RPL30 (NM_000989) Human Untagged Clone

CAT#: SC119495

RPL30 (untagged)-Human ribosomal protein L30 (RPL30)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL30
• Synonyms: L30
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000989, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTGGCCGCAAAGAAGACGAAAAAGTCGCTGGAGTCGATCAACTCTAGGCTCCAACTCGTTATGAAAA
  GTGGGAAGTACGTCCTGGGGTACAAGCAGACTCTGAAGATGATCAGACAAGGCAAAGCGAAATTGGTCAT
  TCTCGCTAACAACTGCCCAGCTTTGAGGAAATCTGAAATAGAGTACTATGCTATGTTGGCTAAAACTGGT
  GTCCATCACTACAGTGGCAATAATATTGAACTGGGCACAGCATGCGGAAAATACTACAGAGTGTGCACAC
  TGGCTATCATTGATCCAGGTGACTCTGACATCATTAGAAGCATGCCAGAACAGACTGGTGAAAAGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000989 unedited
  GCACGAGGCTGCTGTTGGTTGGGGGCCGTCCCGCTCCTAAGCAGGAAGAGGTGGCCGCAA
  AGAAACGAAAAATCGCTGGAGTCGATCAACTCTAGGCTCCAACTCGTTATGAAAAGTGGG
  AAGTACGTCCTGGGGTACAAGCAGACTCTGAAGATGATCAGACAAGGCAAAGCGAAATTG
  GTCATTCTCGCTAACAACTGCCCAGCTTTGAGGAAATCTGAAATAGAGTACTATGCTATG
  TTGGCTAAAACTGGTGTCCATCACTACAGTGGCAATAATATTGAACTGGGCACAGCATGC
  GGAAAATACTACAGAGTGTGCACACTGGCTATCATTGATCCAGGTGACTCTGACATCATT
  AGAAGCATGCCAGAACAGACTGGTGAAAAGTAAACCTTTTCACCTACAAAATTTCACCTG
  CAAACCTTAAACCTGCAAAATTTTCCTTTAATAAAATTTGCTTGTTTTAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000989 unedited
  NGATCGATGTNTNTAATACGGCCTAATGGGCAGGTTTAAGGCCTGCAGGTGAAATTTTGT
  AGGTGAAAAGGTTTACTTTTCACCAGTCTGTTCTGGCATGCTTCTAATGATGTCAGAGTC
  ACCTGGATCAATGATATCCAGTGTGCACACTCTGTAGTATTTTCCGCATGCTGTGCCCAG
  TTCAATATTATTGCCACTGTAGTGATGGACACCAGTTTTATTCAACATAGCATATGTACT
  CTATTTCAGATTTCCTCAAAGCTGGGCAGTTGTTAGCGAGAATGACCAATTTCGCTTTGC
  CTTGTCTGATCATCTTCAGAGTCTGCTTGTACCCCAGGACGTACTTCCCACTTTTCATAA
  CGAGCTGGAGCCTAGAGTTGATCGACTCCAGCGACTTTTTCGTCTTCTTTGCGGCCACCA
  TTTTCCTGCCTTAGGAGCGGGACGGCCCCCAACCAACAGCATTCTCGTGCCGAATTTCCG
  GCCGCTCTATAGTGAGTTCTTTTTCACAATTCTGACGGTTTTCTAAACGAGCTCTTGCTT
  ATTATACCTCCCACCGTCACGCCTACCGCCCTTTGCGTCTCGGGGCGGGGTTTTTTCGAC
  TTTTGGGTAAGTCCCGTTGGTTTTTGTGCCCCAACAACCCCCCTTGCCCGCCATGGGGTG
  GTTACTTGGCATTTCCCGGGTGTCTACCGCTTTCCCCGCCCCTTGGTGCCCTGCCTAATC
  CGCCTTCCCTGTGTCCTTGCCGTGGCCCACCCCTTTCAGTTCTGCCCAGCTTGTAAATCT
  CCGCTCGGTCATGTTCTCGGGCTTATTGCCCGCGGGCCCTTTCCCGTCCTTGCCGTATTA
  GGGATGGCACTTGCCTTTGTTCCTCTGTATCTCGCCCAGTGCGCTTCTACCCAAAAAATC
  AACACAAAAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000989
• Insert Size: 570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000989.2, NP_000980.1
• RefSeq Size: 524 bp
• RefSeq ORF: 348 bp
• Locus ID: 6156
• UniProt ID: P62888
• Cytogenetics: 8q22.2
• Domains: Ribosomal_L7Ae
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]