Neuraminidase (NEU1) (NM_000434) Human Untagged Clone

CAT#: SC119888

NEU1 (untagged)-Human sialidase 1 (lysosomal sialidase) (NEU1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Neuraminidase
• Synonyms: NANH; NEU; SIAL1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119888 sequence for NM_000434 edited (data generated by NextGen Sequencing)
  ATGACTGGGGAGCGACCCAGCACGGCGCTCCCGGACAGACGCTGGGGGCCGCGGATTCTG
  GGCTTCTGGGGAGGCTGTAGGGTTTGGGTGTTTGCCGCGATCTTCCTGCTGCTGTCTCTG
  GCAGCCTCCTGGTCCAAGGCTGAGAACGACTTCGGTCTGGTGCAGCCGCTGGTGACCATG
  GAGCAACTGCTGTGGGTGAGCGGGAGACAGATCGGCTCAGTGGACACCTTCCGCATCCCG
  CTCATCACAGCCACTCCGCGGGGCACTCTTCTCGCCTTTGCTGAGGCGAGGAAAATGTCC
  TCATCCGATGAGGGGGCCAAGTTCATCGCCCTGCGGAGGTCCATGGACCAGGGCAGCACA
  TGGTCTCCTACAGCGTTCATTGTCAATGATGGGGATGTCCCCGATGGGCTGAACCTTGGG
  GCAGTAGTGAGCGATGTTGAGACAGGAGTAGTATTTCTTTTCTACTCCCTTTGTGCTCAC
  AAGGCCGGCTGCCAGGTGGCCTCTACCATGTTGGTATGGAGCAAGGATGATGGTGTTTCC
  TGGAGCACACCCCGGAATCTCTCCCTGGATATTGGCACTGAAGTGTTTGCCCCTGGACCG
  GGCTCTGGTATTCAGAAACAGCGGGAGCCACGGAAGGGCCGCCTCATCGTGTGTGGCCAT
  GGGACGCTGGAGCGGGACGGAGTCTTCTGTCTCCTCAGCGATGATCATGGTGCCTCCTGG
  CGCTACGGAAGTGGGGTCAGCGGCATCCCCTACGGTCAGCCCAAGCAGGAAAATGATTTC
  AATCCTGATGAATGCCAGCCCTATGAGCTCCCAGATGGCTCAGTCGTCATCAATGCCCGA
  AACCAGAACAACTACCACTGCCACTGCCGAATTGTCCTCCGCAGCTATGATGCCTGTGAT
  ACACTAAGGCCCCGTGATGTGACCTTCGACCCTGAGCTCGTGGACCCTGTGGTAGCTGCA
  GGAGCTGTAGTCACCAGCTCCGGCATTGTCTTCTTCTCCAACCCAGCACATCCAGAGTTC
  CGAGTGAACCTGACCCTGCGATGGAGCTTCAGCAATGGTACCTCATGGCGGAAAGAGACA
  GTCCAGCTATGGCCAGGCCCCAGTGGCTATTCATCCCTGGCAACCCTGGAGGGCAGCATG
  GATGGAGAGGAGCAGGCCCCCCAGCTCTACGTCCTGTATGAGAAAGGCCGGAACCACTAC
  ACAGAGAGCATCTCCGTGGCCAAAATCAGTGTCTATGGGACACTCTGA
  
