RPS17 (NM_001021) Human Untagged Clone

CAT#: SC119528

RPS17 (untagged)-Human ribosomal protein S17 (RPS17)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS17
• Synonyms: DBA4; RPS17L; RPS17L1; RPS17L2; S17
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119528 sequence for NM_001021 edited (data generated by NextGen Sequencing)
  ATGGGCCGCGTTCGCACCAAAACCGTGAAGAAGGCGGCCCGGGTCATCATAGAAAAGTAC
  TACACGCGCCTGGGCAACGACTTCCACACGAACAAGCGCGTGTGCGAGGAGATCGCCATT
  ATCCCCAGCAAAAAGCTCCGCAACAAGATAGCAGGTTACGTCACGCATCTGATGAAGCGA
  ATTCAGAGAGGCCCAGTAAGAGGTATCTCCATCAAGCTGCAGGAGGAGGAGAGAGAAAGG
  AGAGACAATTATGTTCCTGAGGTCTCAGCCTTGGATCAGGAGATTATTGAAGTAGATCCT
  GACACTAAGGAAATGCTGAAGCTTTTGGACTTCGGCAGTCTGTCCAACCTTCAGGTCACT
  CAGCCTACAGTTGGGATGAATTTCAAAACGCCTCGGGGACCTGTTTGA
  
  Clone variation with respect to NM_001021.3
  159 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001021 unedited
  TATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCCAACATGGGC
  CGCGTTCGCACCAAAACCGTGAAGAAGCGGCCCGGGTCATCATAGAAAAGTACTACACGC
  GCCTGGGCAACGACTTCCACACGAACAAGCGCGTGTGCGAGGAGATCGCCATTATCCCCA
  GCAAAAAGCTCCGCAACAAGATAGCAGGTTACGTCACGCATCTGATGAAGCGAATTCAGA
  GAGGCCCAGTAAGAGGTATCTCCATCAAGCTGCAGGAGGAGGAGAGAGAAAGGAGAGACA
  ATTATGTTCCTGAGGTCTCAGCCTTGGATCAGGAGATTATTGAAGTAGATCCTGACACTA
  AGGAAATGCTGAAGCTTTTGGACTTCGGCAGTCTGTCCAACCTTCAGGTCACTCAGCCTA
  CAGTTGGGATGAATTTCAAAACGCCTCGGGGACCTGTTTGAATTTTTTCTGTAGTGCTGT
  ATTATTTTCAATAAATCTGGGACAACAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGAT
  TGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTC
  CCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAAT
  AAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATATTATGGNGTGGAGG
  GGGGTGGTATGGAGCAAGGGGCNNAGTNGGAAAGACACCTGTAGGGCCTGCGGGGTCTAT
  TGGGAACCAGCTGGAGTGCAGTGGCACAATCTGGGCTCACTGNCATCTCCGCCTCCTGGN
  GTCAAAGCGATCTCCTGCCTCAGCCNTCCCGANTNGNTGGGGATTNCAGGCATGCATGAC
  CCAGCTCAGCTAATTTTTGGTTTTTGGTAGAGACGGNGTTCACATATGGCCAGCTGGTCT
  CACTCTATTCAGTGACTACCACTGGCTCAATGCTGGATTCAGCGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001021 unedited
  ACGCCGAGCGCCGCCAATTCTCAGGGACGCGNGATGTCTTTTGTCTTCTTTCTTTGTTTT
  CTTGGGTGCGCCCACCATTTATTGCAAAATAATACAGCACTACAGCAAAAAATTCAAACA
  GGTCCCCGAGGCGTTTTGAAATTCATCCCAACTGTAGGCTGAGTGACCTGAAGGTTGGAC
  AGACTGTCGAAGCGCAAAAGCTTCAGCATTTGCTTAGTGGCAGGATCTACTTGAATAATC
  TCCTGATCCAAGGGTGACACCTCAGGAACATAATTGACTGTGCTTTGTGTGTGCTACTAC
  TGCAACTTGATGGAGATACCTGTTACTGGGGCTATGTGAATGCCTCCGTGGCGATGCGCG
  CCGCACCCCGCAGCCCTGCCGCGCAGCTCTTCGCTGGGGATAATGGCGATCTTCCCCATA
  CACACCCTTGCTACAGTGGCAACCCTTCGCCCACCCCCGTCAGCCACCCTGACATGAAGA
  CCCCGGACCTCCTTTCTCCCCCATCCGCAACCAACACACCTCCGCGGCTCCCTGTCCCCC
  ATCCCGCCGCCCGCCGGTCGTCTCTGCACCCCCCCACTCCGTCCACCCACCATCCCTCCC
  CCCTCCTGCTCGTTCCCCCACGCCCCCCCCCCCTTCATCCCCCCGCTTTTCTGTTCCTGT
  ATTCCCCATGCACCTCCTTCCCCTCCTTCCCCCCCCTCTTTCCCCACCCCTTGATCCCCT
  TTATTCCACCTCCCCACTACACCCCCCCTCCCCGGTTTCAGCTTCATCCATCCTCCCTCC
  CCCTTTCCCCTTCCTCCTTCCTCACCATCCTCCCCCCCCCTCCCCCTCCCCGTTTTTCCC
  CCACCTCCGACCGCCCTTTTCCCCCCGCCCCTTCCGTTTGCCCCCTATTTTCCCCCCCTC
  CCCTATCGTCTCCCCTCCTCCCTTTTCCTTCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001021
• Insert Size: 500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001021.3, NP_001012.1
• RefSeq Size: 511 bp
• RefSeq ORF: 408 bp
• Locus ID: 6218
• UniProt ID: P08708
• Cytogenetics: 15q25.2
• Domains: Ribosomal_S17e
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S17E family of ribosomal proteins and is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia 4. Alternative splicing of this gene results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Apr 2014]
  Transcript Variant: This variant (1) encodes the supported protein.