HBA1 (NM_000558) Human Untagged Clone

CAT#: SC119836

HBA1 (untagged)-Human hemoglobin, alpha 1 (HBA1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HBA1
• Synonyms: ECYT7; HBA-T3; HBH; METHBA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119836 sequence for NM_000558 edited (data generated by NextGen Sequencing)
  ATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCG
  CACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACC
  AAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGC
  AAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCG
  CTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAG
  CTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCT
  GCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAA
  TACCGTTAA
  
  Clone variation with respect to NM_000558.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000558 unedited
  NGTAAAGTTCAAAATTGTAATACAACTCATATAGGCGGCCCGCGAATTCGCACGAGGCCA
  CCATGGTGCTGTCTCCTGCCGACAANACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCG
  CGCACGCCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACC
  ACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCAC
  GGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAAC
  GCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTC
  AAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACC
  CCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCC
  AAATACCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCCTGCCCGCTGGGCCTCCCAACGG
  GCCCTCCTCCCCTCCTTGCACCGGCCCTTCCTGGTCTTTGAATAAAGTCTGAGTGGGCGG
  CAAANAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTT
  CCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGG
  AAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGT
  CTGACTAGGTGTCCTTCTATATATATGGGTGAGGGGGGGGGTTNNTNNNNNANACNAGGG
  GGNNNNTTGGGAAAAAAAACCCGGTGGGGCCCGCGGGGTCCTATTGGGAACAAGCCTGGA
  TGCAGTGGCACAATCTTGGCCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000558 unedited
  GGCCGCATTCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTGCCGCCCACTCAGACTT
  TATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAG
  CGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTG
  CTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGG
  AGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAAGAGCTTGAAGTTGACCGGGTCC
  ACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAAGGCGGACAGCGCGTTGGGCATGTCGTCC
  ACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGG
  GCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGG
  AACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGCCGACCTTACCCCAG
  GCGGCCTTGACGTTGGTCTTGTCGGCAGGAGACAGCACCATGGTGGCCTCGTGCCGAATT
  CGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTG
  CTTATATAGACCTNCCACCGTACACGCCTACCGGCCATTTGCGTCAACCGGGCGGGGTTA
  TTACCACATTTTGGAAAAGTCCGTTGATTTTGGTGCCCAAACCAACTCCCATTGACGTCA
  ATGGGGGGGAGACTTGGAAATCCCCTGAGTCAACCCTTTTCCCGCCCATTGTGGTCCGCC
  CAACCCGCTCCCCTGGGATACCGAGACTATACCTACAGTCCTGCCAATAGGAAGGCCCTT
  AGGGCTTTACTGGCATATGCCAGCGGCGCATTACACGCTTGACCAAATAGGGCGGACTTG
  CCATGAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000558
• Insert Size: 590 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000558.3, NP_000549.1
• RefSeq Size: 576 bp
• RefSeq ORF: 429 bp
• Locus ID: 3039
• UniProt ID: P69905
• Cytogenetics: 16p13.3
• Domains: globin
• Gene Summary: The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq, Jul 2008]