CD59 (NM_000611) Human Untagged Clone

CAT#: SC119785

CD59 (untagged)-Human CD59 molecule, complement regulatory protein (CD59), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD59
• Synonyms: 1F5; 16.3A5; EJ16; EJ30; EL32; G344; HRF-20; HRF20; MAC-IP; MACIF; MEM43; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21; p18-20
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119785 sequence for NM_000611 edited (data generated by NextGen Sequencing)
  ATGGGAATCCAAGGAGGGTCTGTCCTGTTCGGGCTGCTGCTCGTCCTGGCTGTCTTCTGC
  CATTCAGGTCATAGCCTGCAGTGCTACAACTGTCCTAACCCAACTGCTGACTGCAAAACA
  GCCGTCAATTGTTCATCTGATTTTGATGCGTGTCTCATTACCAAAGCTGGGTTACAAGTG
  TATAACAAGTGTTGGAAGTTTGAGCATTGCAATTTCAACGACGTCACAACCCGCTTGAGG
  GAAAATGAGCTAACGTACTACTGCTGCAAGAAGGACCTGTGTAACTTTAACGAACAGCTT
  GAAAATGGTGGGACATCCTTATCAGAGAAAACAGTTCTTCTGCTGGTGACTCCATTTCTG
  GCAGCAGCCTGGAGCCTTCATCCCTAA
  
  Clone variation with respect to NM_000611.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000611 unedited
  GGTGCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGAGGATCCT
  GGGCGCCGCCAGTCTTTAGCACCAGTTGGTGTAGGAGTTGAGACCTACTTCACAGTAGTT
  CTGTGGACAATCACAATGGGAATCCAAGGAGGGTCTGTCCTGTTCGGGCTGCTGCTCGTC
  CTGGCTGTCTTCTGCCATTCAGGTCATAGCCTGCAGTGCTACAACTGTCCTAACCCAACT
  GCTGACTGCAAAACAGCCGTCAATTGTTCATCTGATTTTGATGCGTGTCTCATTACCAAA
  GCTGGGTTACAAGTGTATAACAAGTGTTGGAAGTTTGAGCATTGCAATTTCAACGACGTC
  ACAACCCGCTTGAGGGAAAATGAGCTAACGTACTACTGCTGCAAGAAGGACCTGTGTAAC
  TTTAACGAACAGCTTGAAAATGGTGGGACATCCTTATCAGAGAAAACAGTTCTTCTGCTG
  GTGACTCCATTTCTGGCAGCAGCCTGGAGCCTTCATCCCTAAGTCAACACCAGGAGAGCT
  TCTCCCAAACTCCCCGTTCCTGCGTAGTCCGCTTTCTCTTGCTGCCACATTCTAAAGGCT
  TGATATTTTCCAAATGGATCCTGTTGGGAAAGAATAAAATTAGCTTGAGCAACCTGNCTA
  AGATAGAGGGGCTCTGGGAGACTTTGAAGACCAGTCCTGTTTGCAGGGAAGCCCCACTTG
  AAGAAAGAAGTCTAAGAGTGAAGTANGTGTGACTTGAACTAGATTGCATGCTTCCTCCTT
  TGCTCTTGGGAAGACCACTNTGCAGTGACAGCTTGAGTGGGGTTCTCTGCAGCCCTCAGA
  TTATTTTTCCTCCTGCTCCTTGNATGTAGCCAGTTAGCATCATTAGTACATCTTTGAAGG
  TGGGGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000611 unedited
  NAATCCTAGCTATGNCCGCGGCCGCTATCTAGATCGAGTTTTTTTTTTTTTTTTTTCGGT
  TTGTCCAACAGATATAATCAAGGGTTTTTTTTCAACATTAATCAAGAAATTCCTCCATCT
  GCAAAAGTCAGCCTATGCCATTAAATACAGCCAAGATCATAAAATACAAATGTCATTAGG
  TCTACTGAATCTTGAGATTCATTCACTAGAATACACAGGGAAAAGGAAAAAGTTTGCCCT
  TGTGAAAGGTGTGTATCATTTTTTAAAAGAGCTTCTGAAAGCCTCAAACATCTGTGAGTG
  TGCTAAGAGCCGTTACAAAGGTATTTTATACAATCTATGCTGAGTCAGCTAGGAGTTAGC
  AGGAGGCTGGATGCAGATGGTAAAACATACCCCTGCAGATCTGTTGTTATTAAGGATGAC
  CTTTGGAAGTCTCAAACTGTAACCACAAAGGGTGTCCCCACTGCATTCATACTATATACA
  CACATACCTGAACTGCCTTTCTACCAGTAACATTTAAAATAAGCACACTTAATACTGCCA
  GTCACATGTAGTAGAGTGTTCACTCTGTAACTGTGTGTGGCAAAGCCAAGATATCCTACC
  ATTCCAATAAAAAAAAGGCAAGGAAATAGCTTTAACACACCATTGATGTATGCTATTACA
  CTTTTCCAGTGGAAGATAACAAAGCCCATATAAAATTTAAAGACAGAGGAAAAACTCGAT
  TTTCAGCCACTTGTACCTCTCTAAGTTTTCATGCCCTGCTATCTGGAACACTTCCCCACT
  TCCCTGCCAGAAGTCCTCTAGCTCTGCATTAAGGGATCTGNTGTCCCTGACTGACACCCC
  ATATGGAACATTTGCCATGCCTNCATGCACACGGGATCCCTGAAGTTATGAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000611
• Insert Size: 1900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000611.4, NP_000602.1
• RefSeq Size: 7633 bp
• RefSeq ORF: 387 bp
• Locus ID: 966
• UniProt ID: P13987
• Cytogenetics: 11p13
• Domains: LU
• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades, Hematopoietic cell lineage
• Gene Summary: This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All variants (1-8) encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.