RPLP2 (NM_001004) Human Untagged Clone

CAT#: SC119511

RPLP2 (untagged)-Human ribosomal protein, large, P2 (RPLP2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPLP2
• Synonyms: D11S2243E; LP2; P2; RPP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119511 sequence for NM_001004 edited (data generated by NextGen Sequencing)
  ATGCGCTACGTCGCCTCCTACCTGCTGGCTGCCCTAGGGGGCAACTCCTCCCCCAGCGCC
  AAGGACATCAAGAAGATCTTGGACAGCGTGGGTATCGAGGCGGACGACGACCGGCTCAAC
  AAGGTTATCAGTGAGCTGAATGGAAAAAACATTGAAGACGTCATTGCCCAGGGTATTGGC
  AAGCTTGCCAGTGTACCTGCTGGTGGGGCTGTAGCCGTCTCTGCTGCCCCAGGCTCTGCA
  GCCCCTGCTGCTGGTTCTGCCCCTGCTGCAGCAGAGGAGAAGAAAGATGAGAAGAAGGAG
  GAGTCTGAAGAGTCAGATGATGACATGGGATTTGGCCTTTTTGATTAA
  
  Clone variation with respect to NM_001004.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001004 unedited
  AAGGTGCACATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGCCACCG
  AGGTCGCACGCGTGAGACTTCTCCGCCGCCTCCGCCGCAGACGCCGCCGCGATGCGCTAC
  GTCGCCTCCTACCTGCTGGCTGCCCTAGGGGGCAACTCCTCCCCCAGCGCCAAGGACATC
  AAGAAGATCTTGGACAGCGTGGGTATCGAGGCGGACGACGACCGGCTCAACAAGGTTATC
  AGTGAGCTGAATGGAAAAAACATTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCC
  AGTGTACCTGCTGGTGGGGCTGTAGCCGTCTCTGCTGCCCCAGGCTCTGCAGCCCCTGCT
  GCTGGTTCTGCCCCTGCTGCAGCAGAGGAGAAGAAAGATGAGAAGAAGGAGGAGTCTGAA
  GAGTCAGATGATGACATGGGATTTGGCCTTTTTGATTAAATTCCTGCTCCCCTGCAAATA
  AAGCCTTTTTACACATCTAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCG
  CGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGC
  CTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAA
  GTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGTGGAGGGGGGGGGG
  GATGGGACCCAGGGCGCAACCTGTCCAAACAATCCGTAAGGTCCCGCCCGANCTTAACCG
  GTACCCCCCAGCTTTCCGAATGGCACTAAATATAAACCACACACGAACCCCCGCCCACCT
  GACCAACATCCCTAACTCCTCCACCATCAACCCAAAATATTACCAACCACACACAACCCC
  AACAAAAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001004 unedited
  AAAACCTAGCTCTGNACCGCGCGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTT
  TTAGATGGGTAAAAAGGCTTTATTTGCAGGGGAGCAGGAATTTAATCAAAAAGGCCAAAT
  CCCATGTCATCATCTGACTCTTCAGACTCCTCCTTCTTCTCATCTTTCTTCTCCTCTGCT
  GCAGCAGGGGCAGAACCAGCAGCAGGGGCTGCAGAGCCTGGGGCAGCAGAGACGGCTACA
  GCCCCACCAGCAGGTACACTGGCAAGCTTGCCAATACCCTGGGCAATGACGTCTTCAATG
  TTTTTTCCATTCAGCTCACTGATAACCTTGTTGAGCCGGTCGTCGTCCGCCTCGATACCC
  ACGCTGTCCAAGATCTTCTTGATGTCCTTGGCGCTGGGGGAGGAGTTGCCCCCTAGGGCA
  GCCAGCAGGTAGGAGGCGACGTAGCGCATCGCGGCGGCGTCTGCGGCGGAGGCGGCGGAA
  AAGTCTCACGCGTGCGACCTCGGTGGCCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGA
  GTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACC
  GTACACGCCTACCGNCCATTTGCGTCAACGGGGCGGGGTTATTACGACATTTTGGAAAGT
  CCCGTTGATTNTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAA
  ATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGGTGTACTGGCCAAACCGCATCACCA
  TGGGTATAGCGATGACTAATACGTANATGTACTGCCCAGTAAGAAAGTCCCGTAAGGTCA
  TGTACTGGGCATAATGCCAGCGGGCCATTTACCGTCATTGACGTCATAAGGGGCGGACTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001004
• Insert Size: 470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004.2, NP_000995.1
• RefSeq Size: 482 bp
• RefSeq ORF: 348 bp
• Locus ID: 6181
• UniProt ID: P05387
• Cytogenetics: 11p15.5
• Domains: 60s_ribosomal
• Protein Families: Druggable Genome
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein that is a component of the 60S subunit. The protein, which is a functional equivalent of the E. coli L7/L12 ribosomal protein, belongs to the L12P family of ribosomal proteins. It plays an important role in the elongation step of protein synthesis. Unlike most ribosomal proteins, which are basic, the encoded protein is acidic. Its C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P1. The P2 protein can interact with P0 and P1 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]