Calcium Sensing Receptor (CASR) (NM_000388) Human Untagged Clone

CAT#: SC119946

CASR (untagged)-Human calcium-sensing receptor (CASR), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Calcium Sensing Receptor
• Synonyms: CAR; EIG8; FHH; FIH; GPRC2A; hCasR; HHC; HHC1; HYPOC1; NSHPT; PCAR1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119946 sequence for NM_000388 edited (data generated by NextGen Sequencing)
  ATGGCATTTTATAGCTGCTGCTGGGTCCTCTTGGCACTCACCTGGCACACCTCTGCCTAC
  GGGCCAGACCAGCGAGCCCAAAAGAAGGGGGACATTATCCTTGGGGGGCTCTTTCCTATT
  CATTTTGGAGTAGCAGCTAAAGATCAAGATCTCAAATCAAGGCCGGAGTCTGTGGAATGT
  ATCAGGTATAATTTCCGTGGGTTTCGCTGGTTACAGGCTATGATATTTGCCATAGAGGAG
  ATAAACAGCAGCCCAGCCCTTCTTCCCAACTTGACGCTGGGATACAGGATATTTGACACT
  TGCAACACCGTTTCTAAGGCCTTGGAAGCCACCCTGAGTTTTGTTGCTCAAAACAAAATT
  GATTCTTTGAACCTTGATGAGTTCTGCAACTGCTCAGAGCACATTCCCTCTACGATTGCT
  GTGGTGGGAGCAACTGGCTCAGGCGTCTCCACGGCAGTGGCAAATCTGCTGGGGCTCTTC
  TACATTCCCCAGGTCAGTTATGCCTCCTCCAGCAGACTCCTCAGCAACAAGAATCAATTC
  AAGTCTTTCCTCCGAACCATCCCCAATGATGAGCACCAGGCCACTGCCATGGCAGACATC
  ATCGAGTATTTCCGCTGGAACTGGGTGGGCACAATTGCAGCTGATGACGACTATGGGCGG
  CCGGGGATTGAGAAATTCCGAGAGGAAGCTGAGGAAAGGGATATCTGCATCGACTTCAGT
  GAACTCATCTCCCAGTACTCTGATGAGGAAGAGATCCAGCATGTGGTAGAGGTGATTCAA
  AATTCCACGGCCAAAGTCATCGTGGTTTTCTCCAGTGGCCCAGATCTTGAGCCCCTCATC
  AAGGAGATTGTCCGGCGCAATATCACGGGCAAGATCTGGCTGGCCAGCGAGGCCTGGGCC
  AGCTCCTCCCTGATCGCCATGCCTCAGTACTTCCACGTGGTTGGCGGCACCATTGGATTC
  GCTCTGAAGGCTGGGCAGATCCCAGGCTTCCGGGAATTCCTGAAGAAGGTCCATCCCAGG
  AAGTCTGTCCACAATGGTTTTGCCAAGGAGTTTTGGGAAGAAACATTTAACTGCCACCTC
  CAAGAAGGTGCAAAAGGACCTTTACCTGTGGACACCTTTCTGAGAGGTCACGAAGAAAGT
  GGCGACAGGTTTAGCAACAGCTCGACAGCCTTCCGACCCCTCTGTACAGGGGATGAGAAC
  ATCAGCAGTGTCGAGACCCCTTACATAGATTACACGCATTTACGGATATCCTACAATGTG
  TACTTAGCAGTCTACTCCATTGCCCACGCCTTGCAAGATATATATACCTGCTTACCTGGG
  AGAGGGCTCTTCACCAATGGCTCCTGTGCAGACATCAAGAAAGTTGAGGCGTGGCAGGTC
  CTGAAGCACCTACGGCATCTAAACTTTACAAACAATATGGGGGAGCAGGTGACCTTTGAT
  GAGTGTGGTGACCTGGTGGGGAACTATTCCATCATCAACTGGCACCTCTCCCCAGAGGAT
  GGCTCCATCGTGTTTAAGGAAGTCGGGTATTACAACGTCTATGCCAAGAAGGGAGAAAGA
  CTCTTCATCAACGAGGAGAAAATCCTGTGGAGTGGGTTCTCCAGGGAGGTGCCCTTCTCC
  AACTGCAGCCGAGACTGCCTGGCAGGGACCAGGAAAGGGATCATTGAGGGGGAGCCCACC
  TGCTGCTTTGAGTGTGTGGAGTGTCCTGATGGGGAGTATAGTGATGAGACAGATGCCAGT
  GCCTGTAACAAGTGCCCAGATGACTTCTGGTCCAATGAGAACCACACCTCCTGCATTGCC