  Clone variation with respect to NM_000434.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000434 unedited
  TCCTATAGGCGGCCCGCGAATTCGCACGAGGCTGTGGAGTCTAGCTGCCAGGGTCGCGGC
  AGCTGCGGGGAGAGATGACTGGGGAGCGACCCAGCACGGCGCTCCCGGACAGACGCTGGG
  GGCCGCGGATTCTGGGCTTCTGGGGAGGCTGTAGGGTTTGGGTGTTTGCCGCGATCTTCC
  TGCTGCTGTCTCTGGCAGCCTCCTGGTCCAAGGCTGAGAACGACTTCGGTCTGGTGCAGC
  CGCTGGTGACCATGGAGCAACTGCTGTGGGTGAGCGGGAGACAGATCGGCTCAGTGGACA
  CCTTCCGCATCCCGCTCATCACAGCCACTCCGCGGGGCACTCTTCTCGCCTTTGCTGAGG
  CGAGGAAAATGTCCTCATCCGATGAGGGGGCCAAGTTCATCGCCCTGCGGAGGTCCATGG
  ACCAGGGCAGCACATGGTCTCCTACAGCGTTCATTGTCAATGATGGGGATGTCCCCGATG
  GGCTGAACCTTGGGGCAGTAGTGAGCGATGTTGAGACAGGAGTAGTATTTCTTTTCTACT
  CCCTTTGTGCTCACAAGGCCGGCTGCCAGGTGGCCTCTACCATGTTGGTATGGAGCAAGG
  ATGATGGTGTTTCCTGGAGCACACCCCGGAATCTCTCCCTGGATATTGGCACTGAAGTGT
  TTGCCCCTGGACCGGGCTCTGGTATTCAGAAACAGCGGGAGCCACGGAAAGGCCGGCTCA
  TCGTGTGTGGCCATGGGACGCTGGAGCGGNACGGAGTCTTCTGTCTCCTCAGCGATGATC
  ATGGTGCCTCCTGGCGCTCGGAAAGTGGGGTCAGCGGCATCCCCTACGGGCAGCCCAGCA
  CGAAAATGGATTCATTCCTGAGATGGCAGCCCTATAACTCCAGAGGGGTACAGCGTATCA
  TGCCCGAANCAAACACTACCCTGCCACTGCCGAATGCCTCGCAGCTTGAGCCTGGATAAC
  TAGCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000434 unedited
  GGTACATCTATGNNACCGCGGCCGCAATCTANATCGAGTTTTTTTTTTTTTTTTTTAAAA
  TGAAGTCTTGCTCTGTCGCCCAGGCTGGAGGGCAGTGGCATAATCTCGGCTCACTGCAAC
  CTCTGCCTCCCAGGTTCAAGTGAGTCTCCTGCCTCAGGCTCCCGAGTAGCTGGGACTACA
  GGCGTGTGCCACCACATATGGCTAATATTTGTATTTTTATTTTTTATGGGGTTTTACCAT
  GTTGGCCAGGCTGGTCTAGAACCCCTGACCTTGTGATCCGCCCACCTCGGCCTCCCAAAG
  TGCTGGGATTACAGGCGTGAGCCACCGCACCTGACCCTTCCCTGTGTATTAAAAGAAAAA
  AAAAAAGCTGGAAAAAAAAGGTTCTTTAACTATTTCTGCAACTTTGACGTACATATAATT
  CATTTTAGCTGGACACTTGCACTTGTTTAAAAGTTCTGACCCTGGTTTTCAAACTTAAAC
  GTATTACGAATCACCCAGAAGGCTTGTTAATGCCTGGTGGCTCCAACACCAGAGCTTCAG
  ATTCCATGGGTCTGTAAAGAGTGAGGGAGGGAAGGTCAAGCTTTTTTTCTTTCTTGAAGG
  TTTTTTGTTTTGGTTTGGTTTTTTGGAGATGAGGTCTCACTCTGTCACCTAAGTTGGTGT
  GCAGTGGTGCAATCATAGCTTACCACTGCCTCGAACTCCTGGGGTCAAAGAGATCAAGCC
  TTCCCTCCCTGTAGCTAGGAACTTTAGGTGTGCGTTACCCATGCCTGGCTATATTTTTTA
  AATTTTTTAAAACAGGGGGTAATGCCCTTGTTGCCCAAGAAGCCCCTTTAATTTGGATCA
  TCCCGCCCTTGGGCTTCCCGAAGTGCTTGCCTTTCCAATTCTTGAAACCCCCCCCCTAAC
  CCCGGAAGGTAGGTGTTTGTCTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000434
• Insert Size: 3370 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000434.2, NP_000425.1
• RefSeq Size: 1943 bp
• RefSeq ORF: 1248 bp
• Locus ID: 4758
• UniProt ID: Q99519
• Cytogenetics: 6p21.33
• Domains: BNR
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome, Other glycan degradation, Sphingolipid metabolism
• Gene Summary: The protein encoded by this gene is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (the latter is also referred to as 'protective protein'). Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red spot-myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity. [provided by RefSeq, Jul 2008]