Leukotriene A4 hydrolase (LTA4H) (NM_000895) Human Untagged Clone

CAT#: SC119594

LTA4H (untagged)-Human leukotriene A4 hydrolase (LTA4H)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Leukotriene A4 hydrolase
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119594 sequence for NM_000895 edited (data generated by NextGen Sequencing)
  ATGCCCGAGATAGTGGATACCTGTTCGTTGGCCTCTCCGGCTTCCGTCTGCCGGACCAAG
  CACCTGCACCTGCGCTGCAGCGTCGACTTTACTCGCCGGACGCTGACCGGGACTGCTGCT
  CTCACGGTCCAGTCTCAGGAGGACAATCTGCGCAGCCTGGTTTTGGATACAAAGGACCTT
  ACAATAGAAAAAGTAGTGATCAATGGACAAGAAGTCAAATATGCTCTTGGAGAAAGACAA
  AGTTACAAGGGATCGCCAATGGAAATCTCTCTTCCTATCGCTTTGAGCAAAAATCAAGAA
  ATTGTTATAGAAATTTCTTTTGAGACCTCTCCAAAATCTTCTGCTCTCCAGTGGCTCACT
  CCTGAACAGACTTCTGGGAAGGAACACCCATATCTCTTTAGTCAGTGCCAGGCCATCCAC
  TGCAGAGCAATCCTTCCTTGTCAGGACACTCCTTCTGTGAAATTAACCTATACTGCAGAG
  GTGTCTGTCCCTAAAGAACTGGTGGCACTTATGAGTGCTATTCGTGATGGAGAAACACCT
  GACCCAGAAGACCCAAGCAGGAAAATATACAAATTCATCCAAAAAGTTCCAATACCCTGC
  TACCTGATTGCTTTAGTTGTTGGAGCTTTAGAAAGCAGGCAAATTGGCCCAAGAACTTTG
  GTGTGGTCTGAGAAAGAGCAGGTGGAAAAGTCTGCTTATGAGTTTTCTGAGACTGAATCT
  ATGCTTAAAATAGCAGAAGATCTGGGAGGACCGTATGTATGGGGACAGTATGACCTATTG
  GTCCTGCCACCATCCTTCCCTTATGGTGGCATGGAGAATCCTTGCCTTACTTTTGTAACT
  CCTACTCTACTGGCAGGCGACAAGTCACTCTCCAATGTCATTGCACATGAAATATCTCAT
  AGCTGGACAGGGAATCTAGTGACCAACAAAACTTGGGATCACTTTTGGTTAAATGAGGGA
  CATACTGTGTACTTGGAACGCCACATTTGCGGACGATTGTTTGGTGAAAAGTTCAGACAT
  TTTAATGCTCTGGGAGGATGGGGAGAACTACAGAATTCGGTAAAGACATTTGGGGAGACA
  CATCCTTTCACCAAACTTGTGGTTGATCTGACAGATATAGACCCTGATGTAGCTTATTCT
  TCAGTTCCCTATGAGAAGGGCTTTGCTTTACTTTTTTACCTTGAACAACTGCTTGGAGGA
  CCAGAGATTTTCCTAGGATTCTTAAAAGCTTATGTTGAGAAGTTTTCCTATAAGAGCATA
  ACTACTGATGACTGGAAGGATTTCCTGTATTCCTATTTTAAAGATAAGGTTGATGTTCTC
  AATCAAGTTGATTGGAATGCCTGGCTCTACTCTCCTGGACTGCCTCCCATAAAGCCCAAT
  TATGATATGACTCTGACAAATGCTTGTATTGCCTTAAGTCAAAGATGGATTACTGCCAAA
  GAAGATGATTTAAATTCATTCAATGCCACAGACCTGAAGGATCTCTCTTCTCATCAATTG
  AATGAGTTTTTAGCACAGACGCTCCAGAGGGCACCTCTTCCATTGGGGCACATAAAGCGA
  ATGCAAGAGGTGTACAACTTCAATGCCATTAACAATTCTGAAATACGATTCAGATGGCTG
  CGGCTCTGCATTCAATCCAAGTGGGAGGACGCAATTCCTTTGGCGCTAAAGATGGCAACT
  GAACAAGGAAGAATGAAGTTTACCCGGCCCTTATTCAAGGATCTTGCTGCCTTTGACAAA
  TCCCATGATCAAGCTGTCCGAACCTACCAAGAGCACAAAGCAAGCATGCATCCCGTGACT
  GCAATGCTGGTGGGGAAAGACTTAAAAGTGGATTAA
  
