ATP1A3 (NM_000703) Human Untagged Clone

CAT#: SC119716

(untagged)-Human ATPase, Na+/K+ transporting, alpha 3 polypeptide (ATP1A3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP1A3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000703, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGGACAAGAAAGATGACAAGGACTCACCCAAGAAGAACAAGGGCAAGGAGCGCCGGGACCTGGATG
  ACCTCAAGAAGGAGGTGGCTATGACAGAGCACAAGATGTCAGTGGAAGAGGTCTGCCGGAAATACAACAC
  AGACTGTGTGCAGGGTTTGACCCACAGCAAAGCCCAGGAGATCCTGGCCCGGGATGGGCCTAACGCACTC
  ACGCCACCGCCTACCACCCCAGAGTGGGTCAAGTTTTGCCGGCAGCTCTTCGGGGGCTTCTCCATCCTGC
  TGTGGATCGGGGCTATCCTCTGCTTCCTGGCCTACGGTATCCAGGCGGGCACCGAGGACGACCCCTCTGG
  TGACAACCTGTACCTGGGCATCGTGCTGGCGGCCGTGGTGATCATCACTGGCTGCTTCTCCTACTACCAG
  GAGGCCAAGAGCTCCAAGATCATGGAGTCCTTCAAGAACATGGTGCCCCAGCAAGCCCTGGTGATCCGGG
  AAGGTGAGAAGATGCAGGTGAACGCTGAGGAGGTGGTGGTCGGGGACCTGGTGGAGATCAAGGGTGGAGA
  CCGAGTGCCAGCTGACCTGCGGATCATCTCAGCCCACGGCTGCAAGGTGGACAACTCCTCCCTGACTGGC
  GAATCCGAGCCCCAGACTCGCTCTCCCGACTGCACGCACGACAACCCCTTGGAGACTCGGAACATCACCT
  TCTTTTCCACCAACTGTGTGGAAGGCACGGCTCGGGGCGTGGTGGTGGCCACGGGCGACCGCACTGTCAT
  GGGCCGTATCGCCACCCTGGCATCAGGGCTGGAGGTGGGCAAGACGCCCATCGCCATCGAGATTGAGCAC
  TTCATCCAGCTCATCACCGGCGTGGCTGTCTTCCTGGGTGTCTCCTTCTTCATCCTCTCCCTCATTCTCG
  GATACACCTGGCTTGAGGCTGTCATCTTCCTCATCGGCATCATCGTGGCCAATGTCCCAGAGGGTCTGCT
  GGCCACTGTCACTGTGTGTCTGACCGTGACCGCCAAGCGCATGGCCCGGAAGAACTGCCTGGTGAAGAAC
  CTGGAGGCTGTAGAGACCCTGGGCTCCACGTCCACCATCTGCTCAGATAAGACAGGGACCCTCACTCAGA
  ACCGCATGACAGTCGCCCACATGTGGTTTGACAACCAGATCCACGAGGCTGACACCACTGAGGACCAGTC
  AGGGACCTCATTTGACAAGAGTTCGCACACCTGGGTGGCCCTGTCTCACATCGCTGGGCTCTGCAATCGC
  GCTGTCTTCAAGGGTGGTCAGGACAACATCCCTGTGCTCAAGAGGGATGTGGCTGGGGATGCGTCTGAGT
  CTGCCCTGCTCAAGTGCATCGAGCTGTCCTCTGGCTCCGTGAAGCTGATGCGTGAACGAAACAAGAAAGT
  GGCTGAGATTCCCTTCAATTCCACCAACAAATACCAGCTCTCCATCCATGAGACCGAGGACCCCAACGAC
  AACCGATACCTGCTGGTGATGAAGGGTGCCCCCGAGCGCATCCTGGACCGCTGCTCCACCATCCTGCTAC
  AGGGCAAGGAGCAGCCTCTGGACGAGGAAATGAAGGAGGCCTTTCAGAATGCCTACCTTGAGCTCGGTGG
  CCTGGGCGAGCGCGTGCTTGGTTTCTGCCATTATTACCTGCCCGAGGAGCAGTATCCCCAAGGCTTTGCC
