RPS13 (NM_001017) Human Untagged Clone

CAT#: SC119524

RPS13 (untagged)-Human ribosomal protein S13 (RPS13)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPS13
• Synonyms: S13
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119524 sequence for NM_001017 edited (data generated by NextGen Sequencing)
  ATGGGTCGCATGCATGCTCCCGGGAAGGGCCTGTCCCAGTCGGCTTTACCCTATCGACGC
  AGCGTCCCCACTTGGTTGAAGTTGACATCTGACGACGTGAAGGAGCAGATTTACAAACTG
  GCCAAGAAGGGCCTTACTCCTTCACAGATCGGTGTAATCCTGAGAGATTCACATGGTGTT
  GCACAAGTACGTTTTGTGACAGGCAATAAAATTTTAAGAATTCTTAAGTCTAAGGGACTT
  GCTCCTGATCTTCCTGAAGATCTCTACCATTTAATTAAGAAAGCAGTTGCTGTTCGAAAG
  CATCTTGAGAGGAACAGAAAGGATAAGGATGCTAAATTCCGTCTGATTCTAATAGAGAGC
  CGGATTCACCGTTTGGCTCGATATTATAAGACCAAGCGAGTCCTCCCTCCCAATTGGAAA
  TATGAATCATCTACAGCCTCTGCCCTGGTCGCATAA
  
  Clone variation with respect to NM_001017.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001017 unedited
  CGGCCGCGAATTCGGCACCAGCCGCCATCATGGGTCGCATGCATGCTCCCGGGAAGGGCC
  TGTCCCAGTCGGCTTTACCCTATCGACGCAGCGTCCCCACTTGGTTGAAGTTGACATCTG
  ACGACGTGAAGGAGCAGATTTACAAACTGGCCAAGAAGGGCCTTACTCCTTCACAGATCG
  GTGTAATCCTGAGAGATTCACATGGTGTTGCACAAGTACGTTTTGTGACAGGCAATAAAA
  TTTTAAGAATTCTTAAGTCTAAGGGACTTGCTCCTGATCTTCCTGAAGATCTCTACCATT
  TAATTAAGAAAGCAGTTGCTGTTCGAAAGCATCTTGAGAGGAACAGAAAGGATAAGGATG
  CTAAATTCCGTCTGATTCTAATAGAGAGCCGGATTCACCGTTTGGCTCGATATTATAAGA
  CCAAGCGAGTCCTCCCTCCCAATTGGAAATATGAATCATCTACAGCCTCTGCCCTGGTCG
  CATAAATTTGTCTGTGTACTCAAGCAATAAAATGATTGTTTAACTAAAAAAAAAAAAAAA
  AAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGG
  CATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCC
  ACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTAT
  ATATTATGGGGTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001017 unedited
  CTTGGACCGCGGCCGCATCTANATCGAGTTTTTTTTTTTTTTTTTTTAGTTAAACAATCA
  TTTTATTGCTTGAGTACACAGACAAATTTATGCGACCAGGGCAGAGGCTGTAGATGATTC
  ATATTTCCAATTGGGAGGGAGGACTCGCTTGGTCTTATAATATCGAGCCAAACGGTGAAT
  CCGGCTCTCTATTAGAATCAGACGGAATTTAGCATCCTTATCCTTTCTGTTCCTCTCAAG
  ATGCTTTCGAACAGCAACTGCTTTCTTAATTAAATGGTAGAGATCTTCAGGAAGATCAGG
  AGCAAGTCCCTTAGACTTAAGAATTCTTAAAATTTTATTGCCTGTCACAAAACGTACTTG
  TGCAACACCATGTGAATCTCTCAGGATTACACCGATCTGTGAAGGAGTAAGGCCCTTCTT
  GGCCAGTTTGTAAATCTGCTCCTTCACGTCGTCAGATGTCAACTTCAACCAAGTGGGGAC
  GCTGCGTCGATAGGGTAAAGCCGACTGGGACAGGCCCTTCCCGGGAGCATGCATGCGACC
  CATGATGGCGGCTGGTGCCCACTTCACGGCCGCCCTATAGCGAGTCGTACCCAAAATTCT
  CACCGCCACTAAACGAGCCCCGTTCATATGCACCTTACCCCGTCACCCCCCTATCCGCCC
  TTTACACTCCCCGGGAGTCGACCCACATCCACCTTTCCCCCCGTACCACCCCNTCCCCCA
  TCGCCCCGCACGTCCACCCCCCCCCAACCTCGCACCCCCCCGCAACCACTCCCTCTCTCC
  CCCCTATCCACTCACCCCCCTTTTCCACCCCATTCTACCATTCCTCACATCCTTTTCCCC
  CCCCCCCCACCCTTTTTCCCCCCCCCCCCCCTCCCCCCTCCTTCCCCACGCCTCACCCGC
  CCTCCACCGACCTCTCGCCCCCTTTATTCCCCCCCCACCCCTCTCCTTCTTCTTATCCCA
  TATTCTCTCCGCGTCTTACCCCCACCCACCCTCCCCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001017
• Insert Size: 480 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017.2, NP_001008.1
• RefSeq Size: 529 bp
• RefSeq ORF: 456 bp
• Locus ID: 6207
• UniProt ID: P62277
• Cytogenetics: 11p15.1
• Domains: Ribosomal_S15
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S15P family of ribosomal proteins. It is located in the cytoplasm. The protein has been shown to bind to the 5.8S rRNA in rat. The gene product of the E. coli ortholog (ribosomal protein S15) functions at early steps in ribosome assembly. This gene is co-transcribed with two U14 small nucleolar RNA genes, which are located in its third and fifth introns. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]