  AAGGAGATCGAGTTTCTGTCGTGGACGGAGCCCTTTGGGATCGCACTCACCCTCTTTGCC
  GTGCTGGGCATTTTCCTGACAGCCTTTGTGCTGGGTGTGTTTATCAAGTTCCGCAACACA
  CCCATTGTCAAGGCCACCAACCGAGAGCTCTCCTACCTCCTCCTCTTCTCCCTGCTCTGC
  TGCTTCTCCAGCTCCCTGTTCTTCATCGGGGAGCCCCAGGACTGGACGTGCCGCCTGCGC
  CAGCCGGCCTTTGGCATCAGCTTCGTGCTCTGCATCTCATGCATCCTGGTGAAAACCAAC
  CGTGTCCTCCTGGTGTTTGAGGCCAAGATCCCCACCAGCTTCCACCGCAAGTGGTGGGGG
  CTCAACCTGCAGTTCCTGCTGGTTTTCCTCTGCACCTTCATGCAGATTGTCATCTGTGTG
  ATCTGGCTCTACACCGCGCCCCCCTCAAGCTACCGCAACCAGGAGCTGGAGGATGAGATC
  ATCTTCATCACGTGCCACGAGGGCTCCCTCATGGCCCTGGGCTTCCTGATCGGCTACACC
  TGCCTGCTGGCTGCCATCTGCTTCTTCTTTGCCTTCAAGTCCCGGAAGCTGCCGGAGAAC
  TTCAATGAAGCCAAGTTCATCACCTTCAGCATGCTCATCTTCTTCATCGTCTGGATCTCC
  TTCATTCCAGCCTATGCCAGCACCTATGGCAAGTTTGTCTCTGCCGTAGAGGTGATTGCC
  ATCCTGGCAGCCAGCTTTGGCTTGCTGGCGTGCATCTTCTTCAACAAGATCTACATCATT
  CTCTTCAAGCCATCCCGCAACACCATCGAGGAGGTGCGTTGCAGCACCGCAGCTCACGCT
  TTCAAGGTGGCTGCCCGGGCCACGCTGCGCCGCAGCAACGTCTCCCGCAAGCGGTCCAGC
  AGCCTTGGAGGCTCCACGGGATCCACCCCCTCCTCCTCCATCAGCAGCAAGAGCAACAGC
  GAAGACCCATTCCCACAGCCCGAGAGGCAGAAGCAGCAGCAGCCGCTGGCCCTAACCCAG
  CAAGAGCAGCAGCAGCAGCCCCTGACCCTCCCACAGCAGCAACGATCTCAGCAGCAGCCC
  AGATGCAAGCAGAAGGTCATCTTTGGCAGCGGCACGGTCACCTTCTCACTGAGCTTTGAT
  GAGCCTCAGAAGAACGCCATGGCCCACAGGAATTCTACGCACCAGAACTCCCTGGAGGCC
  CAGAAAAGCAGCGATACGCTGACCCGACACCAGCCATTACTCCCGCTGCAGTGCGGGGAA
  ACGGACTTAGATCTGACCGTCCAGGAAACAGGTCTGCAAGGACCTGTGGGTGGAGACCAG
  CGGCCAGAGGTGGAGGACCCTGAAGAGTTGTCCCCAGCACTTGTAGTGTCCAGTTCACAG
  AGCTTTGTCATCAGTGGTGGAGGCAGCACTGTTACAGAAAACGTAGTGAATTCATAA
  
  Clone variation with respect to NM_000388.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000388 unedited
  CCATTCAGATTTGTATACGATTCATATAGGCGGCCTGCGAATTCGCACCAGCTTTCGCGC
  TAGAGAGTGGAAGGAGGGAGCTGTTTGCCAGCACCGAGGTCTTGCGGCACAGGCAACGCT
  TGACCTGAGTCTTGCAGAATGAAAGGCATCACAGGAGGCCTCTGCATGATGTGGCTTCCA
  AAGACTCAAGGACCACCCACATTACAAGTCTGGATTGAGGAAGGCAGAAATGGAGATTCA
  AACACCACGTCTTCTATTATTTTATTAATCAATCTGTAGACATGTGTCCCCACTGCAGGG
  AGTGAACTGCTCCAAGGGAGAAACTTCTGGGAGCCTCCAAACTCCTAGCTGTCTCATCCC
  TTGCCCTGGAGAGACGGCAGAACCATGGCATTTTATAGCTGCTGCTGGGTCCTCTTGGCA
  CTCACCTGGCACACCTCTGCCTACGGGCCAGACCAGCGAGCCCAAAAGAAGGGGGACATT
  ATCCTTGGGGGGCTCTTTCCTATTCATTTTGGAGTAGCAGCTAAAGATCAAGATCTCAAA
  TCAAGGCCGGAGTCTGTGGAATGTATCAGGTATAATTTCCGTGGGTTTCGCTGGTTACAG
  GCTATGATATTTGCCATAGAGGAGATAAACAGCAGCCCAGCCCTTCTTCCACACTTGACG
  CTGGGATACAGGATATTTGACACTTGCAACACCGTTTCTAAGGCCTTGGAAGCCACCCTG
