RPL29 (NM_000992) Human Untagged Clone

CAT#: SC119499

RPL29 (untagged)-Human ribosomal protein L29 (RPL29)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL29
• Synonyms: HIP; HUMRPL29; L29; RPL29P10; RPL29_3_370
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119499 sequence for NM_000992 edited (data generated by NextGen Sequencing)
  ATGGCCAAGTCCAAGAACCACACCACACACAACCAGTCCCGAAAATGGCACAGAAATGGT
  ATCAAGAAACCCCGATCACAAAGATACGAATCTCTTAAGGGGGTGGACCCCAAGTTCCTG
  AGGAACATGCGCTTTGCCAAGAAGCACAACAAAAAGGGCCTAAAGAAGATGCAGGCCAAC
  AATGCCAAGGCCATGAGTGCACGTGCCGAGGCTATCAAGGCCCTCGTAAAGCCCAAGGAG
  GTTAAGCCCAAGATCCCAAAGGGTGTCAGCCGCAAGCTCGATCGACTTGCCTACATTGCC
  CACCCCAAGCTTGGGAAGCGTGCTCGTGCCCGTATTGCCAAGGGGCTCAGGCTGTGCCGG
  CCAAAGGCCAAGGCCAAGGCCAAGGCCAAGGATCAAACCAAGGCCCAGGCTGCAGCCCCA
  GCTTCAGTTCCAGCTCAGGCTCCCAAACGTACCCAGGCCCCTACAAAGGCTTCAGAGTAG
  
  Clone variation with respect to NM_000992.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000992 unedited
  GAGGTTCGATTTTTGTNATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGCTT
  CGGGNACCGCGGCTTATGGTGCAGACTGGCCAAGTCCAAAAACCACACCACACACAACCA
  GTCCCGAAATGGCACAGAAATGGTATCAAGAAACCCCGATCACAAAGATACGAATCTCTT
  AAGGGGGTGGACCCCAAGTTCCTGAGGAACATGCGCTTTGCCAAGAAGCACAACAAAAAG
  GGCCTAAAGAAGATGCAGGCCAACAATGCCAAGGCCATGAGTGCACGTGCCGAGGCTATC
  AAGGCCCTCGTAAAGCCCAAGGAGGTTAAGCCCAAGATCCCAAAGGGTGTCAGCCGCAAG
  CTCGATCGACTTGCCTACATTGCCCACCCCAAGCTTGGGAAGCGTGCTCGTGCCCGTATT
  GCCAAGGGGCTCAGGCTGTGCCGGCCAAAGGCCAAGGCCAAGGCCAAGGCCAAGGATCAA
  ACCAAGGCCCAGGCTGCAGCCCCAGCTTCAGTTCCAGCTCAGGCTCCCAAACGTACCCAG
  GCCCCTACAAAGGCTTCAGAGTAGATATCTCTGCCAACATGAGGACAGAAGGACTGGTGC
  GACCCCCCACCCCCGCCCCTGGGCTACCATCTGCATGGGGCTGGGGTCCTCCTGTGCTAT
  TTGTACAAATAAACCTGAGGCAGGAANCNNNNANCNCCCNNNNTNACACAAAAAAAAAAA
  AACAACCTCGACTCTAGATTGCGGCCGCCGTCATAGCTGTTTCCTGAACAGATCCCGGTT
  GGCATCCTTGTGACCCCTCCCCAGGCCTTTCCTGGCCCTGAAATTTGCCCTCCCATGCCC
  ACACCCTGTCCCTATAAAAATAGTTGCTTATTTTGTCTGACTAGGGCCTTCTATAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000992 unedited
  TTTATGCNACCGCGGCCGCAATCTTAGNANCGAGCTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTCCCTGCCCAAGGGTTAATTGGGCCAAAAAGCACAGGAGGACCC
  CACCCCCATGCAAAGGGAACCCCAGGGGCGGGGGGGGGGGGCCCCACCAGCCCTTCTGCC
  CTAATGTGGGCAAAAATATCTACTTTGAACCCTTTGAAGGGGCCTGGTTACGTTGGGAAC
  CCTGACCTGAAACGGAAGCTGGGCCTGCATCTTGGCCCTTGGTTGGACCCTTGGCCTTGG
  CCTTGGCCTTGGCCTTTGGCCGGCACAGCCTGACCCCCTTGGAAATACGGGCACAAGCAC
  CCTTCCAAAGCTGGGGGGGGGCAATGTAGGCAAGCCAATCGAGCTTGCGGTTGACACCTT
  TTGGAATCTTGGGCTAAACCTCTTTGGGCTTAACGAGGCCCTTGATAGCCCCGGCACGTG
  CACTAATGGCCTTGGAATTGTTGGCCTGCATCTTCTTTAGGCCCTTTTTGTTGGGCTTCT
  GGGCAAAGCCCATGTCCCTAAAGAACTTGGGGCCCCCCCCCTTAAAAGATTTGAATCTTT
  GGGACGGGGGTTTTTGAAACCATTTTTTGCCCATTTTCGGAACCGGTTGCGGGGGGGGGG
  TTTTTGGACTTGCCCAGGCTGCCCATAACCCCGGGCTCCCAAAGGCCCTTGTGCCAAATT
  CCGGGCCCGCCTAATAGGACCCGATTCACAAAATTCCGCGGGCCAACAAACCGAGTTCCC
  GTCCAAAAAACCCCCAACGGACCGCCTCAACCGCCATTGGCCCCCAGGGCCCGGGCGTTA
  AACCCACCTCTTGCAACGCTCCCCTCCTCCTCTGTGCCAACAAAAAATTCCTCTCCCCCT
  CCAAGGGCGCGCTCTCTTTCCCCACCCCTCCTCTCCTTCCCCCTCCCCACCCTTCCTCTC
  T
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000992
• Insert Size: 690 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000992.2, NP_000983.1
• RefSeq Size: 737 bp
• RefSeq ORF: 480 bp
• Locus ID: 6159
• UniProt ID: P47914
• Cytogenetics: 3p21.2
• Domains: Ribosomal_L29e
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29E family of ribosomal proteins. The protein is also a peripheral membrane protein expressed on the cell surface that directly binds heparin. Although this gene was previously reported to map to 3q29-qter, it is believed that it is located at 3p21.3-p21.2. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]