  TTCGACTGTGATGACGTGAACTTCACCACGGACAACCTCTGCTTTGTGCCGCTCATGTCCATGATCGGCC
  CACCCCGGGCAGCCGTCCCTGACGCGGTGGGCAAGTGTCGCAGCGCAGGCATCAAGGTCATCATGGTCAC
  CGGCGATCACCCCATCACGGCCAAGGCCATTGCCAAGGGTGTGGGCATCATCTCTGAGGGCAACGAGACT
  GTGGAGGACATCGCCGCCCGGCTCAACATTCCCGTCAGCCAGGTTAACCCCCGGGATGCCAAGGCCTGCG
  TGATCCACGGCACCGACCTCAAGGACTTCACCTCCGAGCAAATCGACGAGATCCTGCAGAATCACACCGA
  GATCGTCTTCGCCCGCACATCCCCCCAGCAGAAGCTCATCATTGTGGAGGGCTGTCAGAGACAGGGTGCA
  ATTGTGGCTGTGACCGGGGATGGTGTGAACGACTCCCCCGCTCTGAAGAAGGCCGACATTGGGGTGGCCA
  TGGGCATCGCTGGCTCTGACGTCTCCAAGCAGGCAGCTGACATGATCCTGCTGGACGACAACTTTGCCTC
  CATCGTCACAGGGGTGGAGGAGGGCCGCCTGATCTTCGACAACCTAAAGAAGTCCATTGCCTACACCCTG
  ACCAGCAATATCCCGGAGATCACGCCCTTCCTGCTGTTCATCATGGCCAACATCCCGCTGCCCCTGGGCA
  CCATCACCATCCTCTGCATCGATCTGGGCACTGACATGGTCCCTGCCATCTCACTGGCGTACGAGGCTGC
  CGAAAGCGACATCATGAAGAGACAGCCCAGGAACCCGCGGACGGACAAATTGGTCAATGAGAGACTCATC
  AGCATGGCCTACGGGCAGATTGGAATGATCCAGGCTCTCGGTGGCTTCTTCTCTTACTTTGTGATCCTGG
  CAGAAAATGGCTTCTTGCCCGGCAACCTGGTGGGCATCCGGCTGAACTGGGATGACCGCACCGTCAATGA
  CCTGGAAGACAGTTACGGGCAGCAGTGGACATACGAGCAGAGGAAGGTGGTGGAGTTCACCTGCCACACG
  GCCTTCTTTGTGAGCATCGTTGTCGTCCAGTGGGCCGATCTGATCATCTGCAAGACCCGGAGGAACTCGG
  TCTTCCAGCAGGGCATGAAGAACAAGATCCTGATCTTCGGGCTGTTTGAGGAGACGGCCCTGGCTGCCTT
  CCTGTCCTACTGCCCCGGAATGGACGTGGCCCTGCGCATGTACCCTCTCAAGCCCAGCTGGTGGTTCTGT
  GCCTTCCCCTACAGTTTCCTCATCTTCGTCTACGACGAAATCCGCAAACTCATCCTGCGCAGGAACCCAG
  GGGGTTCGGTGGAGAAGGAAACCTACTACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000703 unedited
  CCTGGATTTGTAACCGACTTTTATAGGCGGCCACGCATTCGGCACGAGGGACCAACGGAC
  GGACGGACGGACGCGCGCACCTACCGAGGCGCGGGCGCTGCAGAGGCTCCCAGCCCAAGC
  CTGAGCCTGAGCCCGCCCCGAGGTCCCCGCCCCGCCCGCCTGGCTCTCTCGCCGCGGAGC
  CGCCAAGATGGGGGACAAGAAAGATGACAAGGACTCACCCAAGAAGAACAAGGGCAAGGA
  GCGCCGGGACCTGGATGACCTCAAGAAGGAGGTGGCTATGACAGAGCACAAGATGTCAGT
  GGAAGAGGTCTGCCGGAAATACAACACAGACTGTGTGCAGGGTTTGACCCACAGCAAAGC
  CCAGGAGATCCTGGCCCGGGATGGGCCTAACGCACTCACGCCACCGCCTACCACCCCAGA
  GTGGGTCAAGTTTTGCCGGCAGCTCTTCGGGGGCTTCTCCATCCTGCTGTGGATATGGGC
  TATCCTCTGCTTCCTGGCCTACGGTATCCAGGCGGGCACCGAGGACGACCCCTCTGGTGA
