RPL27 (NM_000988) Human Untagged Clone

CAT#: SC119494

RPL27 (untagged)-Human ribosomal protein L27 (RPL27)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL27
• Synonyms: DBA16; L27
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119494 sequence for NM_000988 edited (data generated by NextGen Sequencing)
  ATGGGCAAGTTCATGAAACCTGGGAAGGTGGTGCTTGTCCTGGCTGGACGCTACTCCGGA
  CGCAAAGCTGTCATCGTGAAGAACATTGATGATGGCACCTCAGATCGCCCCTACAGCCAT
  GCTCTGGTGGCTGGAATTGACCGCTACCCCCGCAAAGTGACAGCTGCCATGGGCAAGAAG
  AAGATCGCCAAGAGATCAAAGATAAAATCTTTTGTGAAAGTGTATAACTACAATCACCTA
  ATGCCCACAAGGTACTCTGTGGATATCCCCTTGGACAAAACTGTCGTCAATAAGGATGTC
  TTCAGAGATCCTGCTCTTAAACGCAAGGCCCGACGGGAGGCCAAGGTCAAGTTTGAAGAG
  AGATACAAGACAGGCAAGAACAAGTGGTTCTTCCAGAAACTGCGGTTTTAG
  
  Clone variation with respect to NM_000988.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000988 unedited
  GGTGCAAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGTGG
  TTGCTGCCGAAATGGGCAAGTTCATGAAACCTGGGAAGGTGGTGCTTGTCCTGGCTGGAC
  GCTACTCCGGACGCAAAGCTGTCATCGTGAAGAACATTGATGATGGCACCTCAGATCGCC
  CCTACAGCCATGCTCTGGTGGCTGGAATTGACCGCTACCCCCGCAAAGTGACAGCTGCCA
  TGGGCAAGAAGAAGATCGCCAAGAGATCAAAGATAAAATCTTTTGTGAAAGTGTATAACT
  ACAATCACCTAATGCCCACAAGGTACTCTGTGGATATCCCCTTGGACAAAACTGTCGTCA
  ATAAGGATGTCTTCAGAGATCCTGCTCTTAAACGCAAGGCCCGACGGGAGGCCAAGGTCA
  AGTTTGAAGAGAGATACAAGACAGGCAAGAACAAGTGGTTCTTCCAGAAACTGCGGTTTT
  AGATGCTTTGTTTTGATCATTAAAAATTATAAAGAAAAAAAAAAAAAAAAAACTCGACTC
  TAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGAC
  CCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTC
  CTAATAAAATTAAGTTGCATCATTNTGTCTGACTAGGTGTCCTTCTATAATATTATGGGG
  TGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTTAGGCCTGCGGG
  GTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATTCTGGCTCACTGCAATCTCCCCC
  TCCTGGGTCCAAGCGATNCTTCTGCCTCAACCTNCCCGANTTGTTGGGATTCCAGGCTGG
  CTGACCAGGCCCAAGTAATTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000988 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTTTATAATTTTTAATGATCAAA
  ACAAAGCATCTAAAACCGCAGTTTCTGGAAGAACCACTTGTTCTTGCCTGTCTTGTATCT
  CTCTTCAAACTTGACCTTGGCCTCCCGTCGGGCCTTGCGTTTAAGAGCAGGATCTCTGAA
  GACATCCTTATTGACGACAGTTTTGTCCAAGGGGATATCCACAGAGTACCTTGTGGGCAT
  TAGGTGATTGTAGTTATACACTTTCACAAAAGATTTTATCTTTGATCTCTTGGCGATCTT
  CTTCTTGCCCATGGCAGCTGTCACTTTGCGGGGGTAGCGGTCAATTCCAGCCACCAGAGC
  ATGGCTGTAGGGGCGATCTGAGGTGCCATCATCAATGTTCTTCACGATGACAGCTTTGCG
  TCCGGAGTAGCGTCCAGCCAGGACAAGCACCACCTTCCCAGGTTTCATGAACTTGCCCAT
  TTCGGCAGCAACCACCCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACA
  CAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAAACCTCCCACCGTACACGCCTA
  CCGTCCATTTGCGTAACGGGGCCGGGTTATTACCACCTTTTGGAAAGTCCCGTTGTTTTG
  GTGCCAAACCAAACCTCCATTGGAGGTAATGGGGTGGAGACTCGGGAATCCCCGGAGTCA
  ACCGTTCCCCAGCCCCTGGGTGACTGGCACACCGGCCCCCATGGTATAGCATCACTTATA
  CGAAACGCCCCCCATTCGGCCAGCCCCCCAGGCGCTGATGGGTTTTATGTTTTTGGCGAT
  TCTACGGACACGCCGCCCAAGGGGCGCCGCGCGTACCACCCCTCATCCCCTCCACTCTTA
  TTCCCCGCACTTTCCCCCTACCGTCGTGCAGCCCTCTTGGTTGTTCGCGCCTTCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000988
• Insert Size: 510 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000988.2, NP_000979.1
• RefSeq Size: 513 bp
• RefSeq ORF: 411 bp
• Locus ID: 6155
• UniProt ID: P61353
• Cytogenetics: 17q21.31
• Domains: Ribosomal_L27e, KOW, KOW
• Protein Families: Druggable Genome
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L27e family of ribosomal proteins and a component of the 60S subunit. A splice site mutation in this gene has been identified in a Diamond-Blackfan anemia (DBA) patient. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Mar 2017]
  Transcript Variant: This variant (1) encodes the protein. Variants 1-3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.