  AGTTTTGTTGCTCAGAACAAAATTGATTCTTTGAACCTTGATGAGTTCTGCAACTGCTCA
  GAGCACATTCCCTCTACGATTGCTGTGGTGGGAGCAACTGGCTCAGGCGTCTCCCGGCAG
  TGGCACTCTGCTGGNGCTCTTCTACATCCCCNAGTCAGACTTGCTNCTNCAGCAGATNCT
  CAGCACTAGATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000388 unedited
  AAATACGTTGAACCGCGGCCCGCTATCTACGAGCGNGGNATTTTTTTTTTTTTTTTTTGC
  TCTTCTTTTTTTTTAATTTTAAAGGGAACATGGGTCAATTCACTGTCATTTAAGATGGTG
  TGACTAAATTCGGGGCATTTGATGTGTCTATTATCCCTTCTTCCTCAGAGGAAAGGAGTC
  TGGGGCGATTCCTCATCCCTGGGACCCCAAGAAACCTCTCTGCATTCTCCCTAGCCCAGG
  CTTCTCCTTCCATTTTATGAATTCACTACGTTTTCTGTAACAGTGCTGCCTCCACCACTG
  ATGACAAAGCTCTGTGAACTGGACACTACAAGTGCTGGGGACAACTCTTCAGGGTCCTCC
  ACCTCTGGCCGCTGGTCTCCACCCACAGGTCCTTGCAGACCTGTTTCCTGGACGGTCAGA
  TCTAAGTCCGTTTCCCCGCACTGCAGCGGGAGTAATGGCTGGTGTCGGGTCAGCGTATCG
  CTGCTTTTCTGGGCCTCCAGGGAGTTCTGGTGCGTAAAATTCCTGTGGGCCATGGCGTTC
  TTCTGAGGCTCATCAAAGCTCAGTGAGAAGGTGACCGTGCCGCTGCCAAAGATGACCTTC
  TGCTTGCATCTGGGCTGCTGCTGAGATCGTTGCTGCTGTGGGAGGGTCAGGGGCTGCTGC
  TGCTGCTCTTGCTGGGATAGGGCCAACGGCTGCTGCTGCTTCTGCCTCTCGGGCTGTGGG
  AATGGGTCTTCGCTGTTGCTCTTGCTGCTGATGGAGGAGAGGGGGGTTGGATCCTCGTGG
  AGCCTCCTAGGCTGCTGNACCGCTTGCTGGAAGACGTTGCTGCNGCGCCACCGTGCCCTG
  GCAGCCACTTTAAAGCGTGAGCTGCGGTGCTGCACGCACTCCCCCAGGTGTTTCCGGAAT
  GGCTTAAAAGAGGAAGTNCATTTGTTGAAAAAC
  
• Restriction Sites: Please inquire
• ACCN: NM_000388
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000388.2, NP_000379.2
• RefSeq Size: 4913 bp
• RefSeq ORF: 3237 bp
• Locus ID: 846
• UniProt ID: P41180
• Cytogenetics: 3q13.33-q21.1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: The protein encoded by this gene is a plasma membrane G protein-coupled receptor that senses small changes in circulating calcium concentration. The encoded protein couples this information to intracellular signaling pathways that modify parathyroid hormone secretion or renal cation handling, and thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene are a cause of familial hypocalciuric hypercalcemia, neonatal severe hyperparathyroidism, and autosomal dominant hypocalcemia. [provided by RefSeq, Aug 2017]
  Transcript Variant: This variant (2) has an alternate 5' UTR exon and lacks an in-frame segment in the CDS, as compared to variant 1. The resulting isoform (2) is shorter and lacks an internal segment, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.