  CAACCTGTACCTGGGCATCGTGCTGGCGGCCGTGGTGATCATCACTGGCTGCTTCTCCTA
  CTACCAGGAGGCCAAGAGCTCCAAGATCATGGAGTCCTTCAAGAACATGGTGCCCCAGCA
  AGCCCTGGTGATCCGGGAAGGTGAGAAGATGCANGTGAACGCTGANGANGTGGTGGTCGG
  GGACCTGGTGGAGATCAAGGGTGGAGACCGAGTGCCAGCTGACCTGCNGATCATCTCAGC
  CCACGGCTGCAAGGTGGACAACTCCTNCCTGACTGGCGAATNCGAGCCCCAGACTCGCTC
  TTCCCGACTGCACTCACGACAACCCTTTGGAGACTCCGGACATCACCTTCTTTTCACCAA
  CTGTGTGGAAGGCCACGCTCGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000703 unedited
  TTCCAGGGCCGGTAAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAG
  GAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTC
  TCTCTCCCCACTGATATATTTGATAATTGTCCAAAACCAGAGATGGCATCAGCCGGGGGG
  CTGAAGGGGAGTAAAAAAGAGCCCAGGGAGGTGGCTGGGGCGGGAGGAATGGATAGAGGG
  GTGAGGAGAGGGAGAGAAACTGACACAGAAAAGAGAGAGGAGAGAGGTTTGGGGCAGGGG
  AAAAAACCCAGCCAGAGTGGGGGCGGCAGGAAATAGTGGAGGGGGTGGGGGCCAAGGTGG
  GGCCACAGGAAGAGAGGGCTCCTCCCCCCAGAATACAAAATTGGGGGGACTGACAGGGGC
  GGTCCTGGGCCTGGGGGACGGGGAAGAGATGGGCGATGTGGTGGGGCTGAGGTCANTANT
  AGGTTTCCTTCTCCACCCAACCCCCTGGGTTCCTGCGCAGGATGAGTTTGCGGATTTCGT
  CGTAAACGAAGATGAGGAAACTGTAGGGGAAGGCACAGAACCACCAGCTGGGCTTGAGAG
  GGTACATGCGCAGGGCCACGTCCATGCCGGGGCAGTAGGACAGGAAGGCAACCAGGGGCC
  GTCTCCTCAAACGCCCGAGATCANGGATCTTGTTCTTCATGCCCTGCTGGAGNACCGAGT
  CCCTTCGGGTCTTGCAAATGATCAAATCGGGCCACTGGACGACACGATGCTCACCAAGAG
  GCCGGGTGGAAGGGAACTCCACCCCTTCCTTGGTCGTATGTCCCTGCTGCCCGTACTGTT
  TTCAGGCATTGACGGGGGCGGTTTCCCATTTAACCGGTGCCACCAGTTGTCGGCAAAACC
  CTTTCTGTCCGACCCAAAAAAAAAAAACCCCGAACCCGACTTCCAACCCCCGGGCCCTCT
  GTGATCTCATTCCAATGCCGCCCCGGTTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000703
• Insert Size: 3720 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000703.1, NP_000694.1
• RefSeq Size: 3635 bp
• RefSeq ORF: 3042 bp
• Locus ID: 478
• Cytogenetics: 19q13.2
• Domains: E1-E2_ATPase, Cation_ATPase_N, Hydrolase, Cation_ATPase_C
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cardiac muscle contraction
• Gene Summary: The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 3 subunit. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]