  Clone variation with respect to NM_000895.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000895 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTGGGCTGTAGGTC
  GCTGTGCTGTGTGATCCCCCAGAGCCATGCCCGAGATAGTGGATACCTGTTCGTTGGCCT
  CTCCGGCTTCCGTCTGCCGGACCAAGCACCTGCACCTGCGCTGCAGCGTCGACTTTACTC
  GCCGGACGCTGACCGGGACTGCTGCTCTCACGGTCCAGTCTCAGGAGGACAATCTGCGCA
  GCCTGGTTTTGGATACAAAGGACCTTACAATAGAAAAAGTAGTGATCAATGGACAAGAAG
  TCAAATATGCTCTTGGAGAAAGACAAAGTTACAAGGGATCGCCAATGGAAATCTCTCTTC
  CTATCGCTTTGAGCAAAAATCAAGAAATTGTTATAGAAATTTCTTTTGAGACCTCTCCAA
  AATCTTCTGCTCTCCAGTGGCTCACTCCTGAACAGACTTCTGGGAAGGAACACCCATATC
  TCTTTAGTCAGTGCCAGGCCATCCACTGCAGAGCAATCCTTCCTTGTCAGGACACTCCTT
  CTGTGAAATTAACCTATACTGCAGAGGTGTCTGTCCCTAAAGAACTGGTGGCACTTATGA
  GTGCTATTCGTGATGGAGAAACACCTGACCCAGAAGACCCAAGCAGGANAATATACAAAT
  TCATCCAAAAAGTTCCAATACCCTGCTACCTGATTGCTTTAGTTGTTGGAGCTTTAGAAA
  GCAGGCAAATTGGCCCAAGAACTNTGGGTGTGGTCTGAGAAAGAGCANGTGGAAAAGTCT
  GCTTATGAGTTTTCTGAGACTGAATCTATGCTTAAAAAGCANAAGATCTGGNGAGACCGT
  ATGTATGGGGACAGTATGACCTATTGGTCCTGCCACATCCTTCCCTATGTNGCATGGAGA
  TTCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000895 unedited
  CTCAATTCTATTTTTTTTAAAATCACCAACAAAACATAAAAAGCCAACTAAAAAGACAGT
  TTTAATTGTGAGCTGAAGTTTTATATTTCTTTACGAATTCCATTTAAAAAAGAGAAATCT
  CTAAAATCATCAATACGCAGGTCTTTAATCCACTTTTAAGTCTTTCCCCACCAGCATTGC
  AGTCACGGGATGCATGCTTGCTTTGTGCTCTTGGTAGGTTCGGACAGCTTGATCATGGGA
  TTTGTCAAAGGCAGCAAGATCCTTGAATAAGGGCCGGGTAAACTTCATTCTTCCTTGTTC
  AGTTGCCATCTTTAGCGCCAAAGGAATTGCGTCCTCCCACTTGGATTGAATGCAGAGCCG
  CAGCCATCTGAATCGTATTTCAGAATTGTTAATGGCATTGAAGCCGTACACCTCTTGCAT
  TCGCTTTATGTGCCCCAATGGAAGACGTGCCCTCTGGAGCGCCCGCGCTAAAAACTCATT
  CACTTGATGAGAAAATAGAACCTTCAAGTCTCGCGGCATTGCATGAATCTAACACATCTC
  TTTCTGGCGAGACATCCATCTTTCGCTCAAGGGCCTAGTCGCCTCTGTCATAGCCATCTT
  ATTATCTCGGCCTTACTGTGTCGCACTCCACGGACACGTAGAGTCATGTCCTTCAATCCC
  CCCGCATGTGAACATGCACCTCATCCCTACACATGGACCCCGGACATCTCCCCGTCACTA
  ATCATTTGCGTGCATCGGGATGCCCTCCTACCACACCTCTAATAATACACTGAACACCTT
  TGTCCTCCTCTCTGTTTCACCGGATTCAGTAGCTACGACCGTTTTACGTGTCCTGCTTAC
  CGCTTCCGTCGCGCCTTTCCGCCCGACCATTCATTCTTTTCCACTCATTATCTCCCCCCG
  CCTCGCTTAACNTCGNNATCATCCATCCCAATTACCCCACGCCTCGCATTGGCCAAACAT
  CCACCACATGCGCCCCTCCCCACTATGCCACCTCATACTCCCTGTAGCCACCATATCGCC
  T
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000895
• Insert Size: 2230 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000895.1, NP_000886.1
• RefSeq Size: 2060 bp
• RefSeq ORF: 1836 bp
• Locus ID: 4048
• UniProt ID: P09960
• Cytogenetics: 12q23.1
• Domains: Peptidase_M1
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• Gene Summary: The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1) encodes the longest isoform (1).