• RefSeq ORF: 2031 bp
• Locus ID: 5627
• UniProt ID: P07225
• Cytogenetics: 3q11.1
• Domains: GLA, LamG, EGF_CA, EGF, EGF
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: This gene encodes a vitamin K-dependent plasma protein that functions as a cofactor for the anticoagulant protease, activated protein C (APC) to inhibit blood coagulation. It is found in plasma in both a free, functionally active form and also in an inactive form complexed with C4b-binding protein. Mutations in this gene result in autosomal dominant hereditary thrombophilia. An inactive pseudogene of this locus is located at an adjacent region on chromosome 3. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.