ADH1B (NM_000668) Human Untagged Clone

CAT#: SC119751

ADH1B (untagged)-Human alcohol dehydrogenase 1B (class I), beta polypeptide (ADH1B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADH1B
• Synonyms: ADH2; HEL-S-117
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119751 sequence for NM_000668 edited (data generated by NextGen Sequencing)
  ATGAGCACAGCAGGAAAAGTAATCAAATGCAAAGCAGCTGTGCTATGGGAGGTAAAGAAA
  CCCTTTTCCATTGAGGATGTGGAGGTTGCACCTCCTAAGGCTTATGAAGTTCGCATTAAG
  ATGGTGGCTGTAGGAATCTGTCGCACAGATGACCACGTGGTTAGTGGCAACCTGGTGACC
  CCCCTTCCTGTGATTTTAGGCCATGAGGCAGCCGGCATCGTGGAGAGTGTTGGAGAAGGG
  GTGACTACAGTCAAACCAGGTGATAAAGTCATCCCGCTCTTTACTCCTCAGTGTGGAAAA
  TGCAGAGTTTGTAAAAACCCGGAGAGCAACTACTGCTTGAAAAATGATCTAGGCAATCCT
  CGGGGGACCCTGCAGGATGGCACCAGGAGGTTCACCTGCAGGGGGAAGCCCATTCACCAC
  TTCCTTGGCACCAGCACCTTCTCCCAGTACACGGTGGTGGATGAGAATGCAGTGGCCAAA
  ATTGATGCAGCCTCGCCCCTGGAGAAAGTCTGCCTCATTGGCTGTGGATTCTCGACTGGT
  TATGGGTCTGCAGTTAACGTTGCCAAGGTCACCCCAGGCTCTACCTGTGCTGTGTTTGGC
  CTGGGAGGGGTCGGCCTATCTGCTGTTATGGGCTGTAAAGCAGCTGGAGCAGCCAGAATC
  ATTGCGGTGGACATCAACAAGGACAAATTTGCAAAGGCCAAAGAGTTGGGTGCCACTGAA
  TGCATCAACCCTCAAGACTACAAGAAACCCATCCAGGAAGTGCTAAAGGAAATGACTGAT
  GGAGGTGTGGATTTTTCGTTTGAAGTCATCGGTCGGCTTGACACCATGATGGCTTCCCTG
  TTATGTTGTCATGAGGCATGTGGCACAAGCGTCATCGTAGGGGTACCTCCTGCTTCCCAG
  AACCTCTCAATAAACCCTATGCTGCTACTGACTGGACGCACCTGGAAGGGGGCTGTTTAT
  GGTGGCTTTAAGAGTAAAGAAGGTATCCCAAAACTTGTGGCTGATTTTATGGCTAAGAAG
  TTTTCACTGGATGCGTTAATAACCCATGTTTTACCTTTTGAAAAAATAAATGAAGGATTT
  GACCTGCTTCACTCTGGGAAAAGTATCCGTACCGTCCTGACGTTTTGA
  
