RPL17 (NM_000985) Human Untagged Clone

CAT#: SC119491

RPL17 (untagged)-Human ribosomal protein L17 (RPL17), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL17
• Synonyms: L17; PD-1; RPL23
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119491 sequence for NM_000985 edited (data generated by NextGen Sequencing)
  ATGGTTCGCTATTCACTTGACCCGGAGAACCCCACGAAATCATGCAAATCAAGAGGTTCC
  AATCTTCGTGTTCACTTTAAGAACACTCGTGAAACTGCTCAGGCCATCAAGGGTATGCAT
  ATACGAAAAGCCACGAAGTATCTGAAAGATGTCACTTTACAGAAACAGTGTGTACCATTC
  CGACGTTACAATGGTGGAGTTGGCAGGTGTGCGCAGGCCAAGCAATGGGGCTGGACACAA
  GGTCGGTGGCCCAAAAAGAGTGCTGAATTTTTGCTGCACATGCTTAAAAACGCAGAGAGT
  AATGCTGAACTTAAGGGTTTAGATGTAGATTCTCTGGTCATTGAGCATATCCAAGTGAAC
  AAAGCACCTAAGATGCGCCGCCGGACCTACAGAGCTCATGGTCGGATTAACCCATACATG
  AGCTCTCCCTGCCACATTGAGATGATCCTTACGGAAAAGGAACAGATTGTTCCTAAACCA
  GAAGAGGAGGTTGCCCAGAAGAAAAAGATATCCCAGAAGAAACTGAAGAAACAAAAACTT
  ATGGCACGGGAGTAA
  
  Clone variation with respect to NM_000985.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000985 unedited
  CGGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCGCGAGGGCAGCCTG
  AGGTGATCTGTGAAAATGGTTCGCTATTCACTTGACCCGGAGAACCCCACGAAATCATGC
  AAATCAAGAGGTTCCAATCTTCGTGTTCACTTTAAGAACACTCGTGAAACTGCTCAGGCC
  ATCAAGGGTATGCATATACGAAAAGCCACGAAGTATCTGAAAGATGTCACTTTACAGAAA
  CAGTGTGTACCATTCCGACGTTACAATGGTGGAGTTGGCAGGTGTGCGCAGGCCAAGCAA
  TGGGGCTGGACACAAGGTCGGTGGCCCAAAAAGAGTGCTGAATTTTTGCTGCACATGCTT
  AAAAACGCAGAGAGTAATGCTGAACTTAAGGGTTTAGATGTAGATTCTCTGGTCATTGAG
  CATATCCAAGTGAACAAAGCACCTAAGATGCGCCGCCGGACCTACAGAGCTCATGGTCGG
  ATTAACCCATACATGAGCTCTCCCTGCCACATTGAGATGATCCTTACGGAAAAGGAACAG
  ATTGTTCCTAAACCAGAAGAGGAGGTTGCCCAGAAGAAAAAGATATCCCAGAAGAAACTG
  AAGAAACAAAAACTTATGGCACGGGAGTAAATTCAGCATTAAAATAAATGTAATTAAAAG
  GAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGA
  ACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTG
  CCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACT
  AGGTGTCCCTCTATAATATATGGGTGGAGGGGGGTGGGGGATTGGACAAAGGGGCAACTT
  GGCAAAACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000985 unedited
  AATTTTTGCCCGCGGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTCCTTTTAATT
  ACATTTTTTTTAATGCTGAATTTACTGCCCGGGCCATAAGTTTTTGTTTCTTCAGTTTCT
  TCTGGGATATCTTTTTCTTCTGGGCAACCTCCTCTTCTGGTTTAGGAACAATCTGTTCCT
  TTTCCGTAAGGATCATCTCAATGTGGCAGGGAGAGCTCATGTTTGGGTTAATCCGACCAT
  GAGCTCTGTGGGTCCGGCGGCGCATTTTAGGTGCTTTGTTCACTTGGATATGCTCAATGA
  CCAGAGAATCTACATCTAAACCCTTAAGTTCAGCATTACTCTCTGCGTTTTTAAGCATGT
  GCAGCAAAAATTCAGCACTCTTTTTGGGCCACCGACCTTGTGTCCAGCCCCATTGCTTGG
  CCTGCGCACACCTGCCAACTCCACCATTGTAACGTCGGAATGGTACACACTGTTTCTGTA
  AAGTGACATCTTTCAGATACTTCGTGGCTTTTCGTATATGCATACCCTTGATGGCCTGAG
  CAGTTTCACGAGTGTTCTTAAAGTGAACACGAAGATTGGAACCTCTTGATTTGCATGATT
  TCGTGGGGTTCTCCGGGTCAAGTGAATAGCGAACCATTTTCACAGATCACCTCAGGCTGC
  CCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAATTCTGACGGTTCAC
  TAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTAAC
  GGGGGCGGGGTTATTACCACATTTTGGAAGTCCCGGTGATTTTGGTGCCCAAACAAACTC
  CCATTGACGCCATGGGGTGGAAACCTTGGAATCCCCGGGATCAAACCGCTATCCCCCCCC
  ATGGTGTACTGCCAAACCGCTTAACCTGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000985
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000985.2, NP_000976.1
• RefSeq Size: 898 bp
• RefSeq ORF: 555 bp
• Locus ID: 6139
• UniProt ID: P18621
• Cytogenetics: 18q21.1
• Domains: Ribosomal_L22
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream C18orf32 (chromosome 18 open reading frame 32) gene. [provided by RefSeq, Dec 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1-7 all encode isoform a.