  Clone variation with respect to NM_000668.4
  143 a=>g;753 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000668 unedited
  TCTTGGGATTTTGTAATCCCACTTTACTATAGGGCGGCACACACATTCGGCACGAGGCAA
  AGACTCACAGTCTGCTGGTGGGCAGAGAAGACAGAAACGACATGAGCACAGCAGGGAAAA
  GTAATCAAATGCAAAGCAGCTGTGCTATGGGAGGTAAAGAAACCCTTTTCCATTGAGGAT
  GTGGAGGTTGCACCTCCTAAGGCTTATGAAGTTCGCATTATTTATGGTGGCTGTAGGAAT
  CTGTCGCACAGATGACCACGTGGTTAGTGGCAACCTGGTGACCCCCCTTCCTGTGATTTT
  AGGCCATGAGGCAGCCGGCATCGTGGAGAGTGTTGGAGAAGGGGTGACTACAGTCAAACC
  AGGTGATAAAGTCATCCCGCTCTTTACTCCTCAGTGTGGAAAATGCAGAGTTTGTAAAAA
  CCCGGAGAGCAACTACTGCTTGAAAAATGATCTAGGCAATCCTCGGGGGACCCTGCAGGA
  TGGCACCAGGAGGTTCACCTGCAGGGGGAAGCCCATTCACCACTTCCTTGGCACCAGCAC
  CTTCTCCCAGTACACGGTGGTGGATGAGAATGCAGTGGCCAAAATTGATGCAGCCTCGCC
  CCTGGAGAAAGTCTGCCTCATTGGCTGTGGATTCTCGACTGGTTATGGGTCTGCAGTTAA
  CGTTGCCAATGTCACCCCANGCTCTACCTGTGCTGTGTTTGGCCTGAGGAGGGTCGGCCT
  ATCTGCTGTTATGGGCTGTNAAGCAGCTGGAGCAGCCAGAATCATTGCGGTGGACATNNC
  ACAGGGACAATTTTGCAAGGCCAAGAGTTGGGTGCCACTGAATGCATCACCCCTCAGACT
  ACAGGATACCATCCAGNAAGTGCTAAAGGAATGACTNGATGGAGTGTGGATTTTCGTTTG
  AAGTCATCGTCGGCTTGCACCATGAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000668 unedited
  CTATGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAAGAAACACAG
  TAAATGATAAACTATTTTACTTTATGTCTAAGGTCTTTCATAATATGAAATAGAATGTAG
  ATATTGCAACAATAGCATTTTTGGAGACAGCTACCTCCTTTACCAGGAATAATCTTTGCA
  TGTCACATTTAGAGATAAAGCTCAAAATGCAAATCCTTCCCCTGAGAGTGGGAAAGCATT
  AACAAATGAGAGTGGGAAAAGCATTAACAAAGCATTAACACAGGTCTTTACATATTCAAA
  ATATTAAACTAATGCTAGGATTATAGACTTGATTTTAAGACATGGTAGTTAATAGAAAAG
  TTCTAGATTGAAAACAATTTTGCAAAAATATACATTTGTATATGTGTATATATGTATGTG
  TATATATATATCTACTAGGAAAATATATTGCTTAAGGTGTTTGTGCCATTTCTTAGTTAC
  CAATTTTACATTCTGCATTCACAGCATTTGCCATCCCATCTCTACACTTAAAGCCACCAT
  ATTACTTTTAATTACTTTATACACATTTTCAGTCATCATCTAAATAATCATTTATAGCCA
  CAAACTAATACTGTTTAGTCAATGAAGCACTTTCATCAGCAAAAATTACAGATTTTTTGA
  ACATGATTCTGGGAATAGTTCAGTTTTTACAATTAGTAATTGAGGCTTTGTCATATGAAG
  CAAGAAATCTGCTATTATTTTAAAAAATCAATTGTATATCGACCATAAAGAAATAGTATA
  AAAGTCATAAAAACACTGCCATTTACTGTAGTAAAATGTGGGGCATTTTATTTGAGTTCC
  TAAGAAATTTCCTGTATTTAGACTCTCGGCCTTCACTACTTGAAATGTGAGTCTATCCTT
  TACCTTACCCTAGCTTTTACCCCAGGGATTTGGTACATTTTTATTAAAAAAAGAAATTGT
  CGACGGTGAAACCGGACAGAGTTTGAAGACTTCATCACATGGGCCACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000668
• Insert Size: 2790 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000668.3, NP_000659.2
• RefSeq Size: 2666 bp
• RefSeq ORF: 1128 bp
• Locus ID: 125
• UniProt ID: P00325
• Cytogenetics: 4q23
• Domains: ADH_zinc_N
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - cytochrome P450, Fatty acid metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism, Tyrosine metabolism
• Gene Summary: The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.